Literature for peptidase S01.001: chymotrypsin A (cattle-type)

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Pharma

(Topics flags: S Structure, A Assay, P Specificity, I Inhibitor, V Review. To select only the references relevant to a single topic, click the link above. See explanation.)

2026

Zhou,S., Liu,C., Shi,H. and Gao,L. Highly Sensitive Detection of Chymotrypsin Using Gold Nanoclusters with Peptide Sensors Micromachines (Basel) (2026) 17 PubMed Europe PubMed DOI PMC EPMC

2025

Stefan,A. and Hochkoeppler,A. The catalytic action of enzymes exposed to charged substrates outperforms the activity exerted on their neutral counterparts Biochem Biophys Res Commun (2025) 751, 151436-151436. PubMed Europe PubMed DOI

2024

Ansari,N.K., Khan,H.S. and Naeem,A. Doxorubicin as a Drug Repurposing for Disruption of alpha-Chymotrypsinogen-A Aggregates Protein J (2024) 43, 842-857. PubMed Europe PubMed DOI
Gajardo-Parra,N.F., Cea-Klapp,E., Chandra,A., Canales,R., Garrido,J.M., Held,C. and Guajardo,N.V. Assessing the effect of deep eutectic solvents on alpha-chymotrypsin thermal stability and activity ChemSusChem (2024) , e202401414-e202401414. PubMed Europe PubMed DOI
Gholizadeh,M., Shareghi,B. and Farhadian,S. Revealing the interaction between alpha-chymotrypsin and eugenol: An integrated multi-spectral and dynamic simulation approach Int J Biol Macromol (2024) 277, 134504-134504. PubMed Europe PubMed DOI
Patel,C.K. and Mukherjee,T.K. Biomolecular Condensation of Trypsin Prevents Autolysis and Promotes Ca(2+)-Mediated Activation of Esterase Activity Biomacromolecules (2024) 25, 6082-6092. PubMed Europe PubMed DOI
Shirazi,S.H., Rizvi,F., Sherwani,Z.A., Siddiqui,A.R., Ul-Haq,Z., Siddiqui,H., Choudhary,M.I. and Ahmad,M.S. Synthesis of isoniazid derivatives and evaluation of their alpha-chymotrypsin inhibitory effect through in silico guided in vitro studies Biochem Biophys Res Commun (2024) 735, 150805-150805. PubMed Europe PubMed DOI I
Sindhikara,D., Iskandar,S.E., Guan,L., Maini,R., Hipolito,C.J., Sun,C., Vasicek,L.A., Weinglass,A. and Saldanha,S.A. Analysis and Prediction of Chymotrypsin Substrate Preferences through Large Data Acquisition with Target-Free mRNA Display Chembiochem (2024) , e202400760-e202400760. PubMed Europe PubMed DOI
Yadav,B., Yadav,N. and Venkatesu,P. Unravelling the stabilization mechanism of mono-, di- and tri-cholinium citrate-ethylene glycol DESs towards alpha-chymotrypsin for preservation and activation of the enzyme Phys Chem Chem Phys (2024) 26, 28025-28036. PubMed Europe PubMed DOI
Ying,Y. and Li,H. Native top-down mass spectrometry for monitoring the rapid chymotrypsin catalyzed hydrolysis reaction Anal Chim Acta (2024) 1285, 341971-341971. PubMed Europe PubMed DOI

2023

Solovyev,M., Kashinskaya,E. and Gisbert,E. A meta-analysis for assessing the contributions of trypsin and chymotrypsin as the two major endoproteases in protein hydrolysis in fish intestine Comp Biochem Physiol A Mol Integr Physiol (2023) 278, 111372-111372. PubMed Europe PubMed DOI
Vreeke,G.J.C., Vincken,J.P. and Wierenga,P.A. The path of proteolysis by bovine chymotrypsin Food Res Int (2023) 165, 112485-112485. PubMed Europe PubMed DOI

2022

Balabushevich,N.G., Kovalenko,E.A., Filatova,L.Y., Kirzhanova,E.A., Mikhalchik,E.V., Volodkin,D. and Vikulina,A.S. Hybrid Mucin-Vaterite Microspheres for Delivery of Proteolytic Enzyme Chymotrypsin Macromol Biosci (2022) 22, e2200005-e2200005. PubMed Europe PubMed DOI
Biswas,P., Adhikari,A., Pal,U., Mondal,S., Mukherjee,D., Ghosh,R., Obaid,R.J., Moussa,Z., Choudhury,S.S., Ahmed,S.A., Das,R. and Pal,S.K. A combined spectroscopic and molecular modeling Study on structure-function-dynamics under chemical modification: Alpha-chymotrypsin with formalin preservative Front Chem (2022) 10, 978668-978668. PubMed Europe PubMed DOI PMC EPMC
Gul,S. and Khan,A.M. Molecular docking studies of isolated marine natural products against alpha-chymotrypsin Nat Prod Res (2022) , 1-4. PubMed Europe PubMed DOI
Lan,T., Tian,Q.Q., Li,M.H. and He,W. Activatable endoplasmic reticulum-targeted NIR fluorescent probe with a large Stokes shift for detecting and imaging chymotrypsin Analyst (2022) 147, 4098-4104. PubMed Europe PubMed DOI
Liu,K. Method development and optimization for measuring chymotrypsin and chymotrypsin inhibitor activities J Food Sci (2022) 87, 2018-2033. PubMed Europe PubMed DOI
Liu,S.Y., Zou,X., Gao,X. and Zhang,Y.Y. De Novo Design of a Highly Selective Nonpeptide Fluorogenic Probe for Chymotrypsin Activity Sensing in a Living System Anal Chem (2022) 94, 17922-17929. PubMed Europe PubMed DOI
Nemeth,B.Z., Demcsak,A., Micsonai,A., Kiss,B., Schlosser,G., Geisz,A., Hegyi,E., Sahin-Toth,M. and Pal,G. Arg236 in human chymotrypsin B2 (CTRB2) is a key determinant of high enzyme activity, trypsinogen degradation capacity, and protection against pancreatitis Biochim Biophys Acta Proteins Proteom (2022) 1870, 140831-140831. PubMed Europe PubMed DOI PMC EPMC
Riley,M.B., Strandquist,E., Weitzel,C.S. and Driskell,J.D. Structure and activity of native and thiolated alpha-chymotrypsin adsorbed onto gold nanoparticles Colloids Surf B Biointerfaces (2022) 220, 112867-112867. PubMed Europe PubMed DOI
Song,Y., Gong,K., Yan,H., Hong,W., Wang,L., Wu,Y., Li,W., Li,W. and Cao,Z. Correction: Sj7170, a unique dual-function peptide with a specific alpha-chymotrypsin inhibitory activity and a potent tumor-activating effect from scorpion venom J Biol Chem (2022) 298, 102543-102543. PubMed Europe PubMed DOI PMC EPMC

2021

Fu,Z., Akula,S., Thorpe,M. and Hellman,L. Marked difference in efficiency of the digestive enzymes pepsin, trypsin, chymotrypsin, and pancreatic elastase to cleave tightly folded proteins Biol Chem (2021) 402, 861-867. PubMed Europe PubMed DOI
Gault,S. and Cockell,C.S. Perchlorate Salts Exert a Dominant, Deleterious Effect on the Structure, Stability, and Activity of alpha-Chymotrypsin Astrobiology (2021) 21, 405-412. PubMed Europe PubMed DOI
Gault,S., Jaworek,M.W., Winter,R. and Cockell,C.S. Perchlorate salts confer psychrophilic characteristics in alpha-chymotrypsin Sci Rep (2021) 11, 16523-16523. PubMed Europe PubMed DOI PMC EPMC
Lather,J. and George,J. Improving Enzyme Catalytic Efficiency by Co-operative Vibrational Strong Coupling of Water J Phys Chem Lett (2021) 12, 379-384. PubMed Europe PubMed DOI
Li,H., Wang,Y., He,X., Chen,J., Xu,F., Liu,Z. and Zhou,Y. A green deep eutectic solvent modified magnetic titanium dioxide nanoparticles for the solid-phase extraction of chymotrypsin Talanta (2021) 230, 122341-122341. PubMed Europe PubMed DOI
Mahmoud,G.S., Hosny,G. and Sayed,S.A. Hepatoprotective effect of trypsin/chymotrypsin against olanzapine-induced non-alcoholic steatohepatitis in rats Can J Physiol Pharmacol (2021) , 1-9. PubMed Europe PubMed DOI
Piovarci,I., Hianik,T. and Ivanov,I.N. Detection of Chymotrypsin by Optical and Acoustic Methods Biosensors (Basel) (2021) 11 PubMed Europe PubMed DOI PMC EPMC
Zou,X., Zhao,Y., Lai,C., Liang,Y. and Lin,W. A non-peptide probe for detecting chymotrypsin activity based on protection-deprotection strategy in living systems J Mater Chem B (2021) 9, 8417-8423. PubMed Europe PubMed DOI

2020

Dau,T., Bartolomucci,G. and Rappsilber,J. Proteomics Using Protease Alternatives to Trypsin Benefits from Sequential Digestion with Trypsin Anal Chem (2020) 92, 9523-9527. PubMed Europe PubMed DOI PMC EPMC
De Marco Verissimo,C., Jewhurst,H.L., Tikhonova,I.G., Urbanus,R.T., Maule,A.G., Dalton,J.P. and Cwiklinski,K. Fasciola hepatica serine protease inhibitor family (serpins): Purposely crafted for regulating host proteases PLoS Negl Trop Dis (2020) 14, e0008510-e0008510. PubMed Europe PubMed DOI
Di Giosia,M., Marforio,T.D., Cantelli,A., Valle,F., Zerbetto,F., Su,Q., Wang,H. and Calvaresi,M. Inhibition of alpha-chymotrypsin by pristine single-wall carbon nanotubes: clogging up the active site J Colloid Interface Sci (2020) 571, 174-184. PubMed Europe PubMed DOI I
Gault,S., Jaworek,M.W., Winter,R. and Cockell,C.S. High pressures increase alpha-chymotrypsin enzyme activity under perchlorate stress Commun Biol (2020) 3, 550-550. PubMed Europe PubMed DOI PMC EPMC
Hasan,S., Isar,M. and Naeem,A. Macromolecular crowding stabilises native structure of alpha-chymotrypsinogen-A against hexafluoropropanol-induced aggregates Int J Biol Macromol (2020) PubMed Europe PubMed DOI
Li,G., Wei,H., Bi,J., Ding,X., Li,L., Xu,S., Yang,G. and Ren,W. Insights into dietary switch in cetaceans: evidence from molecular evolution of proteinases and lipases J Mol Evol (2020) PubMed Europe PubMed DOI
Mosztbacher,D., Demcsak,A. and Sahin-Toth,M. Measuring digestive protease activation in the mouse pancreas Pancreatology (2020) 20, 288-292. PubMed Europe PubMed DOI A
Ostermeier,L., Oliva,R. and Winter,R. The multifaceted effects of DMSO and high hydrostatic pressure on the kinetic constants of hydrolysis reactions catalyzed by alpha-chymotrypsin Phys Chem Chem Phys (2020) 22, 16325-16333. PubMed Europe PubMed DOI
Yang,F., Totsingan,F., Dolan,E., Khare,S.D. and Gross,R.A. Protease-catalyzed l-aspartate oligomerization: substrate selectivity and computational modeling ACS Omega (2020) 5, 4403-4414. PubMed Europe PubMed DOI PMC EPMC

2019

Ramesh,B., Abnouf,S., Mali,S., Moree,W.J., Patil,U., Bark,S.J. and Varadarajan,N. Engineered chymotrypsiN for mass spectrometry-based detection of protein glycosylation ACS Chem Biol (2019) 14, 2616-2628. PubMed Europe PubMed DOI P
Schumann,N.C., Bruning,J., Marshall,A.C. and Abell,A.D. The role of N-terminal heterocycles in hydrogen bonding to alpha-chymotrypsin Bioorg Med Chem Lett (2019) 29, 396-399. PubMed Europe PubMed DOI I
Shi,Y.J., Huang,C.H., Lee,Y.C., Wang,L.J., Chiou,J.T. and Chang,L.S. Naja atra cardiotoxins enhance the protease activity of chymotrypsin Int J Biol Macromol (2019) 136, 512-520. PubMed Europe PubMed DOI

2018

Dusekova,E., Garajova,K., Yavaser,R., Varhac,R. and Sedlak,E. Hofmeister effect on catalytic properties of chymotrypsin is substrate-dependent Biophys Chem (2018) 243, 8-16. PubMed Europe PubMed DOI
Efremova,M.V., Veselov,M.M., Barulin,A.V., Gribanovsky,S.L., Le-Deygen,I.M., Uporov,I.V., Kudryashova,E.V., Sokolsky-Papkov,M., Majouga,A.G., Golovin,Y.I., Kabanov,A.V. and Klyachko,N.L. In situ observation of chymotrypsin catalytic activity change actuated by nonheating low-frequency magnetic field ACS Nano (2018) 12, 3190-3199. PubMed Europe PubMed DOI
Farhadian,S., Shareghi,B., Momeni,L., Abou-Zied,O.K., Sirotkin,V.A., Tachiya,M. and Saboury,A.A. Insights into the molecular interaction between sucrose and alpha-chymotrypsin Int J Biol Macromol (2018) 114, 950-960. PubMed Europe PubMed DOI
Luo,X., Zhu,W., Ding,L., Ye,X., Gao,H., Tai,X., Wu,Z., Qian,Y., Ruan,X., Li,J., Li,S. and Chen,Z. Bldesin, the first functionally characterized pathogenic fungus defensin with Kv1.3 channel and chymotrypsin inhibitory activities J Biochem Mol Toxicol (2018) , e22244-e22244. PubMed Europe PubMed DOI I
Wang,J., Zheng,X., Wang,W., Guo,H., Liu,R. and Zong,W. A study on the interaction between cadmium and alpha-chymotrypsin and the underlying mechanisms J Biochem Mol Toxicol (2018) , e22248-e22248. PubMed Europe PubMed DOI
Yang,L., Li,H., Wu,P., Mahal,A., Xue,J., Xu,L. and Wei,X. Dinghupeptins A-D, chymotrypsin inhibitory cyclodepsipeptides produced by a soil-derived Streptomyces J Nat Prod (2018) 81, 1928-1936. PubMed Europe PubMed DOI I

2017

Andersson,C.D., Martinez,N., Zeller,D., Rondahl,S.H., Koza,M.M., Frick,B., Ekstrom,F., Peters,J. and Linusson,A. Changes in dynamics of alpha-chymotrypsin due to covalent inhibitors investigated by elastic incoherent neutron scattering Phys Chem Chem Phys (2017) 19, 25369-25379. PubMed Europe PubMed DOI I
Ghosh,C., Mondal,T. and Bhattacharyya,K. Enzyme activity of alpha-chymotrypsin: deactivation by gold nano-cluster and reactivation by glutathione J Colloid Interface Sci (2017) 494, 74-81. PubMed Europe PubMed DOI I
Li,S., Fu,Y., Ma,X. and Zhang,Y. Label-free fluorometric detection of chymotrypsin activity using graphene oxide/nucleic-acid-stabilized silver nanoclusters hybrid materials Biosens Bioelectron (2017) 88, 210-216. PubMed Europe PubMed DOI A
Marasini,B.P., Rahim,F., Perveen,S., Karim,A., Mohammed Khan,K., Atta-Ur-Rahman and Choudhary,M.I. Synthesis, structure-activity relationships studies of benzoxazinone derivatives as alpha-chymotrypsin inhibitors Bioorg Chem (2017) 70, 210-221. PubMed Europe PubMed DOI I
Patra,A., Samanta,N., Das,D.K. and Mitra,R.K. Enhanced catalytic activity of alpha-chymotrypsin in cationic surfactant solutions: the component specificity revisited J Phys Chem B (2017) 121, 1457-1465. PubMed Europe PubMed DOI
Sirotkin,V.A. and Kuchierskaya,A.A. Preferential solvation/hydration of alpha-chymotrypsin in water-acetonitrile mixtures J Phys Chem B (2017) 121, 4422-4430. PubMed Europe PubMed DOI
Stewart,J.J. An investigation into the applicability of the semiempirical method PM7 for modeling the catalytic mechanism in the enzyme chymotrypsin J Mol Model (2017) 23, 154-154. PubMed Europe PubMed DOI
Stratton,A., Ericksen,M., Harris,T.V., Symmonds,N. and Silverstein,T.P. Mercury(II) binds to both of chymotrypsin's histidines, causing inhibition followed by irreversible denaturation/aggregation Protein Sci (2017) 26, 292-305. PubMed Europe PubMed DOI I
Wu,L., Yang,S.H., Xiong,H., Yang,J.Q., Guo,J., Yang,W.C. and Yang,G.F. Nonpeptide-based small-molecule probe for fluorogenic and chromogenic detection of chymotrypsin Anal Chem (2017) 89, 3687-3693. PubMed Europe PubMed DOI A
Zhang,S.-G., Xie,Z.-B., Liu,L.-S., Liang,M. and Le,Z.-G. Synthesis of 2,3-dihydroquinazolin-4(1H)-ones catalyzed by alpha-chymotrypsin Chin Chem Lett (2017) 28, 101-104. DOI

2016

Bagchi,D., Ghosh,A., Singh,P., Dutta,S., Polley,N., Althagafi,I.I., Jassas,R.S., Ahmed,S.A. and Pal,S.K. Allosteric inhibitory molecular recognition of a photochromic dye by a digestive enzyme: dihydroindolizine makes alpha-chymotrypsin photo-responsive Sci Rep (2016) 6, 34399-34399. PubMed Europe PubMed DOI
Garringer,M. and Qasim,M.A. Effect of osmolytes and guanidinium chloride on the enzymatic properties of bovine alpha-chymotrypsin Protein Pept Lett (2016) 23, 504-507. PubMed Europe PubMed
Giansanti,P., Tsiatsiani,L., Low,T.Y. and Heck,A.J. Six alternative proteases for mass spectrometry-based proteomics beyond trypsin Nat Protoc (2016) 11, 993-1006. PubMed Europe PubMed DOI
Kanamori,Y., Iwasaki,A., Sumimoto,S. and Suenaga,K. Urumamide, a novel chymotrypsin inhibitor with a beta-amino acid from a marine cyanobacterium Okeania sp Tetrahedron Lett (2016) 57, 4213-4216. DOI I
Liang,M., Xie,Z., Ai,F. and Le,Z. Synthesis of quinoline derivatives catalyzed by alpha-chymotrypsin Chin J Org Chem (2016) 36, 2704-2708. DOI
Ono,S., Nakai,T., Kuroda,H., Miyatake,R., Horino,Y., Abe,H., Umezaki,M. and Oyama,H. Site-selective chemical modification of chymotrypsin using peptidyl derivatives bearing optically active diphenyl 1-amino-2-phenylethylphosphonate: stereochemical effect of the diphenyl phosphonate moiety Biopolymers (2016) 106, 521-530. PubMed Europe PubMed DOI I
Schuabb,V., Winter,R. and Czeslik,C. Improved activity of alpha-chymotrypsin on silica particles - a high-pressure stopped-flow study Biophys Chem (2016) 218, 1-6. PubMed Europe PubMed DOI
Siddiqui,H., Farooq,R., Marasini,B.P., Malik,R., Syed,N., Moin,S.T., Atta-Ur-Rahman and Choudhary,M.I. Synthesis and in vitro alpha-chymotrypsin inhibitory activity of 6-chlorobenzimidazole derivatives Bioorg Med Chem (2016) 24, 3387-3395. PubMed Europe PubMed DOI I
Uritsky,N., Shokhen,M. and Albeck,A. Stepwise versus concerted mechanisms in general-base catalysis by serine proteases Angew Chem Int Ed Engl (2016) 55, 1680-1684. PubMed Europe PubMed DOI
Zhang,X., Bruning,J.B., George,J.H. and Abell,A.D. A mechanistic study on the inhibition of alpha-chymotrypsin by a macrocyclic peptidomimetic aldehyde Org Biomol Chem (2016) 14, 6970-6978. PubMed Europe PubMed DOI I

2015

Kurinomaru,T., Kameda,T. and Shiraki,K. Effects of multivalency and hydrophobicity of polyamines on enzyme hyperactivation of alpha-chymotrypsin J Mol Catal B Enzym (2015) 115, 135-139. DOI
Li,L.-P., Cai,X., Xiang,Y., Zhang,Y., Song,J., Yang,D.-C., Guan,Z. and He,Y.-H. The alpha-chymotrypsin-catalyzed Povarov reaction: one-pot synthesis of tetrahydroquinoline derivatives Green Chem (2015) 17, 3148-3156. DOI
Luong,T.Q. and Winter,R. Combined pressure and cosolvent effects on enzyme activity - a high-pressure stopped-flow kinetic study on alpha-chymotrypsin Phys Chem Chem Phys (2015) 17, 23273-23278. PubMed Europe PubMed DOI
Romero,C.M., Abella,J.S., Velazquez,A. and Sancho,J. Thermal denaturation of alpha-chymotrypsinogen A in presence of polyols at pH 2.0 and pH 3.0 J Therm Anal Calor (2015) 120, 489-499. DOI
Shibao,P.Y., Anjolette,F.A., Lopes,N.P. and Arantes,E.C. First serine protease inhibitor isolated from Rhinella schneideri poison J Venom Anim Toxins Incl Trop Dis (2015) 21, 30-30. PubMed Europe PubMed DOI PMC EPMC I
Sun,D., Jiang,G., Xie,Z. and Le,Z. alpha-chymotrypsin-catalyzed synthesis of bis(indolyl)alkanes in water Chin J Chem (2015) 33, 409-412. DOI
Uccello-Barretta,G., Balzano,F., Aiello,F., Vanni,L., Mori,M., Menta,S., Calcaterra,A. and Botta,B. Hydrolytic inhibition of alpha-chymotrypsin by 2,8,14,20-tetrakis(d-leucyl-d-valinamido)resorc[4]arenecarboxylic acid: a spectroscopic NMR and computational combined approach Org Biomol Chem (2015) 13, 916-924. PubMed Europe PubMed DOI I
Zhang,L., Xiao,X., Yuan,Y., Guo,Y., Li,M. and Pu,X. Probing immobilization mechanism of alpha-chymotrypsin onto carbon nanotube in organic media by molecular dynamics simulation Sci Rep (2015) 5, 9297-9297. PubMed Europe PubMed DOI
Zhang,X., Heng,S. and Abell,A.D. Photoregulation of alpha-chymotrypsin activity by spiropyran-based inhibitors in solution and attached to an optical fiber Chemistry (2015) 21, 10703-10713. PubMed Europe PubMed DOI I

2014

Chen,Q., Zhang,J., Zhao,K., Li,W., Miao,Q., Sun,Y., Zhao,X., Wei,T. and Yang,F. Lysosomal chymotrypsin induces mitochondrial fission in apoptotic cells by proteolytic activation of calcineurin Protein Cell (2014) 5, 643-647. PubMed Europe PubMed DOI
Cleary,J.A., Doherty,W., Evans,P. and Malthouse,J.P. Hemiacetal stabilization in a chymotrypsin inhibitor complex and the reactivity of the hydroxyl group of the catalytic serine residue of chymotrypsin Biochim Biophys Acta (2014) 1844, 1119-1127. PubMed Europe PubMed DOI I
Gupta,B.S., Taha,M. and Lee,M.-J. Superactivity of alpha-chymotrypsin with biological buffers, TRIS, TES, TAPS, and TAPSO in aqueous solutions RSC Adv (2014) 4, 51111-51116. DOI
Khan,Z.A., Afzal,N., Hussain,Z., Naqvi,S.A.R., Bari,A., Shahzad,S.A., Yar,M., Mahmood,N., Bukhari,S.A., Mansha,A., Zahoor,A.F., Khan,A.R. and Ahmad,M. Synthesis of 2-aryl-4H-3,1-benzoxazin-4-ones: a class of alpha-chymotrypsin inhibitors Asian J Chem (2014) 26, 4561-4565. DOI I
Latif,M.A., Tejo,B.A., Abedikargiban,R., Abdul Rahman,M.B. and Micaelo,N.M. Modeling stability and flexibility of alpha-chymotrypsin in room temperature ionic liquids J Biomol Struct Dyn (2014) 32, 1263-1273. PubMed Europe PubMed DOI
Murata,H., Cummings,C.S., Koepsel,R.R. and Russell,A.J. Rational tailoring of substrate and inhibitor affinity via ATRP polymer-based protein engineering Biomacromolecules (2014) 15, 2817-2823. PubMed Europe PubMed DOI
Perveen,S., Mustafa,S., Latif,M., Iqbal,L., Usmani,T.H., Khan,K.M. and Voelter,W. Unsymmetrical 1,3-disubstituted urea derivatives as alpha-chymotrypsin inhibitors Med Chem Res (2014) 23, 3585-3592. DOI I
Xie,Z.B., Sun,D.Z., Jiang,G.F. and Le,Z.G. Facile synthesis of bis(indolyl)methanes catalyzed by alpha-chymotrypsin Molecules (2014) 19, 19665-19677. PubMed Europe PubMed DOI

2013

Karna,N., Debowski,D., Gitlin,A., Legowska,A. and Rolka,K. Investigation of peptide splicing using two-peptide-chain analogs of trypsin inhibitor SFTI-1 FEBS J (2013) 280, 6213-6222. PubMed Europe PubMed DOI
Moyano,F., Setien,E., Silber,J.J. and Correa,N.M. Enzymatic hydrolysis of N-benzoyl-L-tyrosine p-nitroanilide by alpha-chymotrypsin in DMSO-water/AOT/n-heptane reverse micelles. A unique interfacial effect on the enzymatic activity Langmuir (2013) 29, 8245-8254. PubMed Europe PubMed DOI
Terentyeva,T.G., Hofkens,J., Komatsuzaki,T., Blank,K. and Li,C.B. Time-resolved single molecule fluorescence spectroscopy of an alpha-chymotrypsin catalyzed reaction J Phys Chem B (2013) 117, 1252-1260. PubMed Europe PubMed DOI
Vorob'ev,M.M. Quantification of two-step proteolysis model with consecutive demasking and hydrolysis of peptide bonds using casein hydrolysis by chymotrypsin Biochem Eng J (2013) 74, 60-68.
Yamashita,S., Sakabe,M., Ishizawa,T., Hasegawa,K., Urano,Y. and Kokudo,N. Visualization of the leakage of pancreatic juice using a chymotrypsin-activated fluorescent probe Br J Surg (2013) 100, 1220-1228. PubMed Europe PubMed DOI A

2012

Graf,L., Szilagyi,L. and Venekei,I. Chymotrypsin [ISSN:978-0-12-407742-3] (2012) 3, 2626-2633. DOI
Haerteis,S., Krappitz,M., Diakov,A., Krappitz,A., Rauh,R. and Korbmacher,C. Plasmin and chymotrypsin have distinct preferences for channel activating cleavage sites in the gamma subunit of the human epithelial sodium channel J Gen Physiol (2012) 140, 375-389. PubMed Europe PubMed DOI P
Hu,S.H., Kuo,C.H., Chang,C.M., Liu,Y.C., Chiang,W.D. and Shieh,C.J. Solvent selection and optimization of alpha-chymotrypsin-catalyzed synthesis of N-Ac-Phe-Tyr-NH2 using mixture design and response surface methodology Biotechnol Prog (2012) 28, 1443-1449. PubMed Europe PubMed DOI
Liuni,P., Jeganathan,A. and Wilson,D.J. Conformer selection and intensified dynamics during catalytic turnover in chymotrypsin Angew Chem Int Ed Engl (2012) 51, 9666-9669. PubMed Europe PubMed DOI
Petrillo,T., O'Donohoe,C.A., Howe,N. and Malthouse,J.P. Importance of tetrahedral intermediate formation in the catalytic mechanism of the serine proteases chymotrypsin and subtilisin Biochemistry (2012) 51, 6164-6170. PubMed Europe PubMed DOI I
Simon,L.M., Laczko,I., Demcsak,A., Toth,D., Kotorman,M. and Fulop,L. The formation of amyloid-like fibrils of alpha-chymotrypsin in different aqueous organic solvents Protein Pept Lett (2012) 19, 544-550. PubMed Europe PubMed DOI
Singh,S. and Sieburth,S.M. Serine protease inhibition by a silanediol peptidomimetic Org Lett (2012) 14, 4422-4425. PubMed Europe PubMed DOI I
Zhu,L., Yang,W., Meng,Y.Y., Xiao,X., Guo,Y., Pu,X. and Li,M. Effects of organic solvent and crystal water on gamma-chymotrypsin in acetonitrile media: observations from molecular dynamics simulation and DFT calculation J Phys Chem B (2012) 116, 3292-3304. PubMed Europe PubMed DOI

2011

Abuin,E., Lissi,E., Ahumada,M. and Calderon,C. Effect of human serum albumin on the kinetics of N-glutaryl-L-phenylalanine p-nitroanilide hydrolysis catalyzed by alpha-chymotrypsin Protein J (2011) 30, 143-147. PubMed Europe PubMed DOI
Brandt,N.N., Mankova,A.A. and Chikishev,A.Y. IR spectroscopy of structural changes of alpha-chymotrypsin related to the changes of function in organic solvents Moscow Univ Phys Bull (2011) 66, 282-285. DOI
Lauzon,C.B., van Zijl,P. and Stivers,J.T. Using the water signal to detect invisible exchanging protons in the catalytic triad of a serine protease J Biomol NMR (2011) 50, 299-314. PubMed Europe PubMed DOI PMC EPMC
Lazar,L.M., Fisher,S.Z., Moulin,A.G., Kovalevsky,A., Novak,W.R., Langan,P., Petsko,G.A. and Ringe,D. Time-of-flight neutron diffraction study of bovine gamma-chymotrypsin at the Protein Crystallography Station Acta Crystallogr Sect F Struct Biol Cryst Commun (2011) 67, 587-590. PubMed Europe PubMed DOI PMC EPMC S
Pastor,I., Vilaseca,E., Madurga,S., Garces,J.L., Cascante,M. and Mas,F. Effect of crowding by dextrans on the hydrolysis of N-succinyl-l-phenyl-Ala-p-nitroanilide catalyzed by alpha-chymotrypsin J Phys Chem B (2011) 115, 1115-1121. PubMed Europe PubMed DOI
Ruark,C.D., Hack,C.E., Robinson,P.J. and Gearhart,J.M. Quantitative structure-activity relationships for organophosphates binding to trypsin and chymotrypsin J Toxicol Environ Health A (2011) 74, 1-23. PubMed Europe PubMed DOI I
Terentyeva,T.G., Van Rossom,W., Van der Auweraer,M., Blank,K. and Hofkens,J. Morpholinecarbonyl-rhodamine 110 based substrates for the determination of protease activity with accurate kinetic parameters Bioconjug Chem (2011) 22, 1932-1938. PubMed Europe PubMed DOI A
Verma,P.K., Rakshit,S., Mitra,R.K. and Pal,S.K. Role of hydration on the functionality of a proteolytic enzyme alpha-chymotrypsin under crowded environment Biochimie (2011) 93, 1424-1433. PubMed Europe PubMed DOI

2010

Attri,P., Venkatesu,P. and Lee,M.J. Influence of osmolytes and denaturants on the structure and enzyme activity of alpha-chymotrypsin J Phys Chem B (2010) 114, 1471-1478. PubMed Europe PubMed DOI
Legowska,A., Debowski,D., Lesner,A., Wysocka,M. and Rolka,K. Selection of peptomeric inhibitors of bovine alpha-chymotrypsin and cathepsin G based on trypsin inhibitor SFTI-1 using a combinatorial chemistry approach Mol Divers (2010) 14, 51-58. PubMed Europe PubMed DOI I
Qasim,M.A., Song,J., Markley,J.L. and Laskowski,M., Jr. Cleavage of peptide bonds bearing ionizable amino acids at P(1) by serine proteases with hydrophobic S(1) pocket Biochem Biophys Res Commun (2010) 400, 507-510. PubMed Europe PubMed DOI P

2009

Chen,A.Y., Jani,A.S., Zheng,L., Burke,P.J. and Brody,J.P. Microfabricated arrays of cylindrical wells facilitate single-molecule enzymology of alpha-chymotrypsin Biotechnol Prog (2009) 25, 929-937. PubMed Europe PubMed DOI A
Lesner,A., Wysocka,M., Solek,M., Legowska,A. and Rolka,K. Low-molecular-weight aldehyde inhibitors of cathepsin G Protein Pept Lett (2009) 16, 408-410. PubMed Europe PubMed DOI I
Mino,G. and Contreras,R. On the role of short and strong hydrogen bonds on the mechanism of action of a model chymotrypsine active site J Phys Chem A (2009) 113, 5769-5772. PubMed Europe PubMed DOI
Novak,W.R., Moulin,A.G., Blakeley,M.P., Schlichting,I., Petsko,G.A. and Ringe,D. A preliminary neutron diffraction study of gamma-chymotrypsin Acta Crystallograph Sect F Struct Biol Cryst Commun (2009) 65, 317-320. PubMed Europe PubMed DOI PMC EPMC S
Pietrusewicz,E., Sienczyk,M. and Oleksyszyn,J. Novel diphenyl esters of peptidyl alpha-aminoalkylphosphonates as inhibitors of chymotrypsin and subtilisin J Enzyme Inhib Med Chem (2009) 24, 1229-1236. PubMed Europe PubMed DOI I

2008

Banerjee,D. and Pal,S.K. Conformational Dynamics at the Active Site of alpha-Chymotrypsin and Enzymatic Activity Langmuir (2008) 24, 8163-8168. PubMed Europe PubMed DOI
Khan,K.M., Mughal,U.R., Lodhi,M.A. and Choudhary,M.I. Synthesis and chymotrypsin inhibitory activity of substituted oxazolones Lett Drug Des Discov (2008) 5, 52-56. DOI I
Linington,R.G., Edwards,D.J., Shuman,C.F., McPhail,K.L., Matainaho,T. and Gerwick,W.H. Symplocamide A, a potent cytotoxin and chymotrypsin inhibitor from the marine cyanobacterium Symploca sp J Nat Prod (2008) 71, 22-27. PubMed Europe PubMed DOI I
Pearson,D., Alexander,N. and Abell,A.D. Improved photocontrol of alpha-chymotrypsin activity: peptidomimetic trifluoromethylketone photoswitch enzyme inhibitors Chemistry (2008) 14, 7358-7365. PubMed Europe PubMed DOI I
Shokhen,M., Khazanov,N. and Albeck,A. Screening of the active site from water by the incoming ligand triggers catalysis and inhibition in serine proteases Proteins (2008) 70, 1578-1587. PubMed Europe PubMed DOI
Wysocka,M., Lesner,A., Legowska,A., Jaskiewicz,A., Miecznikowska,H. and Rolka,K. Designing of substrates and inhibitors of bovine alpha-chymotrypsin with synthetic phenylalanine analogues in position P(1) Protein Pept Lett (2008) 15, 260-264. PubMed Europe PubMed DOI

2007

Baumann,H.I., Keller,S., Wolter,F.E., Nicholson,G.J., Jung,G., Sussmuth,R.D. and Juttner,F. Planktocyclin, a cyclooctapeptide protease inhibitor produced by the freshwater cyanobacterium Planktothrix rubescens J Nat Prod (2007) 70, 1611-1615. PubMed Europe PubMed DOI I
Bruylants,G., Redfield,C. and Bartik,K. Developments in the characterisation of the catalytic triad of alpha-chymotrypsin: effect of the protonation state of Asp102 on the 1H NMR signals of His57 Chembiochem (2007) 8, 51-54. PubMed Europe PubMed DOI
Moulin,A., Bell,J.H., Pratt,R.F. and Ringe,D. Inhibition of chymotrypsin by a complex of ortho-vanadate and benzohydroxamic acid: structure of the inert complex and its mechanistic interpretation Biochemistry (2007) 46, 5982-5990. PubMed Europe PubMed DOI PMC EPMC I
Spink,E., Cosgrove,S., Rogers,L., Hewage,C. and Malthouse,J.P. 13C and 1H NMR studies of ionizations and hydrogen bonding in chymotrypsin-glyoxal inhibitor complexes J Biol Chem (2007) 282, 7852-7861. PubMed Europe PubMed DOI I
Spink,E., Hewage,C. and Malthouse,J.P. Determination of the structure of tetrahedral transition state analogues bound at the active site of chymotrypsin using 18O and 2H isotope shifts in the 13C NMR spectra of glyoxal inhibitors Biochemistry (2007) 46, 12868-12874. PubMed Europe PubMed DOI S I
Wysocka,M., Kwiatkowska,B., Rzadkiewicz,M., Lesner,A. and Rolka,K. Selection of new chromogenic substrates of serine proteinases using combinatorial chemistry methods Comb Chem High Throughput Screen (2007) 10, 171-180. PubMed Europe PubMed DOI A

2006

[YEAR:15-12-2006]Hudaky,P. and Perczel,A. A self-stabilized model of the chymotrypsin catalytic pocket. The energy profile of the overall catalytic cycle Proteins (15-12-2006) 62, 749-759. PubMed Europe PubMed DOI
Shibli,A., Smoum,R., Goldberg,I., Rubinstein,A. and Srebnik,M. alpha-Amino boronates as cyanoborane complexes: crystal structure and inhibition properties for the serine proteases: alpha-chymotrypsin and trypsin Appl Organomet Chem (2006) 20, 459-462. DOI S I
Sola,R.J. and Griebenow,K. Influence of modulated structural dynamics on the kinetics of alpha-chymotrypsin catalysis. Insights through chemical glycosylation, molecular dynamics and domain motion analysis FEBS J (2006) 273, 5303-5319. PubMed Europe PubMed DOI

2005

[YEAR:15-3-2005]Abuin,E., Lissi,E. and Duarte,R. Kinetics of N-glutaryl-L-phenylalanine p-nitroanilide hydrolysis catalyzed by alpha-chymotrypsin in aqueous solutions of dodecyltrimethylammonium bromide J Colloid Interface Sci (15-3-2005) 283, 539-543. PubMed Europe PubMed DOI
[YEAR:7-1-2005]Segev,O., Columbus,I., Ashani,Y. and Cohen,Y. Probing the molecular interaction of chymotrypsin with organophosphorus compounds by 31P diffusion NMR in aqueous solutions J Org Chem (7-1-2005) 70, 309-314. PubMed Europe PubMed DOI I
Singh,N., Jabeen,T., Sharma,S., Roy,I., Gupta,M.N., Bilgrami,S., Somvanshi,R.K., Dey,S., Perbandt,M., Betzel,C., Srinivasan,A. and Singh,T.P. Detection of native peptides as potent inhibitors of enzymes. Crystal structure of the complex formed between treated bovine alpha-chymotrypsin and an autocatalytically produced fragment, IIe-Val-Asn-Gly-Glu-Glu-Ala-Val-Pro-Gly-Ser-Trp-Pro-Trp, at 2.2 angstroms resolution FEBS J (2005) 272, 562-572. PubMed Europe PubMed DOI S I

2004

[YEAR:3-12-2004]Czapinska,H., Helland,R., Smalas,A.O. and Otlewski,J. Crystal structures of five bovine chymotrypsin complexes with P1 BPTI variants J Mol Biol (3-12-2004) 344, 1005-1020. PubMed Europe PubMed DOI S
Graf,L., Szilagyi,L. and Venekei,I. Chymotrypsin [ISSN:0-12-079610-4] (2004) 2, 1495-1501. V
[YEAR:27-1-2004]Hengge,A.C. and Stein,R.L. Role of protein conformational mobility in enzyme catalysis: acylation of alpha-chymotrypsin by specific peptide substrates Biochemistry (27-1-2004) 43, 742-747. PubMed Europe PubMed DOI
[YEAR:2-11-2004]Radisky,E.S., Kwan,G., Karen Lu,C.J. and Koshland,D.E., Jr. Binding, proteolytic, and crystallographic analyses of mutations at the protease-inhibitor interface of the subtilisin BPN'/chymotrypsin inhibitor 2 complex Biochemistry (2-11-2004) 43, 13648-13656. PubMed Europe PubMed DOI S
[YEAR:3-5-2004]Roy,I. and Gupta,M.N. Preparation of highly active alpha-chymotrypsin for catalysis in organic media Bioorg Med Chem Lett (3-5-2004) 14, 2191-2193. PubMed Europe PubMed DOI
Verheyden,G., Matrai,J., Volckaert,G. and Engelborghs,Y. A fluorescence stopped-flow kinetic study of the conformational activation of alpha-chymotrypsin and several mutants Protein Sci (2004) 13, 2533-2540. PubMed Europe PubMed DOI PMC EPMC

2003

[YEAR:25-3-2003]Case,A. and Stein,R.L. Mechanistic origins of the substrate selectivity of serine proteases Biochemistry (25-3-2003) 42, 3335-3348. PubMed Europe PubMed DOI
[YEAR:31-10-2003]Helland,R., Czapinska,H., Leiros,I., Olufsen,M., Otlewski,J. and Smalas,A.O. Structural consequences of accommodation of four non-cognate amino acid residues in the S1 pocket of bovine trypsin and chymotrypsin J Mol Biol (31-10-2003) 333, 845-861. PubMed Europe PubMed DOI

2002

[YEAR:1-3-2002]Djurdjevic-Pahl,A., Hewage,C. and Malthouse,J.P. 13C-NMR study of the inhibition of delta-chymotrypsin by a tripeptide-glyoxal inhibitor Biochem J (1-3-2002) 362, 339-347. PubMed Europe PubMed DOI PMC EPMC I
[YEAR:8-4-2002]Wouters,J., Huygens,M., Pochet,L., Pirotte,B., Durant,F. and Masereel,B. Structural approach of the mechanism of inhibition of alpha-chymotrypsin by coumarins Bioorg Med Chem Lett (8-4-2002) 12, 1109-1112. PubMed Europe PubMed DOI I

2001

[YEAR:9-7-2001]Aoyama,Y., Uenaka,M., Konoike,T., Hayasaki-Kajiwara,Y., Naya,N. and Nakajima,M. Inhibition of serine proteases: activity of 1,3-diazetidine-2,4-diones Bioorg Med Chem Lett (9-7-2001) 11, 1691-1694. PubMed Europe PubMed DOI I
Bodi,A., Kaslik,G., Venekei,I. and Graf,L. Structural determinants of the half-life and cleavage site preference in the autolytic inactivation of chymotrypsin Eur J Biochem (2001) 268, 6238-6246. PubMed Europe PubMed DOI
[YEAR:30-11-2001]Ghani,U., Ng,K.K.S., Atta,u.R., Choudhary,M.I., Ullah,N. and James,M.N.G. Crystal structure of gamma-chymotrypsin in complex with 7-hydroxycoumarin J Mol Biol (30-11-2001) 314, 519-525. PubMed Europe PubMed DOI S I
[YEAR:27-2-2001]Neidhart,D., Wei,Y., Cassidy,C., Lin,J., Cleland,W.W. and Frey,P.A. Correlation of low-barrier hydrogen bonding and oxyanion binding in transition state analogue complexes of chymotrypsin Biochemistry (27-2-2001) 40, 2439-2447. PubMed Europe PubMed DOI
Spreti,N., Di Profio,P., Marte,L., Bufali,S., Brinchi,L. and Savelli,G. Activation and stabilization of alpha-chymotrypsin by cationic additives Eur J Biochem (2001) 268, 6491-6497. PubMed Europe PubMed DOI

2000

Kitson,T.M. and Freeman,G.H. 3,4-Dihydro-3,4,6-trimethyl-2H,8H-pyrano-[3,2g]-1,3-benzoxazin-2,8-dione, a potential fluorogenic reporter group reagent for esterases-synthesis and interaction with chymotrypsin Bioorg Chem (2000) 28, 273-282. PubMed Europe PubMed DOI I
Mac Sweeney,A., Birrane,G., Walsh,M.A., O'Connell,T., Malthouse,J.P. and Higgins,T.M. Crystal structure of delta-chymotrypsin bound to a peptidyl chloromethyl ketone inhibitor Acta Crystallogr D Biol Crystallogr (2000) 56, 280-286. PubMed Europe PubMed DOI S I
Pochet,L., Doucet,C., Dive,G., Wouters,J., Masereel,B., Reboud-Ravaux,M. and Pirotte,B. Coumarinic derivatives as mechanism-based inhibitors of alpha-chymotrypsin and human leukocyte elastase Bioorg Med Chem (2000) 8, 1489-1501. PubMed Europe PubMed DOI I

1999

[YEAR:19-5-1999]Bao,D.H., Huskey,W.P., Kettner,C.A. and Jordan,F. Hydrogen bonding to active-site histidine in peptidyl boronic acid inhibitor complexes of chymotrypsin and subtilisin: proton magnetic resonance assignments and H D fractionation J Am Chem Soc (19-5-1999) 121, 4684-4689. DOI I
Bianco,A., Kaiser,D. and Jung,G. N-Hydroxy peptides as substrates for alpha-chymotrypsin J Pept Res (1999) 54, 544-548. PubMed Europe PubMed DOI A
Hudaky,P., Kaslik,G., Venekei,I. and Graf,L. The differential specificity of chymotrypsin A and B is determined by amino acid 226 Eur J Biochem (1999) 259, 528-533. PubMed Europe PubMed DOI
[YEAR:10-12-1999]Janes,L.E., Cimpoia,A. and Kazlauskas,R.J. Protease-mediated separation of cis and trans diastereomers of 2(R,S)-benzyloxymethyl-4(S)-carboxylic acid 1,3-dioxolane methyl ester: Intermediates for the synthesis of dioxolane nucleosides J Org Chem (10-12-1999) 64, 9019-9029.

1998

Kashima,A., Inoue,Y., Sugio,S., Maeda,I., Nose,T. and Shimohigashi,Y. X-ray crystal structure of a dipeptide-chymotrypsin complex in an inhibitory interaction Eur J Biochem (1998) 255, 12-23. PubMed Europe PubMed S I
[YEAR:25-8-1998]Lin,J., Cassidy,C.S. and Frey,P.A. Correlations of the basicity of His 57 with transition state analogue binding, substrate reactivity, and the strength of the low-barrier hydrogen bond in chymotrypsin Biochemistry (25-8-1998) 37, 11940-11948. PubMed Europe PubMed DOI
[YEAR:15-3-1998]Rafiq,A. and Bailey,G. A more sensitive Hummel assay for chymotrypsin Anal Biochem (15-3-1998) 257, 233-234. PubMed Europe PubMed DOI A
Stoll,V.S., Eger,B.T., Hynes,R.C., Martichonok,V., Jones,J.B. and Pai,E.F. Differences in binding modes of enantiomers of 1-acetamido boronic acid based protease inhibitors: crystal structures of gamma-chymotrypsin and subtilisin Carlsberg complexes Biochemistry (1998) 37, 451-462. PubMed Europe PubMed DOI S I

1997

Capasso,C., Rizzi,M., Menegatti,E., Ascenzi,P. and Bolognesi,M. Crystal structure of the bovine alpha-chymotrypsin:Kunitz inhibitor complex. An example of multiple protein:protein recognition sites J Mol Recognit (1997) 10, 26-35. PubMed Europe PubMed DOI S
[YEAR:14-11-1997]Galunsky,B., Ignatova,S. and Kasche,V. Temperature effects on S1- and S'1-enantioselectivity of alpha-chymotrypsin Biochim Biophys Acta (14-11-1997) 1343, 130-138. PubMed Europe PubMed DOI
[YEAR:26-11-1997]Ito,K., Igarashi,K., Muramatsu,M., Harada,T., Hayashi,Y., Katada,J. and Uno,I. Potent inactivator of alpha-chymotrypsin: 2,2-dimethyl-3-(N-4-cyanobenzoyl)amino-5-phenyl pentanoic anhydride Biochem Biophys Res Commun (26-11-1997) 240, 850-855. PubMed Europe PubMed DOI
Kurth,T., Ullmann,D., Jakubke,H.D. and Hedstrom,L. Converting trypsin to chymotrypsin: Structural determinants of S1' specificity Biochemistry (1997) 36, 10098-10104. PubMed Europe PubMed DOI
Takahashi,K. Restriction of the specificities of trypsin and alpha-chymotrypsin at a high alkaline pH value Protein Pept Lett (1997) 4, 33-38.
Varallyay,E., Lengyel,Z., Graf,L. and Szilagyi,L. The role of disulfide bond C191-C220 in trypsin and chymotrypsin Biochem Biophys Res Commun (1997) 230, 592-596. PubMed Europe PubMed DOI

1996

Kitson,T.M. Comparison of resorufin acetate and p-nitrophenyl acetate as substrates for chymotrypsin Bioorg Chem (1996) 24, 331-339. A

1995

Rodgers,K.J., Melrose,J. and Ghosh,P. Biotin-labeled potato chymotrypsin inhibitor-1: a useful probe for the detection and quantitation of chymotrypsin-like serine proteinases on Western blots and its application in the detection of a serine proteinase synthesised by articular chondrocytes Anal Biochem (1995) 227, 129-134. PubMed Europe PubMed DOI A

1994

[YEAR:26-7-1994]Hedstrom,L., Perona,J.J. and Rutter,W.J. Converting trypsin to chymotrypsin: residue 172 is a substrate specificity determinant Biochemistry (26-7-1994) 33, 8757-8763. PubMed Europe PubMed DOI
[YEAR:26-7-1994]Hedstrom,L., Farr-Jones,S., Kettner,C.A. and Rutter,W.J. Converting trypsin to chymotrypsin: ground-state binding does not determine substrate specificity Biochemistry (26-7-1994) 33, 8764-8769. PubMed Europe PubMed DOI I
[YEAR:22-11-1994]Kreutter,K., Steinmetz,A.C., Liang,T.C., Prorok,M., Abeles,R.H. and Ringe,D. Three-dimensional structure of chymotrypsin inactivated with (2S)-N-acetyl-L-alanyl-L-phenylalanyl alpha-chloroethane: implications for the mechanism of inactivation of serine proteases by chloroketones Biochemistry (22-11-1994) 33, 13792-13800. PubMed Europe PubMed DOI S I
[YEAR:16-8-1994]Prorok,M., Albeck,A., Foxman,B.M. and Abeles,R.H. Chloroketone hydrolysis by chymotrypsin and N-methylhistidyl-57-chymotrypsin: implications for the mechanism of chymotrypsin inactivation by chloroketones Biochemistry (16-8-1994) 33, 9784-9790. PubMed Europe PubMed I
[YEAR:12-4-1994]Schellenberger,V., Turck,C.W. and Rutter,W.J. Role of the S' subsites in serine protease catalysis. Active-site mapping of rat chymotrypsin, rat trypsin, alpha-lytic protease, and cercarial protease from Schistosoma mansoni Biochemistry (12-4-1994) 33, 4251-4257. PubMed Europe PubMed
[YEAR:14-6-1994]Yennawar,N.H., Yennawar,H.P. and Farber,G.K. X-ray crystal structure of gamma-chymotrypsin in hexane Biochemistry (14-6-1994) 33, 7326-7336. PubMed Europe PubMed S

1993

Norin,M., Hult,K., Mattson,A. and Norin,T. Molecular modelling of chymotrypsin-substrate interactions: Calculation of enantioselectivity Biocatal Biotransform (1993) 7, 131-147. DOI P

1992

[YEAR:5-5-1992]Frigerio,F., Coda,A., Pugliese,L., Lionetti,C., Menegatti,E., Amiconi,G., Schnebli,H.P., Ascenzi,P. and Bolognesi,M. Crystal and molecular structure of the bovine alpha-chymotrypsin-eglin c complex at 2.0 A resolution J Mol Biol (5-5-1992) 225, 107-123. PubMed Europe PubMed DOI S
[YEAR:6-3-1992]Hedstrom,L., Szilagyi,L. and Rutter,W.J. Converting trypsin to chymotrypsin: the role of surface loops Science (6-3-1992) 255, 1249-1253. PubMed Europe PubMed

1991

Harel,M., Su,C.T., Frolow,F., Silman,I. and Sussman,J.L. Gamma-chymotrypsin is a complex of alpha-chymotrypsin with its own autolysis products Biochemistry (1991) 30, 5217-5225. PubMed Europe PubMed
Siezen,R.J., de Vos,W.M., Leunissen,J.A. and Dijkstra,B.W. Homology modelling and protein engineering strategy of subtilases, the family of subtilisin-like serine proteinases Protein Eng (1991) 4, 719-737. PubMed Europe PubMed

1990

Bolognesi,M., Pugliese,L., Gatti,G., Frigerio,F., Coda,A., Antolini,L., Schnebli,H.P., Menegatti,E., Amiconi,G. and Ascenzi,P. X-ray crystal structure of the bovine alpha-chymotrypsin/eglin c complex at 2.6 A resolution J Mol Recognit (1990) 3, 163-168. PubMed Europe PubMed DOI S
Okada,Y., Tsuboi,S., Tsuda,Y., Nagamatsu,Y. and Yamamoto,J. Synthesis of a trihexacontapeptide corresponding to the sequence 8-70 of eglin c and studies on the relationship between the structure and the inhibitory activity against human leukocyte elastase, cathepsin G and alpha-chymotrypsin FEBS Lett (1990) 272, 113-116. PubMed Europe PubMed DOI
Schmitt,S., Goetschel,J.D., Rommelt,E.M. and Adler,C. One-step purification of trypsin and alpha-chymotrypsin by affinity chromatography on Eupergit-aprotinin, a novel carrier for purification of serine proteases J Chromatogr (1990) 510, 239-242. PubMed Europe PubMed DOI

1987

[YEAR:20-5-1987]Fujinaga,M., Sielecki,A.R., Read,R.J., Ardelt,W., Laskowski,M., Jr. and James,M.N. Crystal and molecular structures of the complex of alpha-chymotrypsin with its inhibitor turkey ovomucoid third domain at 1.8 A resolution J Mol Biol (20-5-1987) 195, 397-418. PubMed Europe PubMed S
Stein,R.L. and Strimpler,A.M. P1 residue determines the operation of the catalytic triad of serine proteases during hydrolyses of acyl-enzymes J Am Chem Soc (1987) 109, 4387-4390.
Tulinsky,A. and Blevins,R.A. Structure of a tetrahedral transition state complex of alpha-chymotrypsin dimer at 1.8-A resolution J Biol Chem (1987) 262, 7737-7743. PubMed Europe PubMed S I

1986

[YEAR:25-3-1986]Daniels,S.B. and Katzenellenbogen,J.A. Halo enol lactones: studies on the mechanism of inactivation of alpha-chymotrypsin Biochemistry (25-3-1986) 25, 1436-1444. PubMed Europe PubMed I
Groutas,W.C., Giri,P.K., Crowley,J.P., Castrisos,J.C. and Brubaker,M.J. The Lossen rearrangement in biological systems. Inactivation of leukocyte elastase and alpha-chymotrypsin by (dl)-3-benzyl-N- (methanesulfonyloxy) succinimide Biochem Biophys Res Commun (1986) 141, 741-748. PubMed Europe PubMed I

1985

Blevins,R.A. and Tulinsky,A. The refinement and the structure of the dimer of alpha-chymotrypsin at 1.67-A resolution J Biol Chem (1985) 260, 4264-4275. PubMed Europe PubMed S
Church,F.C., Noyes,C.M. and Griffith,M.J. Inhibition of chymotrypsin by heparin cofactor II Proc Natl Acad Sci U S A (1985) 82, 6431-6434. PubMed Europe PubMed PMC EPMC
[YEAR:9-4-1985]Hemmi,K., Harper,J.W. and Powers,J.C. Inhibition of human leukocyte elastase, cathepsin G, chymotrypsin A alpha, and porcine pancreatic elastase with substituted isobenzofuranones and benzopyrandiones Biochemistry (9-4-1985) 24, 1841-1848. PubMed Europe PubMed I
[YEAR:1-1-1985]Ringe,D., Seaton,B.A., Gelb,M.H. and Abeles,R.H. Inactivation of chymotrypsin by 5-benzyl-6-chloro-2-pyrone: 13C NMR and X-ray diffraction analyses of the inactivator-enzyme complex Biochemistry (1-1-1985) 24, 64-68. PubMed Europe PubMed S I
Tsukada,H. and Blow,D.M. Structure of alpha-chymotrypsin refined at 1.68 A resolution J Mol Biol (1985) 184, 703-711. PubMed Europe PubMed S
[YEAR:5-10-1985]Wang,D., Bode,W. and Huber,R. Bovine chymotrypsinogen A X-ray crystal structure analysis and refinement of a new crystal form at 1.8 A resolution J Mol Biol (5-10-1985) 185, 595-624. PubMed Europe PubMed S

1984

[YEAR:23-11-1984]Fischer,G., Bang,H., Berger,E. and Schellenberger,A. Conformational specificity of chymotrypsin toward proline-containing substrates Biochim Biophys Acta (23-11-1984) 791, 87-97. PubMed Europe PubMed DOI
[YEAR:18-12-1984]Gelb,M.H. and Abeles,R.H. Mechanism of inactivation of chymotrypsin by 3-benzyl-6-chloro-2-pyrone Biochemistry (18-12-1984) 23, 6596-6604. PubMed Europe PubMed I
[YEAR:10-4-1984]Hedstrom,L., Moorman,A.R., Dobbs,J. and Abeles,R.H. Suicide inactivation of chymotrypsin by benzoxazinones Biochemistry (10-4-1984) 23, 1753-1759. PubMed Europe PubMed I
Lambros,S.A., Richards,W.G. and Marchington,A.F. Theoretical calculations on enzyme-substrate interactions: the binding of n-alkylboronic acids to alpha-chymotrypsin A Theochem (1984) 109, 61-71. I
[YEAR:1-6-1984]Lijnen,H.R., Uytterhoeven,M. and Collen,D. Inhibition of trypsin-like serine proteinases by tripeptide arginyl and lysyl chloromethylketones Thromb Res (1-6-1984) 34, 431-437. PubMed Europe PubMed I

1983

[YEAR:25-12-1983]Daniels,S.B., Cooney,E., Sofia,M.J., Chakravarty,P.K. and Katzenellenbogen,J.A. Haloenol lactones. Potent enzyme-activated irreversible inhibitors for alpha-chymotrypsin J Biol Chem (25-12-1983) 258, 15046-15053. PubMed Europe PubMed I
Harper,J.W., Hemmi,K. and Powers,J.C. New mechanism-based serine protease inhibitors: inhibition of human leukocyte elastase, porcine pancreatic elastase, human leukocyte cathepsin G, and chymotrypsin by 3-chloroisocoumarin and 3,3-dichlorophthatide J Am Chem Soc (1983) 105, 6518-6520. I
Oksenoit,E.S., Petrova,E.N., Filippova,I.Y., Lavrenova,G.I., Tarasova,N.I. and Stepanov,V.M. A chromogenic substrate of alpha-chymotrypsin - the p-nitroanilide of L-pyroglutamyl-L-phenylalanine Chem Nat Compd (1983) 19, 126- DOI A
Shah,D.O. and Gorenstein,D.G. Fluorine nuclear magnetic resonance spectroscopy of "transition state analogue" complexes of the D and L enantiomers of N-acetyl-p-fluorophenylalaninal and alpha-chymotrypsin Biochemistry (1983) 22, 6096-6101.
Wipff,G., Dearing,A., Weiner,P.K., Blaney,J.M. and Kollman,P.A. Molecular mechanics studies of enzyme-substrate interactions: the interaction of L- and D-N-acetyltryptophanamide with alpha-chymotrypsin J Am Chem Soc (1983) 105, 997-1005.

1982

Lobermann,H., Lottspeich,F., Bode,W. and Huber,R. Interaction of human alpha1-proteinase inhibitor with chymotrypsinogen A and crystallization of a proteolytically modified alpha1-proteinase inhibitor Hoppe Seylers Z Physiol Chem (1982) 363, 1377-1388. PubMed Europe PubMed
[YEAR:10-5-1982]Yoshimura,T., Barker,L.N. and Powers,J.C. Specificity and reactivity of human leukocyte elastase, porcine pancreatic elastase, human granulocyte cathepsin G, and bovine pancreatic chymotrypsin with arylsulfonyl fluorides. Discovery of a new series of potent and specific irreversible elastase inhibitors J Biol Chem (10-5-1982) 257, 5077-5084. PubMed Europe PubMed I

1981

Hamilton,S.E. and Zerner,B. Solvent effects on the deacylation of acyl-chymotrypsins: a critical comment on the charge-relay hypothesis J Am Chem Soc (1981) 103, 1827-1831.
Shibata,M., Kieber-Emmons,T., Dutta,S. and Rein,R. A comparative analysis of the stability of the chymotrypsin charge triad Int J Quantum Chem (1981) 20, 409-417. DOI

1980

Brodrick,J.W., Glaser,C.B., Largman,C., Geokas,M.C., Graceffo,M., Fassett,M. and Maeda,H. Interaction of chymotrypsinogens with alpha1-protease inhibitor Biochemistry (1980) 19, 4865-4870. PubMed Europe PubMed

1979

DelMar,E.G., Largman,C., Brodrick,J.W. and Geokas,M.C. A sensitive new substrate for chymotrypsin Anal Biochem (1979) 99, 316-320. PubMed Europe PubMed
[YEAR:28-8-1979]Watanabe,H., Green,G.D. and Shaw,E. A comparison of the behavior of chymotrypsin and cathepsin B towards peptidyl diazomethyl ketones Biochem Biophys Res Commun (28-8-1979) 89, 1354-1360. PubMed Europe PubMed I

1978

Bloom,J.W. and Hunter,M.J. Interactions of alpha1-antitrypsin with trypsin and chymotrypsin J Biol Chem (1978) 253, 547-559. PubMed Europe PubMed
Lively,M.O. and Powers,J.C. Specificity and reactivity of human granulocyte elastase and cathepsin G, porcine pancreatic elastase, bovine chymotrypsin and trypsin toward inhibition with sulfonyl fluorides Biochim Biophys Acta (1978) 525, 171-179. PubMed Europe PubMed I

1977

Powers,J.C. and Gupton,B.F. Reaction of serine proteases with aza-amino acid and aza-peptide derivatives Methods Enzymol (1977) 46, 208-216. PubMed Europe PubMed DOI V I

1976

[YEAR:5-10-1976]Birktoft,J.J., Kraut,J. and Freer,S.T. A detailed structural comparison between the charge relay system in chymotrypsinogen and in alpha-chymotrypsin Biochemistry (5-10-1976) 15, 4481-4485. PubMed Europe PubMed
Blow,D.M. Structure and mechanism of chymotrypsin Acc Chem Res (1976) 9, 145-152.

1975

Rao,K.N. and Lombardi,B. Substrate solubilization for the Hummel alpha-chymotrypsin assay Anal Biochem (1975) 65, 548-551. PubMed Europe PubMed A

1974

[YEAR:5-7-1974]Robillard,G. and Shulman,R.G. High resolution nuclear magnetic resonance studies of the active site of chymotrypsin. II. Polarization of histidine 57 by substrate analogues and competitive inhibitors J Mol Biol (5-7-1974) 86, 541-558. PubMed Europe PubMed
Tomlinson,G., Shaw,M.C. and Viswanatha,T. Affinity chromatography of chymotrypsin(s) Methods Enzymol (1974) 34, 415-420. PubMed Europe PubMed DOI

1973

[YEAR:25-2-1973]Fersht,A.R. and Sperling,J. The charge relay system in chymotrypsin and chymotrypsinogen J Mol Biol (25-2-1973) 74, 137-149. PubMed Europe PubMed
Jameson,G.W., Roberts,D.V., Adams,R.W., Kyle,W.S. and Elmore,D.T. Determination of the operational molarity of solutions of bovine alpha-chymotrypsin, trypsin, thrombin and factor Xa by spectrofluorimetric titration Biochem J (1973) 131, 107-117. PubMed Europe PubMed PMC EPMC I
Kurachi,K., Powers,J.C. and Wilcox,P.E. Kinetics of the reaction of chymotrypsin A-alpha with peptide chloromethyl ketones in relation to its subsite specificity Biochemistry (1973) 12, 771-777. PubMed Europe PubMed I
Tatsuta,K., Mikami,N., Fujimoto,K., Umezawa,S. and Umezawa,H. The structure of chymostatin, a chymotrypsin inhibitor J Antibiot (Tokyo) (1973) 26, 625-646. PubMed Europe PubMed I

1972

Ako,H., Ryan,C.A. and Foster,R.J. The purification by affinity chromatography of a proteinase inhibitor binding species of anhydrochymotrypsin Biochem Biophys Res Commun (1972) 46, 1639-1645. PubMed Europe PubMed
Melville,J.C. and Ryan,C.A. Chymotrypsin inhibitor I from potatoes. Large scale preparation and characterization of its subunit components J Biol Chem (1972) 247, 3445-3453. PubMed Europe PubMed

1971

Blow,D.M. 6. The structure of chymotrypsin Enzymes (1971) 3, 185-212. DOI S
Kraut,J. 7. Chymotrypsin: chemical properties and catalysis Enzymes (1971) 3, 213-248. DOI
Segal,D.M., Powers,J.C., Cohen,G.H., Davies,D.R. and Wilcox,P.E. Substrate binding site in bovine chymotrypsin A-gamma. A crystallographic study using peptide chloromethylketones as site-specific inhibitors Biochemistry (1971) 10, 3728-3738. PubMed Europe PubMed S I
[YEAR:27-4-1971]Uren,J.R. Affinity chromatography of proteolytic enzymes Biochim Biophys Acta (27-4-1971) 236, 67-73. PubMed Europe PubMed
Wunsch,E., Hogel-Betz,A. and Jaeger,E. [Chromophoric substrates. IV. A new substrate for the quantitative determination of chymotrypsin A] HOPPE-SEYLERS'S Z PHYSIOL CHEM (1971) 352, 1553-1559. PubMed Europe PubMed DOI A

1970

Freer,S.T., Kraut,J., Robertus,J.D., Wright,H.T. and Xuong,N.H. Chymotrypsinogen: 2.5-angstrom crystal structure, comparison with alpha-chymotrypsin, and implications for zymogen activation Biochemistry (1970) 9, 1997-2009. PubMed Europe PubMed S
[YEAR:14-12-1970]Henderson,R. Structure of crystalline alpha-chymotrypsin. IV. The structure of indoleacryloyl-alpha-chymotrypsin and its relevance to the hydrolytic mechanism of the enzyme J Mol Biol (14-12-1970) 54, 341-354. PubMed Europe PubMed
Melville,J.C. and Ryan,C.A. Chymotrypsin inhibitor I from potatoes; a multisite inhibitor composed of subunits Arch Biochem Biophys (1970) 138, 700-702. PubMed Europe PubMed
Umezawa,H., Aoyagi,T., Morishima,H., Kunimoto,S., Matsuzaki,M., Hamada,M. and Takeuchi,T. Chymostatin, a new chymotrypsin inhibitor produced by actinomycetes J Antibiot (Tokyo) (1970) 23, 425-427. PubMed Europe PubMed I
Wilcox,P.E. Chymotrypsinogens - chymotrypsins Methods Enzymol (1970) 19, 64-108. DOI V

1969

Blow,D.M., Birktoft,J.J. and Hartley,B.S. Role of a buried acid group in the mechanism of action of chymotrypsin Nature (1969) 221, 337-340. PubMed Europe PubMed DOI
Brot,F.E. and Bender,M.L. Use of the specificity constant of alpha-chymotrypsin J Am Chem Soc (1969) 91, 7187-7197.
[YEAR:4-2-1969]Gratecos,D., Guy,O., Rovery,M. and Desnuelle,P. On the two anionic chymotrypsinogens of porcine pancreas Biochim Biophys Acta (4-2-1969) 175, 82-96. PubMed Europe PubMed
Steitz,T.A., Henderson,R. and Blow,D.M. Structure of crystalline alpha-chymotrypsin. 3. Crystallographic studies of substrates and inhibitors bound to the active site of alpha-chymotrypsin J Mol Biol (1969) 46, 337-348. PubMed Europe PubMed S

1968

Elmore,D.T. and Smyth,J.J. A new method for determining the absolute molarity of solutions of trypsin and chymotrypsin by using p-nitrophenyl N2-acetyl-N1-benzylcarbazate Biochem J (1968) 107, 103-107. PubMed Europe PubMed PMC EPMC I
Sigler,P.B., Blow,D.M., Matthews,B.W. and Henderson,R. Structure of crystalline alpha-chymotrypsin. II. A preliminary report including a hypothesis for the activation mechanism J Mol Biol (1968) 35, 143-164. PubMed Europe PubMed

1967

Matthews,B.W., Sigler,P.B., Henderson,R. and Blow,D.M. Three-dimensional structure of tosyl-alpha-chymotrypsin Nature (1967) 214, 652-656. PubMed Europe PubMed DOI S
Rovery,M. [25] Chromatography of trypsin to remove chymotrypsin, and of chymotrypsin to remove trypsin Methods Enzymol (1967) 11, 231-236. DOI
Smillie,L.B. and Hartley,B.S. The disulphide bridges of bovine chymotrypsinogen B Biochem J (1967) 105, 1125-1133. PubMed Europe PubMed PMC EPMC

1966

Bender,M.L., Begue-Canton,M.L., Blakeley,R.L., Brubacher,L.J., Feder,J., Gunter,C.R., Kezdy,F.J., Killheffer,J.V., Jr., Marshall,T.H., Miller,C.G., Roeske,R.W. and Stoops,J.K. The determination of the concentration of hydrolytic enzyme solutions: alpha-chymotrypsin, trypsin, papain, elastase, subtilisin, and acetylcholinesterase J Am Chem Soc (1966) 88, 5890-5913. PubMed Europe PubMed
Brown,J.R. and Hartley,B.S. Location of disulphide bridges by diagonal paper electrophoresis. The disulphide bridges of bovine chymotrypsinogen A Biochem J (1966) 101, 214-228. PubMed Europe PubMed PMC EPMC
[YEAR:25-11-1966]Feinstein,G. and Feeney,R.E. Interaction of inactive derivatives of chymotrypsin and trypsin with protein inhibitors J Biol Chem (25-11-1966) 241, 5183-5189. PubMed Europe PubMed
Gold,A.M. and Fahrney,D. The kinetic equivalence of reactivated phenylmethanesulfonyl-alpha-chymotrypsin and native alpha-chymotrypsin. A reexamination Biochemistry (1966) 5, 2911-2913. PubMed Europe PubMed I
Rapp,J.R., Niemann,C. and Hein,G.E. The primary specificity of chymotrypsin. Further evidence for "wrong-way" binding Biochemistry (1966) 5, 4100-4105.
Sigler,P.B., Jeffery,B.A., Matthews,B.W. and Blow,D.M. An x-ray diffraction study of inhibited derivatives of alpha-chymotrypsin J Mol Biol (1966) 15, 175-192. PubMed Europe PubMed S

1965

Abrash,H.I. and Niemann,C. Interaction of aromatic compounds with alpha-chymotrypsin. II. Combination of the isomeric indole carboxamides and carboxylate ions with the active site Biochemistry (1965) 4, 99-104. PubMed Europe PubMed
Gold,A.M. Sulfonyl fluorides as inhibitors of esterases. 3. Identification of serine as the site of sulfonylation in phenylmethanesulfonyl alpha-chymotrypsin Biochemistry (1965) 4, 897-901. PubMed Europe PubMed I
Hartley,B.S., Brown,J.R., Kauffman,D.L. and Smillie,L.B. Evolutionary similarities between pancreatic proteolytic enzymes Nature (1965) 207, 1157-1159. PubMed Europe PubMed DOI
Jones,J.B., Niemann,C. and Hein,G.E. The primary specificity of alpha-chymotrypsin. Interaction with acylated derivatives of D-valine methyl ester and D-norvaline methyl ester Biochemistry (1965) 4, 1735-1739.
Jones,J.B., Kunitake,T., Niemann,C. and Hein,G.E. The primary specificity of alpha-chymotrypsin. Acylated amino acid esters with normal alkyl side chains J Am Chem Soc (1965) 87, 1777-1781. PubMed Europe PubMed
Lawson,W.B. and Schramm,H.J. Modification of a methionine residue near the active site of chymotrypsin by p-nitrophenyl bromoacetyl-alpha-aminoisobutyrate Biochemistry (1965) 4, 377-386. PubMed Europe PubMed

1964

Bender,M.L., Kezdy,F.J. and Gunter,C.R. The anatomy of an enzymatic catalysis: alpha-chymotrypsin J Am Chem Soc (1964) 86, 3714-3721.
Bender,M.L., Clement,G.E., Kezdy,F.J. and Heck,H.D. The correlation of the pH (pD) dependence and the stepwise mechanism of alpha-chymotrypsin-catalyzed reactions J Am Chem Soc (1964) 86, 3680-3690.
Bender,M.L., Killheffer,J.V., Jr. and Kezdy,F.J. The active site of chymotrypsin J Am Chem Soc (1964) 86, 5331-5333.
Bender,M.L. and Kezdy,F.J. The current status of the alpha-chymotrypsin mechanism J Am Chem Soc (1964) 86, 3704-3714.
Cheung,H.T., Murthy,T.S. and Blout,E.R. The synthesis of an octapeptide corresponding to a sequence around the "reactive serine" of chymotrypsin J Am Chem Soc (1964) 86, 4200-4202.
Cohen,S.G. and Crossley,J. Kinetics of hydrolysis of diethyl glutarate and beta-substituted diethyl glutarates by alpha-chymotrypsin J Am Chem Soc (1964) 86, 1217-1220.
Cohen,S.G. and Weinstein,S.Y. Hydrolysis of D-(-)-ethyl alpha-benzoyloxypropionate and L-(-)-ethyl alpha-acetoxy-beta-phenylpropionate by alpha-chymotrypsin J Am Chem Soc (1964) 86, 5326-5330.
Cohen,S.G. and Crossley,J. Kinetics of hydrolysis of dicarboxylic esters and their alpha-acetamido derivatives by alpha-chymotrypsin J Am Chem Soc (1964) 86, 4999-5003.
Erlanger,B.F., Cooper,A.G. and Bendich,A.J. On the heterogeneity of three-times-crystallised alpha-chymotrypsin Biochemistry (1964) 3, 1880-1883. PubMed Europe PubMed
Erlanger,B.F. and Edel,F. The utilization of a specific chromogenic inactivator in an "all or none" assay for chymotrypsin Biochemistry (1964) 3, 346-349. PubMed Europe PubMed
Hartley,B.S. Amino-acid sequence of bovine chymotrypsinogen-A Nature (1964) 201, 1284-1287. PubMed Europe PubMed DOI
Kezdy,F.J. and Bender,M.L. The acylation of alpha-chymotrypsin by N-acetyl-L-tryptophan J Am Chem Soc (1964) 86, 938-940.
Kezdy,F.J. and Bender,M.L. The observation of acyl-enzyme intermediates in the alpha-chymotrypsin-catalyzed hydrolysis of specific ester substrates at low pH J Am Chem Soc (1964) 86, 937-938.
Kezdy,F.J., Clement,G.E. and Bender,M.L. The observation of acyl-enzyme intermediates in the alpha-chymotrypsin-catalyzed reactions of N-acetyl-L-tryptophan derivatives at low pH J Am Chem Soc (1964) 86, 3690-3696.
Lagunoff,D. and Benditt,E.P. Benzosalicylanilide ester substrates of proteolytic enzymes. Kinetic and histochemical studies. I. Chymotrypsin Biochemistry (1964) 3, 1427-1431. PubMed Europe PubMed
Meloun,B. and Pospisilova,D. The histidine residue involved in the proteolytic activity of alpha-chymotrypsin Biochim Biophys Acta (1964) 92, 152-153. PubMed Europe PubMed
Ong,E.B., Shaw,E. and Schoellmann,G. An active center histidine peptide of alpha-chymotrypsin J Am Chem Soc (1964) 86, 1271-1272.
Walsh,K.A. and Neurath,H. Trypsinogen and chymotrypsinogen as homologous proteins Proc Natl Acad Sci U S A (1964) 52, 884-889. PubMed Europe PubMed PMC EPMC
Walsh,K.A., Kauffman,D.L., Kumar,K.S. and Neurath,H. ON THE STRUCTURE AND FUNCTION OF BOVINE TRYPSINOGEN AND TRYPSIN Proc Natl Acad Sci U S A (1964) 51, 301-308. PubMed Europe PubMed PMC EPMC
Wolf,J.P., III, Wallace,R.A., Peterson,R.L. and Niemann,C. The alpha-chymotrypsin-catalyzed hydrolysis of a series of acylated glycine methyl esters. II. Behavior at low and high substrate concentrations Biochemistry (1964) 3, 940-944. PubMed Europe PubMed
Zerner,B. and Bender,M.L. The kinetic consequences of the acyl-enzyme mechanism for the reactions of specific substrates with chymotrypsin J Am Chem Soc (1964) 86, 3669-3674.
Zerner,B., Bond,R.P.M. and Bender,M.L. Kinetic evidence for the formation of acyl-enzyme intermediates in the alpha-chymotrypsin-catalyzed hydrolyses of specific substrates J Am Chem Soc (1964) 86, 3674-3679.

1963

Abrash,H.I. and Niemann,C. Steric hindrance in alpha-chymotrypsin-catalyzed reactions Biochemistry (1963) 2, 947-953. PubMed Europe PubMed
Bender,M.L. and Clement,G.E. Kinetic evidence for an acyl-enzyme intermediate in the alpha-chymotrypsin-catalyzed hydrolysis of N-acetyl-L-tryptophan ethyl ester Biochem Biophys Res Commun (1963) 12, 339-344. PubMed Europe PubMed
Fahrney,D.E. and Gold,A.M. Sulfonyl fluorides as inhibitors of esterases. I. Rates of reaction with acetylcholinesterase, alpha-chymotrypsin, and trypsin J Am Chem Soc (1963) 85, 997-1000. DOI I
[YEAR:18-1-1963]Gold,A.M. and Fahrney,D.E. The mechanism of reactivation of phenylmethanesulfonyl alpha-chymotrypsin Biochem Biophys Res Commun (18-1-1963) 10, 55-59. PubMed Europe PubMed I
Keil,B., Prusik,Z. and Sorm,F. Disulphide bridges and a suggested structure of chymotrypsinogen Biochim Biophys Acta (1963) 78, 559-561. PubMed Europe PubMed
Schoellmann,G. and Shaw,E. Direct evidence for the presence of histidine in the active center of chymotrypsin Biochemistry (1963) 2, 252-255. PubMed Europe PubMed I
Wallace,R.A., Kurtz,A.N. and Niemann,C. Interaction of aromatic compounds with alpha-chymotrypsin Biochemistry (1963) 2, 824-836. PubMed Europe PubMed
Zerner,B. and Bender,M.L. Acyl-enzyme intermediates in the alpha-chymotrypsin-catalyzed hydrolysis of "specific" substrates. The relative rates of hydrolysis of ethyl, methyl and p-nitrophenyl esters of N-acetyl-L-tryptophan J Am Chem Soc (1963) 85, 356-358. DOI

1962

Balls,A.K. Early experiments in the quest of an active center in chymotrypsin J Gen Physiol (1962) 45, 47-56. PubMed Europe PubMed
Bender,M.L. The mechanism of alpha-chymotrypsin-catalyzed hydrolyses J Am Chem Soc (1962) 84, 2582-2590.
Bender,M.L., Schonbaum,G.R. and Zerner,B. Spectrophotometric investigations of the mechanism of alpha-chymotrypsin-catalyzed hydrolyses. Detection of the acyl-enzyme intermediate J Am Chem Soc (1962) 84, 2540-2550.
Bender,M.L., Schonbaum,G.R. and Zerner,B. The pH dependence of some alpha-chymotrypsin-catalyzed hydrolyses J Am Chem Soc (1962) 84, 2562-2570.
Hartley,B.S. On the structure of chymotrypsin Brookhaven Symp Biol (1962) 15, 85-100. PubMed Europe PubMed
Hein,G.E. and Niemann,C. Steric course and specificity of alpha-chymotrypsin-catalyzed reactions. II J Am Chem Soc (1962) 84, 4495-4503.
Kassell,B. and Laskowski,M., Sr. A comparison of the terminal amino acids of chymotrypsinogen B and alpha-chymotrypsinogen J Biol Chem (1962) 237, 413-417. PubMed Europe PubMed
Kezdy,F.J. and Bender,M.L. The kinetics of the alpha-chymotrypsin-catalyzed hydrolysis of p-nitrophenyl acetate Biochemistry (1962) 1, 1097-1106. PubMed Europe PubMed
Waite,H.R. and Niemann,C. The alpha-chymotrypsin-catalyzed hydrolysis of a series of acylated-L-valine esters Biochemistry (1962) 1, 250-253. PubMed Europe PubMed

1961

Bruice,T.C. The mechanisms for chymotrypsin Proc Natl Acad Sci U S A (1961) 47, 1924-1928. PubMed Europe PubMed PMC EPMC
Cohen,S.G., Sprinzak,Y. and Khedouri,E. Requirements for stereospecificity in hydrolysis by alpha-chymotrypsin. III. The acylamido group J Am Chem Soc (1961) 83, 4225-4228.
Kurtz,A.N. and Niemann,C. The interaction of alpha-chymotrypsin with 1-acyl-2-(alpha-aminoacyl)hydrazines Biochim Biophys Acta (1961) 53, 324-331. PubMed Europe PubMed I
Schonbaum,G.R., Zerner,B. and Bender,M.L. The spectrophotometric determination of the operational normality of an alpha-chymotrypsin solution J Biol Chem (1961) 236, 2930-2935. PubMed Europe PubMed A

1960

Bender,M.L. and Glasson,W.A. The kinetics of the alpha-chymotrypsin-catalyzed hydrolysis and methanolysis of acetyl-L-phenylalanine methyl ester. Evidence for the specific binding of water on the enzyme surface J Am Chem Soc (1960) 82, 3336-3342.
Yamashita,T. Action of chymotrypsin on synthetic substrates. V. Action of alpha-chymotrypsin on glycyl-l-aminoacyl-l-tyrosinamides J Biochem (1960) 48, 846-852.

1959

Hartley,B.S. The chemical structure of chymotrypsin J Cell Comp Physiol (1959) 54, 203-205. PubMed Europe PubMed
Hummel,B.C. A modified spectrophotometric determination of chymotrypsin, trypsin, and thrombin Can J Biochem Physiol (1959) 37, 1393-1399. PubMed Europe PubMed A
Martin,C.J., Golubow,J. and Axelrod,A.E. A rapid and sensitive spectrophotometric method for the assay of chymotrypsin J Biol Chem (1959) 234, 294-298. PubMed Europe PubMed
Yamashita,T. and Izumiya,N. Action of chymotrypsin on synthetic substrates. III. Action of alpha-chymotrypsin on aminoacyl-L-tyrosine amides and ethyl esters J Biochem (1959) 46, 991-1000.

1957

Wilcox,P.E., Kraut,J., Wade,R.D. and Neurath,H. The molecular weight of alpha-chymotrypsinogen Biochim Biophys Acta (1957) 24, 72-78. PubMed Europe PubMed

1956

Bluhm,M.M. and Kendrew,J.C. The crystal forms and molecular weight of alpha-chymotrypsinogen. An X-ray study Biochim Biophys Acta Gen Subj (1956) 20, 562-563. PubMed Europe PubMed
Cunningham,L.W. and Brown,C.S. The influence of pH on the kinetic constants of alpha-chymotrypsin-catalyzed esterolysis J Biol Chem (1956) 221, 287-299. PubMed Europe PubMed
Davis,N.C. Action of proteolytic enzymes on some peptides and derivatives containing histidine J Biol Chem (1956) 223, 935-947. PubMed Europe PubMed P

1955

Bettelheim,F.R. and Neurath,H. The rapid activation of chymotrypsinogen J Biol Chem (1955) 212, 241-253. PubMed Europe PubMed
Foster,R.J. and Niemann,C. Re-evaluation of the kinetic constants of previously investigated specific substrates of alpha-chymotrypsin J Am Chem Soc (1955) 77, 1886-1892.
Laskowski,M. Chymotrypsinogens and chymotrypsins Methods Enzymol (1955) 2, 8-26. DOI
Sakota,N., Yu,T., Okada,Y. and Yamada,H. A new crystalline form of chymotrypsin J Biochem (1955) 42, 609-611.
Schwert,G.W. and Takenaka,Y. A spectrophotometric determination of trypsin and chymotrypsin Biochim Biophys Acta (1955) 16, 570-575. PubMed Europe PubMed A

1954

Gladner,J.A. and Neurath,H. Carboxyl terminal groups of proteolytic enzymes. II. Chymotrypsins J Biol Chem (1954) 206, 911-924. PubMed Europe PubMed

1952

Gladner,J.A. and Neurath,H. C-terminal groups in chymotrypsinogen and DFP-alpha-chymotrypsin in relation to the activation process Biochim Biophys Acta (1952) 9, 335-336. PubMed Europe PubMed
Hartley,B.S. and Kilby,B.A. The inhibition of chymotrypsin by diethyl p-nitrophenyl phosphate Biochem J (1952) 50, 672-678. PubMed Europe PubMed I
Loewus,M.W. and Briggs,D.R. The number of catalytically active sites present on the chymotrypsin molecule J Biol Chem (1952) 199, 857-864. PubMed Europe PubMed

1951

Huang,H.T. and Niemann,C. The evaluation of the enzyme-inhibitor dissociation constant of alpha-chymotrypsin and acetyl-D-tryptophan methyl ester J Am Chem Soc (1951) 73, 3228-3231.
Sanger,F. and Tuppy,H. Amino-acid sequence in the phenylalanyl chain of insulin. 2. The investigation of peptides from enzymic hydrolysates Biochem J (1951) 49, 481-490. PubMed Europe PubMed PMC EPMC P
Seligman,A.M. and Wolf,G. Synthesis of peptides and esters of phenylalanine as chromogenic substrates for chymotrypsin J Am Chem Soc (1951) 73, 2086-2089. A

1950

Iselin,B.M., Huang,H.T., MacAllister,R.V. and Niemann,C. A new series of substrates for the evaluation of chymotrypsin activity J Am Chem Soc (1950) 72, 1729-1731. A
Neurath,H. and Schwert,G.W. The mode of action of the crystalline pancreatic proteolytic enzymes Chem Rev (1950) 46, 69-153. PubMed Europe PubMed V

1949

Kaufman,S. and Neurath,H. Structural requirements of specific inhibitors for alpha-chymotrypsin J Biol Chem (1949) 181, 623-633. PubMed Europe PubMed

1948

Kaufman,S., Schwert,G.W. and Neurath,H. The specific peptidase and esterase activities of chymotrypsin Arch Biochem (1948) 17, 203-205. PubMed Europe PubMed
[YEAR:20-11-1948]Kunitz,M. Crystallization of salt-free chymotrypsinogen and chymotrypsin from solution in dilute ethyl alcohol J Gen Physiol (20-11-1948) 32, 265-269. PubMed Europe PubMed DOI

1945

Sizer,I.W. Essential groups of crystalline chymotrypsin J Biol Chem (1945) 160, 547-554. PubMed Europe PubMed

1941

Butler,J.A.V. On the formation of chymotrypsin from chymotrypsinogen J Am Chem Soc (1941) 63, 2968-2970.

1938

[YEAR:20-11-1938]Kunitz,M. Formation of new crystalline enzymes from chymotrypsin: isolation of beta and gamma chymotrypsin J Gen Physiol (20-11-1938) 22, 207-237. PubMed Europe PubMed DOI

1937

Bergmann,M. and Fruton,J.S. On proteolytic enzymes. XIII. Synthetic substrates for chymotrypsin J Biol Chem (1937) 118, 405-415.

1935

[YEAR:20-3-1935]Kunitz,M. A method for determining the rennet activity of chymo-trypsin J Gen Physiol (20-3-1935) 18, 459-466. PubMed Europe PubMed DOI
Kunitz,M. and Northrop,J.H. Crystalline chymo-trypsin and chymo-trypsinogen. I. Isolation, crystallization, and general properties of a new proteolytic enzyme and its precursor J Gen Physiol (1935) 18, 433-458. PubMed Europe PubMed DOI

1933

Kunitz,M. and Northrop,J.H. Isolation of a crystalline protein from pancreas and its conversion into a new crystalline proteolytic enzyme by trypsin Science (1933) 78, 558-559. PubMed Europe PubMed DOI