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Literature for peptidase M04.018: stearolysin

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

(Topics flags: V Review. To select only the references relevant to a single topic, click the link above. See explanation.)

    2026
  1. Ramchunder,K., Anderson,M.H. and Senzani,S.<br>Bacterial zinc metalloproteases as facilitators of pathogenic success: a review<br>Arch Microbiol (2026) 208, 97-97. PubMed  Europe PubMed DOI
    2. 2012
  2. Mansfeld,J.<br>Stearolysin<br>[ISSN:978-0-12-407744-7] (2012) 3, 554-560. DOI
    3. 2011
  3. Eijsink,V., Mathews,B. and Vriend,G.<br>The role of calcium ions in the stability and instability of a thermolysin-like protease<br>Protein Sci (2011) 20, 1346-1355. PubMed  Europe PubMed DOI
    4. 2010
  4. Durrschmidt,P., Mansfeld,J. and Ulbrich-Hofmann,R.<br>Refolding of the non-specific neutral protease from Bacillus stearothermophilus proceeds via an autoproteolytically sensitive intermediate<br>Biophys Chem (2010) 147, 66-73. PubMed  Europe PubMed DOI
    5. 2007
  5. Mansfeld,J. and Ulbrich-Hofmann,R.<br>The stability of engineered thermostable neutral proteases from Bacillus stearothermophilus in organic solvents and detergents<br>Biotechnol Bioeng (2007) 97, 672-679. PubMed  Europe PubMed DOI
    6. 2005
  6. Durrschmidt,P., Mansfeld,J. and Ulbrich-Hofmann,R.<br>An engineered disulfide bridge mimics the effect of calcium to protect neutral protease against local unfolding<br>FEBS J (2005) 272, 1523-1534. PubMed  Europe PubMed DOI
  7. Mansfeld,J., Petermann,E., Durrschmidt,P. and Ulbrich-Hofmann,R.<br>The propeptide is not required to produce catalytically active neutral protease from Bacillus stearothermophilus<br>Protein Expr Purif (2005) 39, 219-228. PubMed  Europe PubMed DOI
    8. 2002
  8. [YEAR:3-5-2002]de Kreij,A., Van Den Burg,B., Venema,G., Vriend,G., Eijsink,V.G. and Nielsen,J.E.<br>The effects of modifying the surface charge on the catalytic activity of a thermolysin-like protease<br>J Biol Chem (3-5-2002) 277, 15432-15438. PubMed  Europe PubMed DOI
  9. Kuhn,D., Durrschmidt,P., Mansfeld,J. and Ulbrich-Hofmann,R.<br>Boilysin and thermolysin in dipeptide synthesis: a comparative study<br>Biotechnol Appl Biochem (2002) 36, 71-76. PubMed  Europe PubMed DOI
    10. 2001
  10. Durrschmidt,P., Mansfeld,J. and Ulbrich-Hofmann,R.<br>Differentiation between conformational and autoproteolytic stability of the neutral protease from Bacillus stearothermophilus containing an engineered disulfide bond<br>Eur J Biochem (2001) 268, 3612-3618. PubMed  Europe PubMed DOI
    11. 2000
  11. [YEAR:6-10-2000]de Kreij,A., Venema,G. and Van Den Burg,B.<br>Substrate specificity in the highly heterogeneous M4 peptidase family is determined by a small subset of amino acids<br>J Biol Chem (6-10-2000) 275, 31115-31120. PubMed  Europe PubMed DOI
  12. Mansfeld,J. and Ulbrich-Hofmann,R.<br>Site-specific and random immobilization of thermolysin-like proteases reflected in the thermal inactivation kinetics<br>Biotechnol Appl Biochem (2000) 32, 189-195. PubMed  Europe PubMed DOI
  13. Sookkheo,B., Sinchaikul,S., Phutrakul,S. and Chen,S.T.<br>Purification and characterization of the highly thermostable proteases from Bacillus stearothermophilus TLS33<br>Protein Expr Purif (2000) 20, 142-151. PubMed  Europe PubMed DOI
    14. 1999
  14. [YEAR:29-6-1999]Mansfeld,J., Vriend,G., Van Den Burg,B., Eijsink,V.G. and Ulbrich-Hofmann,R.<br>Probing the unfolding region in a thermolysin-like protease by site-specific immobilization<br>Biochemistry (29-6-1999) 38, 8240-8245. PubMed  Europe PubMed DOI
  15. Van Den Burg,B., de Kreij,A., Winkel,C. and Venema,G.<br>Hydrolysis of industrial substrates by an extremely stable thermolysin-like protease variant obtained by protein engineering<br>Biotechnol Lett (1999) 21, 537-542. DOI
    16. 1998
  16. [YEAR:14-4-1998]Veltman,O.R., Eijsink,V.G., Vriend,G., de Kreij,A., Venema,G. and Van Den Burg,B.<br>Probing catalytic hinge bending motions in thermolysin-like proteases by glycine - > alanine mutations<br>Biochemistry (14-4-1998) 37, 5305-5311. PubMed  Europe PubMed DOI
  17. [YEAR:14-4-1998]Veltman,O.R., Vriend,G., Berendsen,H.J., Van Den Burg,B., Venema,G. and Eijsink,V.G.<br>A single calcium binding site is crucial for the calcium-dependent thermal stability of thermolysin-like proteases<br>Biochemistry (14-4-1998) 37, 5312-5319. PubMed  Europe PubMed DOI
    18. 1997
  18. [YEAR:25-4-1997]Mansfeld,J., Vriend,G., Dijkstra,B.W., Veltman,O.R., Van Den Burg,B., Venema,G., Ulbrich-Hofmann,R. and Eijsink,V.G.<br>Extreme stabilization of a thermolysin-like protease by an engineered disulfide bond<br>J Biol Chem (25-4-1997) 272, 11152-11156. PubMed  Europe PubMed DOI
  19. Nakamura,S., Tanaka,T., Yada,R.Y. and Nakai,S.<br>Improving the thermostability of Bacillus stearothermophilus neutral protease by introducing proline into the active site helix<br>Protein Eng (1997) 10, 1263-1269. PubMed  Europe PubMed
  20. Veltman,O.R., Vriend,G., Hardy,F., Mansfeld,J., Van Den Burg,B., Venema,G. and Eijsink,V.G.<br>Mutational analysis of a surface area that is critical for the thermal stability of thermolysin-like proteases<br>Eur J Biochem (1997) 248, 433-440. PubMed  Europe PubMed
  21. Veltman,O.R., Vriend,G., Van Den Burg,B., Hardy,F., Venema,G. and Eijsink,V.G.<br>Engineering thermolysin-like proteases whose stability is largely independent of calcium<br>FEBS Lett (1997) 405, 241-244. PubMed  Europe PubMed DOI
    22. 1996
  22. Veltman,O.R., Vriend,G., Middelhoven,P.J., Van Den Burg,B., Venema,G. and Eijsink,V.G.<br>Analysis of structural determinants of the stability of thermolysin-like proteases by molecular modelling and site-directed mutagenesis<br>Protein Eng (1996) 9, 1181-1189. PubMed  Europe PubMed
    23. 1995
  23. Eijsink,V.G., Veltman,O.R., Aukema,W., Vriend,G. and Venema,G.<br>Structural determinants of the stability of thermolysin-like proteinases<br>Nat Struct Biol (1995) 2, 374-379. PubMed  Europe PubMed DOI
  24. Sidorenkov,I.N., Nosovskaia,E.A. and Kostrov,S.V.<br>[Cloning and expression of the Bacillus stearothermophilus neutral proteinase gene in Bacillus subtilis cells]<br>Mol Biol (Mosk) (1995) 29, 507-511. PubMed  Europe PubMed
    25. 1994
  25. O'Donohue,M.J., Roques,B.P. and Beaumont,A.<br>Cloning and expression in Bacillus subtilis of the npr gene from Bacillus thermoproteolyticus Rokko coding for the thermostable metalloprotease thermolysin<br>Biochem J (1994) 300, 599-603. PubMed  Europe PubMed  PMC  EPMC
    26. 1993
  26. Hardy,F., Vriend,G., Veltman,O.R., van der Vinne,B., Venema,G. and Eijsink,V.G.<br>Stabilization of Bacillus stearothermophilus neutral protease by introduction of prolines<br>FEBS Lett (1993) 317, 89-92. PubMed  Europe PubMed DOI
  27. Vriend,G. and Eijsink,V.<br>Prediction and analysis of structure, stability and unfolding of thermolysin-like proteases<br>J Comput Aided Mol Des (1993) 7, 367-396. PubMed  Europe PubMed  V
    28. 1992
  28. Eijsink,V.G., Vriend,G., van der Vinne,B., Hazes,B., Van Den Burg,B. and Venema,G.<br>Effects of changing the interaction between subdomains on the thermostability of Bacillus neutral proteases<br>Proteins (1992) 14, 224-236. PubMed  Europe PubMed DOI
  29. Kubo,M., Mitsuda,Y., Takagi,M. and Imanaka,T.<br>Alteration of specific activity and stability of thermostable neutral protease by site-directed mutagenesis<br>Appl Environ Microbiol (1992) 58, 3779-3783. PubMed  Europe PubMed  PMC  EPMC
    30. 1991
  30. Eijsink,V.G., Van Den Burg,B. and Venema,G.<br>High performance affinity chromatography of Bacillus neutral proteases<br>Biotechnol Appl Biochem (1991) 14, 275-283. PubMed  Europe PubMed
    31. 1990
  31. Nishiya,Y. and Imanaka,T.<br>Cloning and nucleotide sequences of the Bacillus stearothermophilus neutral protease gene and its transcriptional activator gene<br>J Bacteriol (1990) 172, 4861-4869. PubMed  Europe PubMed  PMC  EPMC
    32. 1988
  32. Kubo,M. and Imanaka,T.<br>Cloning and nucleotide sequence of the highly thermostable neutral protease gene from Bacillus stearothermophilus<br>J Gen Microbiol (1988) 134, 1883-1892. PubMed  Europe PubMed
    33. 1985
  33. Takagi,M., Imanaka,T. and Aiba,S.<br>Nucleotide sequence and promoter region for the neutral protease gene from Bacillus stearothermophilus<br>J Bacteriol (1985) 163, 824-831. PubMed  Europe PubMed  PMC  EPMC
    34. 1983
  34. Fujii,M., Takagi,M., Imanaka,T. and Aiba,S.<br>Molecular cloning of a thermostable neutral protease gene from Bacillus stearothermophilus in a vector plasmid and its expression in Bacillus stearothermophilus and Bacillus subtilis<br>J Bacteriol (1983) 154, 831-837. PubMed  Europe PubMed  PMC  EPMC
    35. 1980
  35. Sidler,W. and Zuber,H.<br>Isolation procedures for thermostable neutral proteinases produced by Bacillus stearothermophilus<br>Eur J Appl Microbiol Biotechnol (1980) 10, 197-209.
    36. 1972
  36. Sidler,W. and Zuber,H.<br>Neutral proteases with different thermostabilities from a facultative strain of Bacillus stearothermophilus grown at 40 degrees and at 50 degrees<br>FEBS Lett (1972) 25, 292-294. PubMed  Europe PubMed

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