Merops Switch to
Inhibitors

Peptidase

Family Clan SEARCHES BLAST
MEROPS SUBMISSIONS

Other
information

Literature for peptidase M04.018: stearolysin

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

(Topics flags: V Review. To select only the references relevant to a single topic, click the link above. See explanation.)

    2012
  1. Mansfeld,J.
    Stearolysin
    [ISSN:978-0-12-407744-7]3, 554-560. DOI
    2. 2011
  2. Eijsink,V., Mathews,B. and Vriend,G.
    The role of calcium ions in the stability and instability of a thermolysin-like protease
    Protein Sci20, 1346-1355. PubMed  Europe PubMed DOI
    3. 2010
  3. Durrschmidt,P., Mansfeld,J. and Ulbrich-Hofmann,R.
    Refolding of the non-specific neutral protease from Bacillus stearothermophilus proceeds via an autoproteolytically sensitive intermediate
    Biophys Chem147, 66-73. PubMed  Europe PubMed DOI
    4. 2007
  4. Mansfeld,J. and Ulbrich-Hofmann,R.
    The stability of engineered thermostable neutral proteases from Bacillus stearothermophilus in organic solvents and detergents
    Biotechnol Bioeng97, 672-679. PubMed  Europe PubMed DOI
    5. 2005
  5. Durrschmidt,P., Mansfeld,J. and Ulbrich-Hofmann,R.
    An engineered disulfide bridge mimics the effect of calcium to protect neutral protease against local unfolding
    FEBS J272, 1523-1534. PubMed  Europe PubMed DOI
  6. Mansfeld,J., Petermann,E., Durrschmidt,P. and Ulbrich-Hofmann,R.
    The propeptide is not required to produce catalytically active neutral protease from Bacillus stearothermophilus
    Protein Expr Purif39, 219-228. PubMed  Europe PubMed DOI
    7. 2002
  7. [YEAR:3-5-2002]de Kreij,A., Van Den Burg,B., Venema,G., Vriend,G., Eijsink,V.G. and Nielsen,J.E.
    The effects of modifying the surface charge on the catalytic activity of a thermolysin-like protease
    J Biol Chem277, 15432-15438. PubMed  Europe PubMed DOI
  8. Kuhn,D., Durrschmidt,P., Mansfeld,J. and Ulbrich-Hofmann,R.
    Boilysin and thermolysin in dipeptide synthesis: a comparative study
    Biotechnol Appl Biochem36, 71-76. PubMed  Europe PubMed DOI
    9. 2001
  9. Durrschmidt,P., Mansfeld,J. and Ulbrich-Hofmann,R.
    Differentiation between conformational and autoproteolytic stability of the neutral protease from Bacillus stearothermophilus containing an engineered disulfide bond
    Eur J Biochem268, 3612-3618. PubMed  Europe PubMed DOI
    10. 2000
  10. [YEAR:6-10-2000]de Kreij,A., Venema,G. and Van Den Burg,B.
    Substrate specificity in the highly heterogeneous M4 peptidase family is determined by a small subset of amino acids
    J Biol Chem275, 31115-31120. PubMed  Europe PubMed DOI
  11. Mansfeld,J. and Ulbrich-Hofmann,R.
    Site-specific and random immobilization of thermolysin-like proteases reflected in the thermal inactivation kinetics
    Biotechnol Appl Biochem32, 189-195. PubMed  Europe PubMed DOI
  12. Sookkheo,B., Sinchaikul,S., Phutrakul,S. and Chen,S.T.
    Purification and characterization of the highly thermostable proteases from Bacillus stearothermophilus TLS33
    Protein Expr Purif20, 142-151. PubMed  Europe PubMed DOI
    13. 1999
  13. [YEAR:29-6-1999]Mansfeld,J., Vriend,G., Van Den Burg,B., Eijsink,V.G. and Ulbrich-Hofmann,R.
    Probing the unfolding region in a thermolysin-like protease by site-specific immobilization
    Biochemistry38, 8240-8245. PubMed  Europe PubMed DOI
  14. Van Den Burg,B., de Kreij,A., Winkel,C. and Venema,G.
    Hydrolysis of industrial substrates by an extremely stable thermolysin-like protease variant obtained by protein engineering
    Biotechnol Lett21, 537-542. DOI
    15. 1998
  15. [YEAR:14-4-1998]Veltman,O.R., Eijsink,V.G., Vriend,G., de Kreij,A., Venema,G. and Van Den Burg,B.
    Probing catalytic hinge bending motions in thermolysin-like proteases by glycine - > alanine mutations
    Biochemistry37, 5305-5311. PubMed  Europe PubMed DOI
  16. [YEAR:14-4-1998]Veltman,O.R., Vriend,G., Berendsen,H.J., Van Den Burg,B., Venema,G. and Eijsink,V.G.
    A single calcium binding site is crucial for the calcium-dependent thermal stability of thermolysin-like proteases
    Biochemistry37, 5312-5319. PubMed  Europe PubMed DOI
    17. 1997
  17. [YEAR:25-4-1997]Mansfeld,J., Vriend,G., Dijkstra,B.W., Veltman,O.R., Van Den Burg,B., Venema,G., Ulbrich-Hofmann,R. and Eijsink,V.G.
    Extreme stabilization of a thermolysin-like protease by an engineered disulfide bond
    J Biol Chem272, 11152-11156. PubMed  Europe PubMed DOI
  18. Nakamura,S., Tanaka,T., Yada,R.Y. and Nakai,S.
    Improving the thermostability of Bacillus stearothermophilus neutral protease by introducing proline into the active site helix
    Protein Eng10, 1263-1269. PubMed  Europe PubMed
  19. Veltman,O.R., Vriend,G., Van Den Burg,B., Hardy,F., Venema,G. and Eijsink,V.G.
    Engineering thermolysin-like proteases whose stability is largely independent of calcium
    FEBS Lett405, 241-244. PubMed  Europe PubMed DOI
  20. Veltman,O.R., Vriend,G., Hardy,F., Mansfeld,J., Van Den Burg,B., Venema,G. and Eijsink,V.G.
    Mutational analysis of a surface area that is critical for the thermal stability of thermolysin-like proteases
    Eur J Biochem248, 433-440. PubMed  Europe PubMed
    21. 1996
  21. Veltman,O.R., Vriend,G., Middelhoven,P.J., Van Den Burg,B., Venema,G. and Eijsink,V.G.
    Analysis of structural determinants of the stability of thermolysin-like proteases by molecular modelling and site-directed mutagenesis
    Protein Eng9, 1181-1189. PubMed  Europe PubMed
    22. 1995
  22. Eijsink,V.G., Veltman,O.R., Aukema,W., Vriend,G. and Venema,G.
    Structural determinants of the stability of thermolysin-like proteinases
    Nat Struct Biol2, 374-379. PubMed  Europe PubMed DOI
  23. Sidorenkov,I.N., Nosovskaia,E.A. and Kostrov,S.V.
    [Cloning and expression of the Bacillus stearothermophilus neutral proteinase gene in Bacillus subtilis cells]
    Mol Biol (Mosk)29, 507-511. PubMed  Europe PubMed
    24. 1994
  24. O'Donohue,M.J., Roques,B.P. and Beaumont,A.
    Cloning and expression in Bacillus subtilis of the npr gene from Bacillus thermoproteolyticus Rokko coding for the thermostable metalloprotease thermolysin
    Biochem J300, 599-603. PubMed  Europe PubMed
    25. 1993
  25. Hardy,F., Vriend,G., Veltman,O.R., van der Vinne,B., Venema,G. and Eijsink,V.G.
    Stabilization of Bacillus stearothermophilus neutral protease by introduction of prolines
    FEBS Lett317, 89-92. PubMed  Europe PubMed DOI
  26. Vriend,G. and Eijsink,V.
    Prediction and analysis of structure, stability and unfolding of thermolysin-like proteases
    J Comput Aided Mol Des7, 367-396. PubMed  Europe PubMed  V
    27. 1992
  27. Eijsink,V.G., Vriend,G., van der Vinne,B., Hazes,B., Van Den Burg,B. and Venema,G.
    Effects of changing the interaction between subdomains on the thermostability of Bacillus neutral proteases
    Proteins14, 224-236. PubMed  Europe PubMed DOI
  28. Kubo,M., Mitsuda,Y., Takagi,M. and Imanaka,T.
    Alteration of specific activity and stability of thermostable neutral protease by site-directed mutagenesis
    Appl Environ Microbiol58, 3779-3783. PubMed  Europe PubMed
    29. 1991
  29. Eijsink,V.G., Van Den Burg,B. and Venema,G.
    High performance affinity chromatography of Bacillus neutral proteases
    Biotechnol Appl Biochem14, 275-283. PubMed  Europe PubMed
    30. 1990
  30. Nishiya,Y. and Imanaka,T.
    Cloning and nucleotide sequences of the Bacillus stearothermophilus neutral protease gene and its transcriptional activator gene
    J Bacteriol172, 4861-4869. PubMed  Europe PubMed
    31. 1988
  31. Kubo,M. and Imanaka,T.
    Cloning and nucleotide sequence of the highly thermostable neutral protease gene from Bacillus stearothermophilus
    J Gen Microbiol134, 1883-1892. PubMed  Europe PubMed
    32. 1985
  32. Takagi,M., Imanaka,T. and Aiba,S.
    Nucleotide sequence and promoter region for the neutral protease gene from Bacillus stearothermophilus
    J Bacteriol163, 824-831. PubMed  Europe PubMed
    33. 1983
  33. Fujii,M., Takagi,M., Imanaka,T. and Aiba,S.
    Molecular cloning of a thermostable neutral protease gene from Bacillus stearothermophilus in a vector plasmid and its expression in Bacillus stearothermophilus and Bacillus subtilis
    J Bacteriol154, 831-837. PubMed  Europe PubMed
    34. 1980
  34. Sidler,W. and Zuber,H.
    Isolation procedures for thermostable neutral proteinases produced by Bacillus stearothermophilus
    Eur J Appl Microbiol Biotechnol10, 197-209.
    35. 1972
  35. Sidler,W. and Zuber,H.
    Neutral proteases with different thermostabilities from a facultative strain of Bacillus stearothermophilus grown at 40 degrees and at 50 degrees
    FEBS Lett25, 292-294. PubMed  Europe PubMed

feedback
Page created 8-September-2023

EMBL-EBI logo

© 2025 EBI
Terms of use