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Literature for peptidase M04.018: stearolysin

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Inhibitors

(Topics flags: V Review. To select only the references relevant to a single topic, click the link above. See explanation.)

    2026
  1. Ramchunder,K., Anderson,M.H. and Senzani,S.
    Bacterial zinc metalloproteases as facilitators of pathogenic success: a review
    Arch Microbiol (2026) 208, 97-97. PubMed  Europe PubMed DOI
    2. 2012
  2. Mansfeld,J.
    Stearolysin
    [ISSN:978-0-12-407744-7] (2012) 3, 554-560. DOI
    3. 2011
  3. Eijsink,V., Mathews,B. and Vriend,G.
    The role of calcium ions in the stability and instability of a thermolysin-like protease
    Protein Sci (2011) 20, 1346-1355. PubMed  Europe PubMed DOI
    4. 2010
  4. Durrschmidt,P., Mansfeld,J. and Ulbrich-Hofmann,R.
    Refolding of the non-specific neutral protease from Bacillus stearothermophilus proceeds via an autoproteolytically sensitive intermediate
    Biophys Chem (2010) 147, 66-73. PubMed  Europe PubMed DOI
    5. 2007
  5. Mansfeld,J. and Ulbrich-Hofmann,R.
    The stability of engineered thermostable neutral proteases from Bacillus stearothermophilus in organic solvents and detergents
    Biotechnol Bioeng (2007) 97, 672-679. PubMed  Europe PubMed DOI
    6. 2005
  6. Durrschmidt,P., Mansfeld,J. and Ulbrich-Hofmann,R.
    An engineered disulfide bridge mimics the effect of calcium to protect neutral protease against local unfolding
    FEBS J (2005) 272, 1523-1534. PubMed  Europe PubMed DOI
  7. Mansfeld,J., Petermann,E., Durrschmidt,P. and Ulbrich-Hofmann,R.
    The propeptide is not required to produce catalytically active neutral protease from Bacillus stearothermophilus
    Protein Expr Purif (2005) 39, 219-228. PubMed  Europe PubMed DOI
    8. 2002
  8. [YEAR:3-5-2002]de Kreij,A., Van Den Burg,B., Venema,G., Vriend,G., Eijsink,V.G. and Nielsen,J.E.
    The effects of modifying the surface charge on the catalytic activity of a thermolysin-like protease
    J Biol Chem (3-5-2002) 277, 15432-15438. PubMed  Europe PubMed DOI
  9. Kuhn,D., Durrschmidt,P., Mansfeld,J. and Ulbrich-Hofmann,R.
    Boilysin and thermolysin in dipeptide synthesis: a comparative study
    Biotechnol Appl Biochem (2002) 36, 71-76. PubMed  Europe PubMed DOI
    10. 2001
  10. Durrschmidt,P., Mansfeld,J. and Ulbrich-Hofmann,R.
    Differentiation between conformational and autoproteolytic stability of the neutral protease from Bacillus stearothermophilus containing an engineered disulfide bond
    Eur J Biochem (2001) 268, 3612-3618. PubMed  Europe PubMed DOI
    11. 2000
  11. [YEAR:6-10-2000]de Kreij,A., Venema,G. and Van Den Burg,B.
    Substrate specificity in the highly heterogeneous M4 peptidase family is determined by a small subset of amino acids
    J Biol Chem (6-10-2000) 275, 31115-31120. PubMed  Europe PubMed DOI
  12. Mansfeld,J. and Ulbrich-Hofmann,R.
    Site-specific and random immobilization of thermolysin-like proteases reflected in the thermal inactivation kinetics
    Biotechnol Appl Biochem (2000) 32, 189-195. PubMed  Europe PubMed DOI
  13. Sookkheo,B., Sinchaikul,S., Phutrakul,S. and Chen,S.T.
    Purification and characterization of the highly thermostable proteases from Bacillus stearothermophilus TLS33
    Protein Expr Purif (2000) 20, 142-151. PubMed  Europe PubMed DOI
    14. 1999
  14. [YEAR:29-6-1999]Mansfeld,J., Vriend,G., Van Den Burg,B., Eijsink,V.G. and Ulbrich-Hofmann,R.
    Probing the unfolding region in a thermolysin-like protease by site-specific immobilization
    Biochemistry (29-6-1999) 38, 8240-8245. PubMed  Europe PubMed DOI
  15. Van Den Burg,B., de Kreij,A., Winkel,C. and Venema,G.
    Hydrolysis of industrial substrates by an extremely stable thermolysin-like protease variant obtained by protein engineering
    Biotechnol Lett (1999) 21, 537-542. DOI
    16. 1998
  16. [YEAR:14-4-1998]Veltman,O.R., Eijsink,V.G., Vriend,G., de Kreij,A., Venema,G. and Van Den Burg,B.
    Probing catalytic hinge bending motions in thermolysin-like proteases by glycine - > alanine mutations
    Biochemistry (14-4-1998) 37, 5305-5311. PubMed  Europe PubMed DOI
  17. [YEAR:14-4-1998]Veltman,O.R., Vriend,G., Berendsen,H.J., Van Den Burg,B., Venema,G. and Eijsink,V.G.
    A single calcium binding site is crucial for the calcium-dependent thermal stability of thermolysin-like proteases
    Biochemistry (14-4-1998) 37, 5312-5319. PubMed  Europe PubMed DOI
    18. 1997
  18. [YEAR:25-4-1997]Mansfeld,J., Vriend,G., Dijkstra,B.W., Veltman,O.R., Van Den Burg,B., Venema,G., Ulbrich-Hofmann,R. and Eijsink,V.G.
    Extreme stabilization of a thermolysin-like protease by an engineered disulfide bond
    J Biol Chem (25-4-1997) 272, 11152-11156. PubMed  Europe PubMed DOI
  19. Nakamura,S., Tanaka,T., Yada,R.Y. and Nakai,S.
    Improving the thermostability of Bacillus stearothermophilus neutral protease by introducing proline into the active site helix
    Protein Eng (1997) 10, 1263-1269. PubMed  Europe PubMed
  20. Veltman,O.R., Vriend,G., Hardy,F., Mansfeld,J., Van Den Burg,B., Venema,G. and Eijsink,V.G.
    Mutational analysis of a surface area that is critical for the thermal stability of thermolysin-like proteases
    Eur J Biochem (1997) 248, 433-440. PubMed  Europe PubMed
  21. Veltman,O.R., Vriend,G., Van Den Burg,B., Hardy,F., Venema,G. and Eijsink,V.G.
    Engineering thermolysin-like proteases whose stability is largely independent of calcium
    FEBS Lett (1997) 405, 241-244. PubMed  Europe PubMed DOI
    22. 1996
  22. Veltman,O.R., Vriend,G., Middelhoven,P.J., Van Den Burg,B., Venema,G. and Eijsink,V.G.
    Analysis of structural determinants of the stability of thermolysin-like proteases by molecular modelling and site-directed mutagenesis
    Protein Eng (1996) 9, 1181-1189. PubMed  Europe PubMed
    23. 1995
  23. Eijsink,V.G., Veltman,O.R., Aukema,W., Vriend,G. and Venema,G.
    Structural determinants of the stability of thermolysin-like proteinases
    Nat Struct Biol (1995) 2, 374-379. PubMed  Europe PubMed DOI
  24. Sidorenkov,I.N., Nosovskaia,E.A. and Kostrov,S.V.
    [Cloning and expression of the Bacillus stearothermophilus neutral proteinase gene in Bacillus subtilis cells]
    Mol Biol (Mosk) (1995) 29, 507-511. PubMed  Europe PubMed
    25. 1994
  25. O'Donohue,M.J., Roques,B.P. and Beaumont,A.
    Cloning and expression in Bacillus subtilis of the npr gene from Bacillus thermoproteolyticus Rokko coding for the thermostable metalloprotease thermolysin
    Biochem J (1994) 300, 599-603. PubMed  Europe PubMed  PMC  EPMC
    26. 1993
  26. Hardy,F., Vriend,G., Veltman,O.R., van der Vinne,B., Venema,G. and Eijsink,V.G.
    Stabilization of Bacillus stearothermophilus neutral protease by introduction of prolines
    FEBS Lett (1993) 317, 89-92. PubMed  Europe PubMed DOI
  27. Vriend,G. and Eijsink,V.
    Prediction and analysis of structure, stability and unfolding of thermolysin-like proteases
    J Comput Aided Mol Des (1993) 7, 367-396. PubMed  Europe PubMed  V
    28. 1992
  28. Eijsink,V.G., Vriend,G., van der Vinne,B., Hazes,B., Van Den Burg,B. and Venema,G.
    Effects of changing the interaction between subdomains on the thermostability of Bacillus neutral proteases
    Proteins (1992) 14, 224-236. PubMed  Europe PubMed DOI
  29. Kubo,M., Mitsuda,Y., Takagi,M. and Imanaka,T.
    Alteration of specific activity and stability of thermostable neutral protease by site-directed mutagenesis
    Appl Environ Microbiol (1992) 58, 3779-3783. PubMed  Europe PubMed  PMC  EPMC
    30. 1991
  30. Eijsink,V.G., Van Den Burg,B. and Venema,G.
    High performance affinity chromatography of Bacillus neutral proteases
    Biotechnol Appl Biochem (1991) 14, 275-283. PubMed  Europe PubMed
    31. 1990
  31. Nishiya,Y. and Imanaka,T.
    Cloning and nucleotide sequences of the Bacillus stearothermophilus neutral protease gene and its transcriptional activator gene
    J Bacteriol (1990) 172, 4861-4869. PubMed  Europe PubMed  PMC  EPMC
    32. 1988
  32. Kubo,M. and Imanaka,T.
    Cloning and nucleotide sequence of the highly thermostable neutral protease gene from Bacillus stearothermophilus
    J Gen Microbiol (1988) 134, 1883-1892. PubMed  Europe PubMed
    33. 1985
  33. Takagi,M., Imanaka,T. and Aiba,S.
    Nucleotide sequence and promoter region for the neutral protease gene from Bacillus stearothermophilus
    J Bacteriol (1985) 163, 824-831. PubMed  Europe PubMed  PMC  EPMC
    34. 1983
  34. Fujii,M., Takagi,M., Imanaka,T. and Aiba,S.
    Molecular cloning of a thermostable neutral protease gene from Bacillus stearothermophilus in a vector plasmid and its expression in Bacillus stearothermophilus and Bacillus subtilis
    J Bacteriol (1983) 154, 831-837. PubMed  Europe PubMed  PMC  EPMC
    35. 1980
  35. Sidler,W. and Zuber,H.
    Isolation procedures for thermostable neutral proteinases produced by Bacillus stearothermophilus
    Eur J Appl Microbiol Biotechnol (1980) 10, 197-209.
    36. 1972
  36. Sidler,W. and Zuber,H.
    Neutral proteases with different thermostabilities from a facultative strain of Bacillus stearothermophilus grown at 40 degrees and at 50 degrees
    FEBS Lett (1972) 25, 292-294. PubMed  Europe PubMed

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