Merops Switch to
Inhibitors

Peptidase

Family Clan SEARCHES BLAST
MEROPS SUBMISSIONS

Other
information

Literature for peptidase C06.001: potato virus Y-type helper component peptidase

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

(Topics flags: S Structure, M Mutation, P Specificity, E Expression, V Review. To select only the references relevant to a single topic, click the link above. See explanation.)

    2025
  1. Fondong,V.N. and Niraula,P.M.<br>Potyvirus HcPro Suppressor of RNA Silencing Induces PVY Superinfection Exclusion in a Strain-Specific Manner<br>Int J Mol Sci (2025) 26 PubMed  Europe PubMed DOI  PMC  EPMC
  2. Jiang,X., Dong,L., Wan,R., Zeng,C. and Yang,T.<br>Investigation of HCPro-Mediated Ethylene Synthesis Pathway Through RNA-Seq Approaches<br>Viruses (2025) 17 PubMed  Europe PubMed DOI  PMC  EPMC
  3. Jin,T., Wang,W., Yu,J., Xiao,Z., Li,Y., Sun,X. and Yang,L.<br>HC-Pro Disrupts miR319-TCP Regulatory Pathways to Induce Sterility in Transgenic Plants<br>Int J Mol Sci (2025) 26 PubMed  Europe PubMed DOI  PMC  EPMC
  4. Pan,Z.J., Wei,W.L., Tran,P.A., Fang,R.Y., Pham,T.H., Bowman,J.L., Chung,C.T., Shen,B.N., Yang,J.T., Chang,H.H., Jane,W.N., Cheng,C.H., Wang,C.C., Wu,H.Y., Hong,S.F., Shang,Q.W., Hu,S.F., Lin,P.C., Wu,F.H., Lin,C.S., Hung,Y.L., Shen,T.L. and Lin,S.S.<br>HC-Pro inhibits HEN1 methyltransferase activity, leading to autophagic degradation of AGO1<br>Nat Commun (2025) 16, 2503-2503. PubMed  Europe PubMed DOI  PMC  EPMC
    5. 2024
  5. Alex,B.G., Zhang,Z.Y., Lasky,D., Garcia-Ruiz,H., Dewberry,R., Allen,C., Halterman,D. and Rakotondrafara,A.M.<br>A single phosphorylatable amino acid residue is essential for the recognition of multiple potyviral HCPro effectors by potato Ny(tbr)<br>Mol Plant Pathol (2024) 25, e70027-e70027. PubMed  Europe PubMed DOI  PMC  EPMC
  6. Parrella,G. and Moury,B.<br>A new point mutation in the HC-Pro of potato virus Y is involved in tobacco vein necrosis<br>PLoS ONE (2024) 19, e0302692-e0302692. PubMed  Europe PubMed DOI  PMC  EPMC
    7. 2023
  7. Cheng,H.W., Lin,T.T., Huang,C.H., Raja,J.A.J. and Yeh,S.D.<br>Modification of papaya ringspot virus HC-Pro to generate effective attenuated mutants for overcoming the problem of strain-specific cross protection<br>Plant Dis (2023) 107, 1757-1768. PubMed  Europe PubMed DOI
  8. Goh,R.P., Xie,X.Y., Lin,Y.C., Cheng,H.W., Raja,J.A.J. and Yeh,S.D.<br>Rapid selection of potyviral cross-protection effective mutants from the local lesion host after nitrous acid mutagenesis<br>Mol Plant Pathol (2023) 24, 973-988. PubMed  Europe PubMed DOI  PMC  EPMC
    9. 2022
  9. Saha,S., Lohmus,A., Dutta,P., Pollari,M. and Makinen,K.<br>Interplay of HCPro and CP in the Regulation of Potato Virus A RNA Expression and Encapsidation<br>Viruses (2022) 14 PubMed  Europe PubMed DOI  PMC  EPMC
    10. 2020
  10. Bao,W., Yan,T., Deng,X. and Wuriyanghan,H.<br>Synthesis of Full-Length cDNA Infectious Clones of Soybean Mosaic Virus and Functional Identification of a Key Amino Acid in the Silencing Suppressor Hc-Pro<br>Viruses (2020) 12 PubMed  Europe PubMed DOI  PMC  EPMC
  11. Tuo,D., Zhou,P., Zhao,G., Yan,P., Tan,D., Li,X. and Shen,W.<br>A double mutation in the conserved motifs of the helper component protease of papaya leaf distortion mosaic virus for the generation of a cross-protective attenuated strain<br>Phytopathology (2020) 110, 187-193. PubMed  Europe PubMed DOI
  12. Yang,T., Qiu,L., Huang,W., Xu,Q., Zou,J., Peng,Q., Lin,H. and Xi,D.<br>Chilli veinal mottle virus HCPro interacts with catalase to facilitate virus infection in Nicotiana tabacum<br>J Exp Bot (2020) 71, 5656-5668. PubMed  Europe PubMed DOI
    13. 2018
  13. Valli,A.A., Gallo,A., Rodamilans,B., Lopez-Moya,J.J. and Garcia,J.A.<br>The HCPro from the potyviridae family: an enviable multitasking helper component that every virus would like to have<br>Mol Plant Pathol (2018) 19, 744-763. PubMed  Europe PubMed DOI
    14. 2015
  14. Tu,Y., Zhang,Z., Li,D., Li,H., Dong,J. and Wang,T.<br>Potato virus Y HC-Pro reduces the ATPase activity of NtMinD, which results in enlarged chloroplasts in HC-Pro transgenic tobacco<br>PLoS ONE (2015) 10, e0136210-e0136210. PubMed  Europe PubMed DOI  PMC  EPMC
    15. 2014
  15. Sahana,N., Kaur,H., Jain,R.K., Palukaitis,P., Canto,T. and Praveen,S.<br>The asparagine residue in the FRNK box of potyviral helper-component protease is critical for its small RNA binding and subcellular localization<br>J Gen Virol (2014) 95, 1167-1177. PubMed  Europe PubMed DOI
  16. Valli,A., Gallo,A., Calvo,M., Perez,J.J. and Garcia,J.A.<br>A novel role of the potyviral helper component proteinase contributes to enhance the yield of viral particles<br>J Virol (2014) 88, 9808-9818. PubMed  Europe PubMed DOI
    17. 2013
  17. Haikonen,T., Rajamaki,M.L., Tian,Y.P. and Valkonen,J.P.<br>Mutation of a short variable region in HCpro protein of potato virus A affects interactions with a microtubule-associated protein and induces necrotic responses in tobacco<br>Mol Plant Microbe Interact (2013) 26, 721-733. PubMed  Europe PubMed DOI
  18. Haikonen,T., Rajamaki,M.L. and Valkonen,J.P.<br>Improved silencing suppression and enhanced heterologous protein expression are achieved using an engineered viral helper component proteinase<br>J Virol Methods (2013) 193, 687-692. PubMed  Europe PubMed DOI
    19. 2012
  19. Atsumi,G., Nakahara,K.S., Wada,T.S., Choi,S.H., Masuta,C. and Uyeda,I.<br>Heterologous expression of viral suppressors of RNA silencing complements virulence of the HC-Pro mutant of clover yellow vein virus in pea<br>Arch Virol (2012) 157, 1019-1028. PubMed  Europe PubMed DOI
  20. Faurez,F., Baldwin,T., Tribodet,M. and Jacquot,E.<br>Identification of new potato virus Y (PVY) molecular determinants for the induction of vein necrosis in tobacco<br>Mol Plant Pathol (2012) 13, 948-959. PubMed  Europe PubMed DOI  M
  21. Krczal,G. and Wassenegger,M.<br>Potyvirus helper component proteinase<br>[ISSN:978-0-12-407743-0] (2012) 3, 2187-2192. DOI
  22. Lim,H.-S., Jang,C.-Y., Nam,J., Li,M., Hong,J.-S., Bae,H., Ju,H.-J., Kim,H.G., Ford,R.E. and Domier,L.L.<br>Characterization of the in vitro activities of the P1 and helper component proteases of Soybean mosaic virus strain G2 and Tobacco vein mottling virus<br>Plant Pathol J (2012) 28, 197-201. DOI
    23. 2011
  23. Boonrod,K., Fullgrabe,M.W., Krczal,G. and Wassenegger,M.<br>Analysis of the autoproteolytic activity of the recombinant helper component proteinase from zucchini yellow mosaic virus<br>Biol Chem (2011) 392, 937-945. PubMed  Europe PubMed DOI
  24. Fuellgrabe,M.W., Boonrod,K., Jamous,R., Moser,M., Shiboleth,Y., Krczal,G. and Wassenegger,M.<br>Expression, purification and functional characterization of recombinant Zucchini yellow mosaic virus HC-Pro<br>Protein Expr Purif (2011) 75, 40-45. PubMed  Europe PubMed DOI  E
  25. Guo,B., Lin,J. and Ye,K.<br>Structure of the autocatalytic cysteine protease domain of potyvirus helper-component proteinase<br>J Biol Chem (2011) 286, 21937-21943. PubMed  Europe PubMed DOI  S
  26. Zheng,H., Yan,F., Lu,Y., Sun,L., Lin,L., Cai,L., Hou,M. and Chen,J.<br>Mapping the self-interacting domains of TuMV HC-Pro and the subcellular localization of the protein<br>Virus Genes (2011) 42, 110-116. PubMed  Europe PubMed DOI
    27. 2010
  27. Fullgrabe,M., Boonrod,K., Jamous,R., Moser,M., Shiboleth,Y., KrczalK. and Wassenegger,M.<br>Expression, purification and functional characterization of recombinant Zucchini yellow mosaic virus HC-Pro<br>Protein Expr Purif (2010) 75, 40-45.  E
  28. Mangrauthia,S.K., Singh,P. and Praveen,S.<br>Genomics of helper component proteinase reveals effective strategy for papaya ringspot virus resistance<br>Mol Biotechnol (2010) 44, 22-29. PubMed  Europe PubMed DOI
  29. Seo,J.K., Kang,S.H., Seo,B.Y., Jung,J.K. and Kim,K.H.<br>Mutational analysis of interaction between coat protein and helper component-proteinase of Soybean mosaic virus involved in aphid transmission<br>Mol Plant Pathol (2010) 11, 265-276. PubMed  Europe PubMed DOI
  30. Wu,H.W., Lin,S.S., Chen,K.C., Yeh,S.D. and Chua,N.H.<br>Discriminating mutations of HC-Pro of Zucchini yellow mosaic virus with differential effects on small RNA pathways involved in viral pathogenicity and symptom development<br>Mol Plant Microbe Interact (2010) 23, 17-28. PubMed  Europe PubMed DOI  M
  31. You,Y. and Shirako,Y.<br>Bymovirus reverse genetics: requirements for RNA2-encoded proteins in systemic infection<br>Mol Plant Pathol (2010) 11, 383-394. PubMed  Europe PubMed DOI
    32. 2009
  32. Yap,Y.K., Duangjit,J. and Panyim,S.<br>N-terminal of Papaya ringspot virus type-W (PRSV-W) helper component proteinase (HC-Pro) is essential for PRSV systemic infection in zucchini<br>Virus Genes (2009) 38, 461-467. PubMed  Europe PubMed DOI
    33. 2008
  33. Yambao,M.L., Yagihashi,H., Sekiguchi,H., Sekiguchi,T., Sasaki,T., Sato,M., Atsumi,G., Tacahashi,Y., Nakahara,K.S. and Uyeda,I.<br>Point mutations in helper component protease of clover yellow vein virus are associated with the attenuation of RNA-silencing suppression activity and symptom expression in broad bean<br>Arch Virol (2008) 153, 105-115. PubMed  Europe PubMed DOI
    34. 2007
  34. Dombrovsky,A., Gollop,N., Chen,S., Chejanovsky,N. and Raccah,B.<br>In vitro association between the helper component-proteinase of zucchini yellow mosaic virus and cuticle proteins of Myzus persicae<br>J Gen Virol (2007) 88, 1602-1610. PubMed  Europe PubMed DOI
  35. Lim,H.S., Ko,T.S., Hobbs,H.A., Lambert,K.N., Yu,J.M., McCoppin,N.K., Korban,S.S., Hartman,G.L. and Domier,L.L.<br>Soybean mosaic virus helper component-protease alters leaf morphology and reduces seed production in transgenic soybean plants<br>Phytopathology (2007) 97, 366-372. PubMed  Europe PubMed DOI
  36. Lin,S.S., Wu,H.W., Jan,F.J., Hou,R.F. and Yeh,S.D.<br>Modifications of the helper component-protease of Zucchini yellow mosaic virus for generation of attenuated mutants for cross protection against severe infection<br>Phytopathology (2007) 97, 287-296. PubMed  Europe PubMed DOI
  37. Shiboleth,Y.M., Haronsky,E., Leibman,D., Arazi,T., Wassenegger,M., Whitham,S.A., Gaba,V. and Gal-On,A.<br>The conserved FRNK box in HC-Pro, a plant viral suppressor of gene silencing, is required for small RNA binding and mediates symptom development<br>J Virol (2007) 81, 13135-13148. PubMed  Europe PubMed DOI  PMC  EPMC
  38. Varrelmann,M., Maiss,E., Pilot,R. and Palkovics,L.<br>Use of pentapeptide-insertion scanning mutagenesis for functional mapping of the plum pox virus helper component proteinase suppressor of gene silencing<br>J Gen Virol (2007) 88, 1005-1015. PubMed  Europe PubMed DOI
    39. 2005
  39. Ballut,L., Drucker,M., Pugniere,M., Cambon,F., Blanc,S., Roquet,F., Candresse,T., Schmid,H.P., Nicolas,P., Gall,O.L. and Badaoui,S.<br>HcPro, a multifunctional protein encoded by a plant RNA virus, targets the 20S proteasome and affects its enzymic activities<br>J Gen Virol (2005) 86, 2595-2603. PubMed  Europe PubMed DOI
  40. Gonzalez-Jara,P., Atencio,F.A., Martinez-Garcia,B., Barajas,D., Tenllado,F. and Diaz-Ruiz,J.R.<br>A single amino acid mutation in the Plum pox virus helper component-proteinase gene abolishes both synergistic and rna silencing suppression activities<br>Phytopathology (2005) 95, 894-901. PubMed  Europe PubMed DOI
  41. Ruiz-Ferrer,V., Boskovic,J., Alfonso,C., Rivas,G., Llorca,O., Lopez-Abella,D. and Lopez-Moya,J.J.<br>Structural analysis of tobacco etch potyvirus HC-pro oligomers involved in aphid transmission<br>J Virol (2005) 79, 3758-3765. PubMed  Europe PubMed DOI  PMC  EPMC  E
    42. 2004
  42. Verchot-Lubicz,J.<br>Potyvirus helper component proteinase<br>[ISSN:0-12-079610-4] (2004) 2, 1262-1264.  V
    43. 2003
  43. Plisson,C., Drucker,M., Blanc,S., German-Retana,S., Le Gall,O., Thomas,D. and Bron,P.<br>Structural characterization of HC-Pro, a plant virus multifunctional protein<br>J Biol Chem (2003) 278, 23753-23761. PubMed  Europe PubMed DOI
    44. 2002
  44. Mlotshwa,S., Verver,J., Sithole-Niang,I., Gopinath,K., Carette,J., van Kammen,A. and Wellink,J.<br>Subcellular location of the helper component-proteinase of Cowpea aphid-borne mosaic virus<br>Virus Genes (2002) 25, 207-216. PubMed  Europe PubMed DOI
  45. Roudet-Tavert,G., German-Retana,S., Delaunay,T., Delecolle,B., Candresse,T. and Le Gall,O.<br>Interaction between potyvirus helper component-proteinase and capsid protein in infected plants<br>J Gen Virol (2002) 83, 1765-1770. PubMed  Europe PubMed
    46. 2001
  46. Mallory,A.C., Ely,L., Smith,T.H., Marathe,R., Anandalakshmi,R., Fagard,M., Vaucheret,H., Pruss,G., Bowman,L. and Vance,V.B.<br>HC-Pro suppression of transgene silencing eliminates the small RNAs but not transgene methylation or the mobile signal<br>Plant Cell (2001) 13, 571-583. PubMed  Europe PubMed DOI  PMC  EPMC
  47. [YEAR:10-5-2001]Savenkov,E.I. and Valkonen,J.P.<br>Potyviral helper-component proteinase expressed in transgenic plants enhances titers of Potato leaf roll virus but does not alleviate its phloem limitation<br>Virology (10-5-2001) 283, 285-293. PubMed  Europe PubMed DOI
    48. 2000
  48. Anandalakshmi,R., Marathe,R., Ge,X., Herr,J.M., Jr., Mau,C., Mallory,A., Pruss,G., Bowman,L. and Vance,V.B.<br>A calmodulin-related protein that suppresses posttranscriptional gene silencing in plants<br>Science (2000) 290, 142-144. PubMed  Europe PubMed DOI
  49. Gal-On,A.<br>A point mutation in the FRNK motif of the potyvirus helper component-protease gene alters symptom expression in cucurbits and elicits protection against the severe homologous virus<br>Phytopathology (2000) 90, 467-473. PubMed  Europe PubMed DOI
    50. 1999
  50. Andrejeva,J., Puurand,U., Merits,A., Rabenstein,F., Jarvekulg,L. and Valkonen,J.P.<br>Potyvirus helper component-proteinase and coat protein (CP) have coordinated functions in virus-host interactions and the same CP motif affects virus transmission and accumulation<br>J Gen Virol (1999) 80, 1133-1139. PubMed  Europe PubMed
  51. Guo,D., Merits,A. and Saarma,M.<br>Self-association and mapping of interaction domains of helper component-proteinase of potato A potyvirus<br>J Gen Virol (1999) 80, 1127-1131. PubMed  Europe PubMed
  52. Ohshima,K.<br>Accumulation of helper component/proteinase and coat protein of turnip mosaic virus in intact plants<br>Acta Virol (1999) 43, 31-37. PubMed  Europe PubMed
  53. Wang,R.Y. and Pirone,T.P.<br>Purification and characterization of turnip mosaic virus helper component protein<br>Phytopathology (1999) 89, 564-567. PubMed  Europe PubMed DOI
    54. 1998
  54. [YEAR:27-10-1998]Anandalakshmi,R., Pruss,G.J., Ge,X., Marathe,R., Mallory,A.C., Smith,T.H. and Vance,V.B.<br>A viral suppressor of gene silencing in plants<br>Proc Natl Acad Sci U S A (27-10-1998) 95, 13079-13084. PubMed  Europe PubMed  PMC  EPMC
  55. [YEAR:16-11-1998]Brigneti,G., Voinnet,O., Li,W.X., Ji,L.H., Ding,S.W. and Baulcombe,D.C.<br>Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana<br>EMBO J (16-11-1998) 17, 6739-6746. PubMed  Europe PubMed DOI  PMC  EPMC
  56. Kasschau,K.D. and Carrington,J.C.<br>A counterdefensive strategy of plant viruses: suppression of posttranscriptional gene silencing<br>Cell (1998) 95, 461-470. PubMed  Europe PubMed
    57. 1997
  57. Rojas,M.R., Zerbini,F.M., Allison,R.F., Gilbertson,R.L. and Lucas,W.J.<br>Capsid protein and helper component-proteinase function as potyvirus cell-to-cell movement proteins<br>Virology (1997) 237, 283-295. PubMed  Europe PubMed DOI
  58. Ryan,M.D. and Flint,M.<br>Virus-encoded proteinases of the picornavirus super-group<br>J Gen Virol (1997) 78, 699-723. PubMed  Europe PubMed
    59. 1995
  59. Cronin,S., Verchot,J., Haldeman-Cahill,R., Schaad,M.C. and Carrington,J.C.<br>Long-distance movement factor: a transport function of the potyvirus helper component proteinase<br>Plant Cell (1995) 7, 549-559. PubMed  Europe PubMed DOI  PMC  EPMC
    60. 1993
  60. Dolja,V.V., Herndon,K.L., Pirone,T.P. and Carrington,J.C.<br>Spontaneous mutagenesis of a plant potyvirus genome after insertion of a foreign gene<br>J Virol (1993) 67, 5968-5975. PubMed  Europe PubMed  PMC  EPMC
  61. Dougherty,W.G. and Semler,B.L.<br>Expression of virus-encoded proteinases: functional and structural similarities with cellular enzymes<br>Microbiol Rev (1993) 57, 781-822. PubMed  Europe PubMed  PMC  EPMC
  62. Stram,Y., Chetsrony,A., Karchi,H., Karchi,M., Edelbaum,O., Vardi,E., Livneh,O. and Sela,I.<br>Expression of the "helper component" protein of potato virus Y (PVY) in E. coli: possible involvement of a third protease<br>Virus Genes (1993) 7, 151-156. PubMed  Europe PubMed  E
    63. 1992
  63. Atreya,C.D., Atreya,P.L., Thornbury,D.W. and Pirone,T.P.<br>Site-directed mutations in the potyvirus HC-Pro gene affect helper component activity, virus accumulation, and symptom expression in infected tobacco plants<br>Virology (1992) 191, 106-111. PubMed  Europe PubMed DOI
  64. Carrington,J.C. and Herndon,K.L.<br>Characterization of the potyviral HC-Pro autoproteolytic cleavage site<br>Virology (1992) 187, 308-315. PubMed  Europe PubMed DOI  P
    65. 1991
  65. Choi,G.H., Pawlyk,D.M. and Nuss,D.L.<br>The autocatalytic protease p29 encoded by a hypovirulence-associated virus of the chestnut blight fungus resembles the potyvirus-encoded protease HC-Pro<br>Virology (1991) 183, 747-752. PubMed  Europe PubMed DOI
  66. Koonin,E.V., Choi,G.H., Nuss,D.L., Shapira,R. and Carrington,J.C.<br>Evidence for common ancestry of a chestnut blight hypovirulence-associated double-stranded RNA and a group of positive-strand RNA plant viruses<br>Proc Natl Acad Sci U S A (1991) 88, 10647-10651. PubMed  Europe PubMed  PMC  EPMC
    67. 1989
  67. Carrington,J.C., Cary,S.M., Parks,T.D. and Dougherty,W.G.<br>A second proteinase encoded by a plant potyvirus genome<br>EMBO J (1989) 8, 365-370. PubMed  Europe PubMed  PMC  EPMC
  68. Carrington,J.C., Freed,D.D. and Sanders,T.C.<br>Autocatalytic processing of the potyvirus helper component proteinase in Escherichia coli and in vitro<br>J Virol (1989) 63, 4459-4463. PubMed  Europe PubMed  PMC  EPMC
  69. Oh,C.S. and Carrington,J.C.<br>Identification of essential residues in potyvirus proteinase HC-Pro by site-directed mutagenesis<br>Virology (1989) 173, 692-699. PubMed  Europe PubMed DOI
    70. 1985
  70. Thornbury,D.W., Hellmann,G.M., Rhoads,R.E. and Pirone,T.P.<br>Purification and characterization of potyvirus helper component<br>Virology (1985) 144, 260-267. PubMed  Europe PubMed

feedback
Page created 8-September-2023

EMBL-EBI logo

© 2025 EBI
Terms of use