Merops Switch to
Inhibitors

Peptidase

Family Clan SEARCHES BLAST
MEROPS SUBMISSIONS

Other
information

Literature for peptidase C04.004: tobacco etch virus NIa peptidase

Summary Alignment Sequences Sequence features Distribution Structure Literature Substrates

(Topics flags: S Structure, A Assay, P Specificity, L Localization, E Expression, U Therapeutic, V Review. To select only the references relevant to a single topic, click the link above. See explanation.)

    2025
  1. Davis,S.M., Romig,B.L., Abe,A.A. and Loening,N.M.
    An improved variant of tobacco etch virus (TEV) protease that does not need reducing agents
    Protein Sci34, e70049-e70049. PubMed  Europe PubMed DOI
    2. 2024
  2. Beaumont,L.P., Mehalko,J., Johnson,A., Wall,V.E. and Esposito,D.
    Unexpected tobacco etch virus (TEV) protease cleavage of recombinant human proteins
    Protein Expr Purif220, 106488-106488. PubMed  Europe PubMed DOI
  3. Cui,M., Lee,S., Ban,S.H., Ryu,J.R., Shen,M., Yang,S.H., Kim,J.Y., Choi,S.K., Han,J., Kim,Y., Han,K., Lee,D., Sun,W., Kwon,H.B. and Lee,D.
    A single-component, light-assisted uncaging switch for endoproteolytic release
    Nat Chem Biol20, 353-364. PubMed  Europe PubMed DOI
  4. Devoy,C., Flores Bueso,Y., Buckley,S., Walker,S. and Tangney,M.
    Synthetic protein protease sensor platform
    Front Bioeng Biotechnol12, 1347953-1347953. PubMed  Europe PubMed DOI
  5. Golda,M., Hoffka,G., Cherry,S., Tropea,J.E., Lountos,G.T., Waugh,D.S., Wlodawer,A., Tozser,J. and Motyan,J.A.
    P1' specificity of the S219V/R203G mutant tobacco etch virus protease
    Proteins92, 1085-1096-1096. PubMed  Europe PubMed DOI
  6. Lan,T., Peng,C., Yao,X., Chan,R.S.T., Wei,T., Rupanya,A., Radakovic,A., Wang,S., Chen,S., Lovell,S., Snyder,S.A., Bogyo,M. and Dickinson,B.C.
    Discovery of Thioether-Cyclized Macrocyclic Covalent Inhibitors by mRNA Display
    J Am Chem Soc146, 24053-24060. PubMed  Europe PubMed DOI
  7. Martinusen,S.G., Slaton,E.W., Nelson,S.E., Pulgar,M.A., Besu,J.T., Simas,C.F. and Denard,C.A.
    Modular and integrative activity reporters enhance biochemical studies in the yeast ER
    Protein Eng Des Sel37, PubMed  Europe PubMed DOI
  8. Messing,S., Barnhart,K., Drew,M., Granato-Guerrero,N., Grose,C., Higgins,B., Hong,M., Hull,J., Perkins,S., Poon,I., Ramakrishnan,N., Seabolt,A., Taylor,T., Wall,V.E., Wright,N., Gillette,W. and Esposito,D.
    Improvements in large-scale production of Tobacco Etch Virus protease
    Protein Expr Purif106648-106648. PubMed  Europe PubMed DOI
  9. Shahriari,M., Shafiee,F., Moazen,F. and Mohammad Sadeghi,H.M.
    Optimization of the Production of Soluble Recombinant TEV Protease in Two E. coli Strains
    Avicenna J Med Biotechnol16, 279-283. PubMed  Europe PubMed DOI
  10. Sumida,K.H., Nunez-Franco,R., Kalvet,I., Pellock,S.J., Wicky,B.I.M., Milles,L.F., Dauparas,J., Wang,J., Kipnis,Y., Jameson,N., Kang,A., de la Cruz,J., Sankaran,B., Bera,A.K., Jimenez-Oses,G. and Baker,D.
    Improving Protein Expression, Stability, and Function with ProteinMPNN
    J Am Chem Soc146, 2054-2061. PubMed  Europe PubMed DOI
  11. Wang,J., Xu,Y., Wang,X., Li,J. and Hua,Z.
    Mechanism of Mutation-Induced Effects on the Catalytic Function of TEV Protease: A Molecular Dynamics Study
    Molecules29, PubMed  Europe PubMed DOI
  12. Wang,Y., Zheng,J., Wen,F., Tu,B. and Cui,L.
    Novel gnd_v2 Fusion Tag and Engineered TEV Protease Enable Efficient Production of Brazzein
    J Microbiol Biotechnol34, 1-11. PubMed  Europe PubMed DOI
    13. 2023
  13. Fourest-Lieuvin,A., Vinit,A., Blot,B., Perrot,A., Denarier,E., Saudou,F. and Arnal,I.
    Controlled Tau Cleavage in Cells Reveals Abnormal Localizations of Tau Fragments
    Neuroscience518, 162-177. PubMed  Europe PubMed DOI
  14. Meister,S.W., Parks,L., Kolmar,L., Mestre Borras,A., Stahl,S. and Lofblom,J.
    Engineering of TEV protease variants with redesigned substrate specificity
    Biotechnol Je2200625-e2200625. PubMed  Europe PubMed DOI
  15. Parida,P.P., Saraswathi,D., Mopidevi,S.M.V. and Raran-Kurussi,S.
    Advancing large-scale production of TEV protease through an innovative NT* tag-based fusion construct
    Curr Res Struct Biol6, 100106-100106. PubMed  Europe PubMed DOI
  16. Winckler,L.I. and Dissmeyer,N.
    TEV protease cleavage in generation of artificial substrate proteins bearing neo-N-termini
    Methods Enzymol686, 125-141. PubMed  Europe PubMed DOI
  17. Zheng,M. and Gao,J.
    Phage Display of Two Distinct Warheads to Inhibit Challenging Proteins
    ACS Chem Biol18, 2259-2266. PubMed  Europe PubMed DOI
    18. 2022
  18. Enriquez-Flores,S., De la Mora-De la Mora, Flores-Lopez,L.A., Cabrera,N., Fernandez-Lainez,C., Hernandez-Alcantara,G., Guerrero-Beltran,C.E., Lopez-Velazquez,G. and Garcia-Torres,I.
    Improved yield, stability, and cleavage reaction of a novel tobacco etch virus protease mutant
    Appl Microbiol Biotechnol106, 1475-1492. PubMed  Europe PubMed DOI  V
  19. Paththamperuma,C. and Page,R.C.
    Fluorescence dequenching assay for the activity of TEV protease
    Anal Biochem659, 114954-114954. PubMed  Europe PubMed DOI
  20. Renna,P., Ripoli,C., Dagliyan,O., Pastore,F., Rinaudo,M., Re,A., Paciello,F. and Grassi,C.
    Engineering a switchable single-chain TEV protease to control protein maturation in living neurons
    Bioeng Transl Med7, e10292-e10292. PubMed  Europe PubMed DOI
  21. Zlobin,A. and Golovin,A.
    Between Protein Fold and Nucleophile Identity: Multiscale Modeling of the TEV Protease Enzyme-Substrate Complex
    ACS Omega7, 40279-40292. PubMed  Europe PubMed DOI
    22. 2021
  22. Alonso-Gardon,M. and Estevez,R.
    Split-Tobacco Etch Virus (Split-TEV) Method in G Protein-Coupled Receptor Interacting Proteins
    Methods Mol Biol2268, 223-232. PubMed  Europe PubMed DOI
  23. Bayar,E., Ren,Y., Chen,Y., Hu,Y., Zhang,S., Yu,X. and Fan,J.
    Construction, investigation and application of the TEV protease variants with the improved oxidative stability
    J Microbiol Biotechnol31, PubMed  Europe PubMed DOI
  24. Denard,C.A., Paresi,C., Yaghi,R., McGinnis,N., Bennett,Z., Yi,L., Georgiou,G. and Iverson,B.L.
    YESS 2.0, a Tunable Platform for Enzyme Evolution, Yields Highly Active TEV Protease Variants
    ACS Synth Biol10, 63-71. PubMed  Europe PubMed DOI
    25. 2020
  25. Brungardt,J., Govind,R. and Trick,H.N.
    A simplified method for producing laboratory grade recombinant TEV protease from E. coli
    Protein Expr Purif174, 105662-105662. PubMed  Europe PubMed DOI  E
  26. Chen,S., Lovell,S., Lee,S., Fellner,M., Mace,P.D. and Bogyo,M.
    Identification of highly selective covalent inhibitors by phage display
    Nat Biotechnol PubMed  Europe PubMed DOI
  27. Chung,H.K. and Lin,M.Z.
    On the cutting edge: protease-based methods for sensing and controlling cell biology
    Nat Methods PubMed  Europe PubMed DOI  V
  28. Cupo,R.R. and Shorter,J.
    Expression and Purification of Recombinant Skd3 (Human ClpB) Protein and Tobacco Etch Virus (TEV) Protease from Escherichia coli
    Bio Protoc10, e3858-e3858. PubMed  Europe PubMed DOI
  29. Holt,B.A. and Kwong,G.A.
    Protease circuits for processing biological information
    Nat Commun11, 5021-5021. PubMed  Europe PubMed DOI
  30. Lakshmanan,A., Jin,Z., Nety,S.P., Sawyer,D.P., Lee-Gosselin,A., Malounda,D., Swift,M.B., Maresca,D. and Shapiro,M.G.
    Acoustic biosensors for ultrasound imaging of enzyme activity
    Nat Chem Biol PubMed  Europe PubMed DOI
  31. Mohammadian,N., Mohammadian,H., Moazen,F., Pakdel,M.H., Jahanian-Najafabadi,A. and Mir Mohammad Sadeghi,H.
    Optimization of solvent media to solubilize TEV protease using response surface method
    Res Pharm Sci15, 331-339. PubMed  Europe PubMed DOI
  32. Nam,H., Hwang,B.J., Choi,D.Y., Shin,S. and Choi,M.
    Tobacco etch virus (TEV) protease with multiple mutations to improve solubility and reduce self-cleavage exhibits enhanced enzymatic activity
    FEBS Open Bio10, 619-626. PubMed  Europe PubMed DOI  E
  33. Norris,J.L., Patel,T., Dasari,A.K.R., Cope,T.A., Lim,K.H. and Hughes,R.M.
    Covalent and non-covalent strategies for the immobilization of Tobacco Etch Virus protease (TEVp) on superparamagnetic nanoparticles
    J Biotechnol322, 1-9. PubMed  Europe PubMed DOI
  34. Sanchez,M.I. and Ting,A.Y.
    Directed evolution improves the catalytic efficiency of TEV protease
    Nat Methods17, 167-174. PubMed  Europe PubMed DOI  L
  35. Sanchez,M.I., Nguyen,Q.A., Wang,W., Soltesz,I. and Ting,A.Y.
    Transcriptional readout of neuronal activity via an engineered Ca(2+)-activated protease
    Proc Natl Acad Sci U S A117, 33186-33196. PubMed  Europe PubMed DOI
    36. 2018
  36. Nautiyal,K. and Kuroda,Y.
    A SEP tag enhances the expression, solubility and yield of recombinant TEV protease without altering its activity
    N Biotechnol42, 77-84. PubMed  Europe PubMed DOI  E
  37. O'Neill,B.K. and Laughlin,S.T.
    Neuronal calcium recording with an engineered TEV protease
    ACS Chem Biol13, 1159-1164. PubMed  Europe PubMed DOI
    38. 2017
  38. Chen,Y., Li,Q., Yang,J. and Xie,H.
    Promoting tag removal of a MBP-fused integral membrane protein by TEV protease
    Appl Biochem Biotechnol181, 939-947. PubMed  Europe PubMed DOI
  39. Packer,M.S., Rees,H.A. and Liu,D.R.
    Phage-assisted continuous evolution of proteases with altered substrate specificity
    Nat Commun8, 956-956. PubMed  Europe PubMed DOI
  40. Raran-Kurussi,S., Cherry,S., Zhang,D. and Waugh,D.S.
    Removal of affinity tags with TEV protease
    Methods Mol Biol1586, 221-230. PubMed  Europe PubMed DOI
  41. Ren,S., Zhu,G. and Du,L.
    Study on the impacts of Co2+ on the tobacco etch virus protease
    Chin J App Eviron Biol23, 113-116. DOI
    42. 2016
  42. Cesaratto,F., Burrone,O.R. and Petris,G.
    Tobacco etch virus protease: a shortcut across biotechnologies
    J Biotechnol231, 239-249. PubMed  Europe PubMed DOI
  43. He,Y.H., Li,Y.M. and Chen,Y.X.
    Phosphorylation regulates proteolytic efficiency of TEV protease detected by a 5(6)-carboxyfluorescein-pyrene based fluorescent sensor
    Talanta150, 340-345. PubMed  Europe PubMed DOI
    44. 2015
  44. Cesaratto,F., Lopez-Requena,A., Burrone,O.R. and Petris,G.
    Engineered tobacco etch virus (TEV) protease active in the secretory pathway of mammalian cells
    J Biotechnol212, 159-166. PubMed  Europe PubMed DOI
  45. Gerhardt,K. and Tabor,J.
    Optogenetic regulation of proteolysis using an engineered light-switchable split Tev protease
    Synth Biol : Eng , Evol , Des Conf , SEED2, 804-805.
  46. Shafee,T., Gatti-Lafranconi,P., Minter,R. and Hollfelder,F.
    Handicap-recover evolution leads to a chemically versatile, nucleophile-permissive protease
    Chembiochem16, 1866-1869. PubMed  Europe PubMed DOI
  47. Zhu,G.-F., Ren,S.-Y., Xi,L., Du,L.-F. and Zhu,X.-F.
    Temperature induced structural transitions from native to unfolded aggregated states of tobacco etch virus protease
    J Mol Struct1082, 80-90. DOI
    48. 2014
  48. Fang,J., Zou,L., Zhou,X., Cheng,B. and Fan,J.
    Synonymous rare arginine codons and tRNA abundance affect protein production and quality of TEV protease variant
    PLoS ONE9, e112254-e112254. PubMed  Europe PubMed DOI  E
    49. 2013
  49. Fang,J., Chen,L., Cheng,B. and Fan,J.
    Engineering soluble tobacco etch virus protease accompanies the loss of stability
    Protein Expr Purif92, 29-35. PubMed  Europe PubMed DOI
  50. Raran-Kurussi,S., Tozser,J., Cherry,S., Tropea,J.E. and Waugh,D.S.
    Differential temperature dependence of tobacco etch virus and rhinovirus 3C proteases
    Anal Biochem436, 142-144. PubMed  Europe PubMed DOI
  51. Renicke,C., Spadaccini,R. and Taxis,C.
    A tobacco etch virus protease with increased substrate tolerance at the P1' position
    PLoS ONE8, e67915-e67915. PubMed  Europe PubMed DOI
  52. Wang,J.-Z., Ren,S.-Y., Zhu,G.-F., Xi,L., Han,Y.-G., Luo,Y. and Du,L.-F.
    Hg2+ interference with the structure of tobacco etch virus protease (TEVp) and its implications for biological engineering
    J Mol Struct1052, 85-92. DOI
  53. Wang,Y., Zhu,G.F., Ren,S.Y., Han,Y.G., Luo,Y. and Du,L.F.
    Insight into the structural stability of wild type and mutants of the tobacco etch virus protease with molecular dynamics simulations
    J Mol Model19, 4865-4875. PubMed  Europe PubMed DOI
  54. Yi,L., Gebhard,M.C., Li,Q., Taft,J.M., Georgiou,G. and Iverson,B.L.
    Engineering of TEV protease variants by yeast ER sequestration screening (YESS) of combinatorial libraries
    Proc Natl Acad Sci U S A110, 7229-7234. PubMed  Europe PubMed DOI
    55. 2012
  55. Han,J.S., Kim,D.H. and Choi,K.Y.
    Potyvirus NIa protease
    [ISSN:978-0-12-407743-0]3, 2427-2432. DOI
  56. Kim,T.K., Han,H.E., Kim,H., Lee,J.E., Choi,D., Park,W.J. and Han,P.L.
    Expression of the plant viral protease NIa in the brain of a mouse model of Alzheimer's disease mitigates Abeta pathology and improves cognitive function
    Exp Mol Med44, 740-748. PubMed  Europe PubMed DOI  U
  57. Miladi,B., Marjou,A.E., Boeuf,G., Bouallagui,H., Dufour,F., Di Martino,P. and Elm'selmi,A.
    Oriented immobilization of the tobacco etch virus protease for the cleavage of fusion proteins
    J Biotechnol158, 97-103. PubMed  Europe PubMed DOI
  58. Sun,C., Liang,J., Shi,R., Gao,X., Zhang,R., Hong,F., Yuan,Q. and Wang,S.
    Tobacco etch virus protease retains its activity in various buffers and in the presence of diverse additives
    Protein Expr Purif82, 226-231. PubMed  Europe PubMed DOI
  59. Verhoeven,K.D., Altstadt,O.C. and Savinov,S.N.
    Intracellular detection and evolution of site-specific proteases using a genetic selection system
    Appl Biochem Biotechnol166, 1340-1354. PubMed  Europe PubMed DOI
  60. Wei,L., Cai,X., Qi,Z., Rong,L., Cheng,B. and Fan,J.
    In vivo and in vitro characterization of TEV protease mutants
    Protein Expr Purif83, 157-163. PubMed  Europe PubMed DOI  E
    61. 2011
  61. Djannatian,M.S., Galinski,S., Fischer,T.M. and Rossner,M.J.
    Studying G protein-coupled receptor activation using split-tobacco etch virus assays
    Anal Biochem412, 141-152. PubMed  Europe PubMed DOI
  62. Kostallas,G., Lofdahl,P.A. and Samuelson,P.
    Substrate profiling of tobacco etch virus protease using a novel fluorescence-assisted whole-cell assay
    PLoS ONE6, e16136-e16136. PubMed  Europe PubMed DOI  P
  63. Miladi,B., Bouallagui,H., Dridi,C., El Marjou,A., Boeuf,G., Di Martino,P., Dufour,F. and Elm'selmi,A.
    A new tagged-TEV protease: construction, optimisation of production, purification and test activity
    Protein Expr Purif75, 75-82. PubMed  Europe PubMed DOI
    64. 2010
  64. Boulware,K.T., Jabaiah,A. and Daugherty,P.S.
    Evolutionary optimization of peptide substrates for proteases that exhibit rapid hydrolysis kinetics
    Biotechnol Bioeng106, 339-346. PubMed  Europe PubMed DOI  P
    65. 2009
  65. Puhl,A.C., Giacomini,C., Irazoqui,G., Batista-Viera,F., Villarino,A. and Terenzi,H.
    Covalent immobilization of tobacco-etch-virus NIa protease: a useful tool for cleavage of the histidine tag of recombinant proteins
    Biotechnol Appl Biochem53, 165-174. PubMed  Europe PubMed DOI
  66. Wu,X., Wu,D., Lu,Z., Chen,W., Hu,X. and Ding,Y.
    A novel method for high-level production of TEV protease by superfolder GFP tag
    J Biomed Biotechnol2009, 591923-591923. PubMed  Europe PubMed DOI  E
    67. 2008
  67. Harder,B., Schomburg,A., Pflanz,R., Kustner,K., Gerlach,N. and Schuh,R.
    TEV protease-mediated cleavage in Drosophila as a tool to analyze protein functions in living organisms
    Biotechniques44, 765-772. PubMed  Europe PubMed DOI
  68. Lundback,A.K., van den Berg,S., Hebert,H., Berglund,H. and Eshaghi,S.
    Exploring the activity of tobacco etch virus protease in detergent solutions
    Anal Biochem382, 69-71. PubMed  Europe PubMed DOI
  69. Pauli,A., Althoff,F., Oliveira,R.A., Heidmann,S., Schuldiner,O., Lehner,C.F., Dickson,B.J. and Nasmyth,K.
    Cell-type-specific TEV protease cleavage reveals cohesin functions in Drosophila neurons
    Dev Cell14, 239-251. PubMed  Europe PubMed DOI
  70. Wehr,M.C., Reinecke,L., Botvinnik,A. and Rossner,M.J.
    Analysis of transient phosphorylation-dependent protein-protein interactions in living mammalian cells using split-TEV
    BMC Biotechnol8, 55-55. PubMed  Europe PubMed DOI
    71. 2007
  71. Blommel,P.G. and Fox,B.G.
    A combined approach to improving large-scale production of tobacco etch virus protease
    Protein Expr Purif55, 53-68. PubMed  Europe PubMed DOI  E
  72. Cabrita,L.D., Gilis,D., Robertson,A.L., Dehouck,Y., Rooman,M. and Bottomley,S.P.
    Enhancing the stability and solubility of TEV protease using in silico design
    Protein Sci16, 2360-2367. PubMed  Europe PubMed DOI
  73. Eser,M., Henrichs,T., Boyd,D. and Ehrmann,M.
    Target-directed proteolysis in vivo
    Methods Enzymol421, 68-83. PubMed  Europe PubMed DOI
  74. Fang,L., Jia,K.Z., Tang,Y.L., Ma,D.Y., Yu,M. and Hua,Z.C.
    An improved strategy for high-level production of TEV protease in Escherichia coli and its purification and characterization
    Protein Expr Purif51, 102-109. PubMed  Europe PubMed DOI  E
  75. Kraft,M., Radke,D., Wieland,G.D., Zipfel,P.F. and Horn,U.
    A fluorogenic substrate as quantitative in vivo reporter to determine protein expression and folding of tobacco etch virus protease in Escherichia coli
    Protein Expr Purif52, 478-484. PubMed  Europe PubMed DOI  A  E
    76. 2006
  76. Kawe,M., Forrer,P., Amstutz,P. and Pluckthun,A.
    Isolation of intracellular proteinase inhibitors derived from designed ankyrin repeat proteins by genetic screening
    J Biol Chem281, 40252-40263. PubMed  Europe PubMed DOI
  77. [YEAR:5-9-2006]van den Berg,S., Lofdahl,P.A., Hard,T. and Berglund,H.
    Improved solubility of TEV protease by directed evolution
    J Biotechnol121, 291-298. PubMed  Europe PubMed DOI
  78. [YEAR:29-10-2006]Wehr,M.C., Laage,R., Bolz,U., Fischer,T.M., Grunewald,S., Scheek,S., Bach,A., Nave,K.A. and Rossner,M.J.
    Monitoring regulated protein-protein interactions using split TEV
    Nat Methods3, 985-993. PubMed  Europe PubMed DOI
    79. 2005
  79. Adams,M.J., Antoniw,J.F. and Beaudoin,F.
    Overview and analysis of the polyprotein cleavage sites in the family Potyviridae
    Mol Plant Pathol6, 471-487. PubMed  Europe PubMed DOI  P
  80. [YEAR:1-7-2005]Nunn,C.M., Jeeves,M., Cliff,M.J., Urquhart,G.T., George,R.R., Chao,L.H., Tscuchia,Y. and Djordjevic,S.
    Crystal structure of tobacco etch virus protease shows the protein C terminus bound within the active site
    J Mol Biol350, 145-155. PubMed  Europe PubMed DOI  S
  81. Tozser,J., Tropea,J.E., Cherry,S., Bagossi,P., Copeland,T.D., Wlodawer,A. and Waugh,D.S.
    Comparison of the substrate specificity of two potyvirus proteases
    FEBS J272, 514-523. PubMed  Europe PubMed DOI  P
    82. 2004
  82. Han,J.S., Kim,D.H. and Choi,K.Y.
    Potyvirus NIa protease
    [ISSN:0-12-079610-4]2, 1376-1379.  V
    83. 2003
  83. Mohanty,A.K., Simmons,C.R. and Wiener,M.C.
    Inhibition of tobacco etch virus protease activity by detergents
    Protein Expr Purif27, 109-114. PubMed  Europe PubMed DOI
    84. 2002
  84. [YEAR:28-6-2002]Kapust,R.B., Tozser,J., Copeland,T.D. and Waugh,D.S.
    The P1' specificity of tobacco etch virus protease
    Biochem Biophys Res Commun294, 949-955. PubMed  Europe PubMed DOI  P
  85. [YEAR:27-12-2002]Phan,J., Zdanov,A., Evdokimov,A.G., Tropea,J.E., Peters,H.K., III, Kapust,R.B., Li,M., Wlodawer,A. and Waugh,D.S.
    Structural basis for the substrate specificity of tobacco etch virus protease
    J Biol Chem277, 50564-50572. PubMed  Europe PubMed DOI  P  S
    86. 2001
  86. [YEAR:28-9-2001]Faber,K.N., Kram,A.M., Ehrmann,M. and Veenhuis,M.
    A novel method to determine the topology of peroxisomal membrane proteins in vivo using the tobacco etch virus protease
    J Biol Chem276, 36501-36507. PubMed  Europe PubMed DOI
  87. Kapust,R.B., Tozser,J., Fox,J.D., Anderson,D.E., Cherry,S., Copeland,T.D. and Waugh,D.S.
    Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency
    Protein Eng14, 993-1000. PubMed  Europe PubMed
  88. Lucast,L.J., Batey,R.T. and Doudna,J.A.
    Large-scale purification of a stable form of recombinant tobacco etch virus protease
    Biotechniques30, 544-546. PubMed  Europe PubMed
    89. 2000
  89. Kapust,R.B. and Waugh,D.S.
    Controlled intracellular processing of fusion proteins by TEV protease
    Protein Expr Purif19, 312-318. PubMed  Europe PubMed DOI
  90. [YEAR:1-1-2000]Melcher,K.
    A modular set of prokaryotic and eukaryotic expression vectors
    Anal Biochem277, 109-120. PubMed  Europe PubMed DOI
  91. [YEAR:30-4-2000]Yoon,H.Y., Hwang,D.C., Choi,K.Y. and Song,B.D.
    Proteolytic processing of oligopeptides containing the target sequences by the recombinant tobacco vein mottling virus NIa proteinase
    Mol Cells10, 213-219. PubMed  Europe PubMed
    92. 1999
  92. Daros,J.A., Schaad,M.C. and Carrington,J.C.
    Functional analysis of the interaction between VPg-proteinase (NIa) and RNA polymerase (NIb) of tobacco etch potyvirus, using conditional and suppressor mutants
    J Virol73, 8732-8740. PubMed  Europe PubMed
    93. 1998
  93. Fellers,J.P., Collins,G.B. and Hunt,A.G.
    The NIa-proteinase of different plant potyviruses provides specific resistance to viral infection
    Crop Sci38, 1309-1319.
    94. 1997
  94. [YEAR:25-11-1997]Ehrmann,M., Bolek,P., Mondigler,M., Boyd,D. and Lange,R.
    TnTIN and TnTAP: mini-transposons for site-specific proteolysis in vivo
    Proc Natl Acad Sci U S A94, 13111-13115. PubMed  Europe PubMed DOI
  95. Li,X.H., Valdez,P., Olvera,R.E. and Carrington,J.C.
    Functions of the tobacco etch virus RNA polymerase (NIb): Subcellular transport and protein-protein interaction with VPg/proteinase (NIa)
    J Virol71, 1598-1607. PubMed  Europe PubMed
    96. 1996
  96. Schaad,M.C., Haldeman-Cahill,R., Cronin,S. and Carrington,J.C.
    Analysis of the VPg-proteinase (NIa) encoded by tobacco etch potyvirus: Effects of mutations on subcellular transport, proteolytic processing, and genome amplification
    J Virol70, 7039-7048. PubMed  Europe PubMed
    97. 1995
  97. Parks,T.D., Howard,E.D., Wolpert,T.J., Arp,D.J. and Dougherty,W.G.
    Expression and purification of a recombinant tobacco etch virus NIa proteinase: biochemical analyses of the full-length and a naturally occurring truncated proteinase form
    Virology210, 194-201. PubMed  Europe PubMed DOI
    98. 1992
  98. Parks,T.D., Smith,H.A. and Dougherty,W.G.
    Cleavage profiles of tobacco etch virus (TEV)-derived substrates mediated by precursor and processed forms of the TEV NIa proteinase
    J Gen Virol73, 149-155. PubMed  Europe PubMed
    99. 1991
  99. Parks,T.D. and Dougherty,W.G.
    Substrate recognition by the NIa proteinase of two potyviruses involves multiple domains: characterization using genetically engineered hybrid proteinase molecules
    Virology182, 17-27. PubMed  Europe PubMed DOI  P
    100. 1989
  100. Dougherty,W.G. and Parks,T.D.
    Molecular genetic and biochemical evidence for the involvement of the heptapeptide cleavage sequence in determining the reaction profile at two tobacco etch virus cleavage sites in cell-free assays
    Virology172, 145-155. PubMed  Europe PubMed
  101. Dougherty,W.G., Parks,T.D., Cary,S.M., Bazan,J.F. and Fletterick,R.J.
    Characterization of the catalytic residues of the tobacco etch virus 49-kDa proteinase
    Virology172, 302-310. PubMed  Europe PubMed DOI
    102. 1988
  102. Carrington,J.C. and Dougherty,W.G.
    A viral cleavage site cassette: identification of amino acid sequences required for tobacco etch virus polyprotein processing
    Proc Natl Acad Sci U S A85, 3391-3395. PubMed  Europe PubMed  P
  103. Carrington,J.C., Cary,S.M. and Dougherty,W.G.
    Mutational analysis of tobacco etch virus polyprotein processing: cis and trans proteolytic activities of polyproteins containing the 49-kilodalton proteinase
    J Virol62, 2313-2320. PubMed  Europe PubMed  P
    104. 1987
  104. Carrington,J.C. and Dougherty,W.G.
    Small nuclear inclusion protein encoded by a plant potyvirus genome is a protease
    J Virol61, 2540-2548. PubMed  Europe PubMed

feedback
Page created 8-September-2023

EMBL-EBI logo

© 2025 EBI
Terms of use