Literature for peptidase C04.004: tobacco etch virus NIa peptidase

Summary Alignment Sequences Sequence features Distribution Structure Literature Substrates

(Topics flags: S Structure, A Assay, P Specificity, L Localization, E Expression, U Therapeutic, V Review. To select only the references relevant to a single topic, click the link above. See explanation.)

2025

Bemelmans,M.P., Wetzel,B.N., Neusius,F.G., Tieves,F., Schwarz,C., Mateljak,I., Swiderek,K., Moliner,V., Alcalde,M. and Sieber,V. Engineering the Tobacco Etch Virus Protease toward a Platform for Traceless Cleavage Using Distal Site Prediction and Smart Library Design ACS Synth Biol (2025) PubMed Europe PubMed DOI
Davis,S.M., Romig,B.L., Abe,A.A. and Loening,N.M. An improved variant of tobacco etch virus (TEV) protease that does not need reducing agents Protein Sci (2025) 34, e70049-e70049. PubMed Europe PubMed DOI PMC EPMC
Gao,Z., Li,T., Ye,H., Ngai,W.S.C., Wang,H., Chen,P.R. and Wang,J. A gasdermin-based life-death evolution system for reprogramming protease specificity Nat Chem Biol (2025) PubMed Europe PubMed DOI
Mikolajczyk,B.M., Golinski,A.W. and Hackel,B.J. Enzyme-substrate co-display on yeast empowers engineering of tobacco etch virus protease activity Protein Eng Des Sel (2025) PubMed Europe PubMed DOI
Shams,R. and Regan,L. A New Highly Specific and Soluble Protease for Precise Removal of Nƒ_'Terminal Purification Tags ACS Omega (2025) 10, 30354-30364. PubMed Europe PubMed DOI PMC EPMC

2024

Beaumont,L.P., Mehalko,J., Johnson,A., Wall,V.E. and Esposito,D. Unexpected tobacco etch virus (TEV) protease cleavage of recombinant human proteins Protein Expr Purif (2024) 220, 106488-106488. PubMed Europe PubMed DOI
Cui,M., Lee,S., Ban,S.H., Ryu,J.R., Shen,M., Yang,S.H., Kim,J.Y., Choi,S.K., Han,J., Kim,Y., Han,K., Lee,D., Sun,W., Kwon,H.B. and Lee,D. A single-component, light-assisted uncaging switch for endoproteolytic release Nat Chem Biol (2024) 20, 353-364. PubMed Europe PubMed DOI
Devoy,C., Flores Bueso,Y., Buckley,S., Walker,S. and Tangney,M. Synthetic protein protease sensor platform Front Bioeng Biotechnol (2024) 12, 1347953-1347953. PubMed Europe PubMed DOI PMC EPMC
Golda,M., Hoffka,G., Cherry,S., Tropea,J.E., Lountos,G.T., Waugh,D.S., Wlodawer,A., Tozser,J. and Motyan,J.A. P1' specificity of the S219V/R203G mutant tobacco etch virus protease Proteins (2024) 92, 1085-1096-1096. PubMed Europe PubMed DOI
Lan,T., Peng,C., Yao,X., Chan,R.S.T., Wei,T., Rupanya,A., Radakovic,A., Wang,S., Chen,S., Lovell,S., Snyder,S.A., Bogyo,M. and Dickinson,B.C. Discovery of Thioether-Cyclized Macrocyclic Covalent Inhibitors by mRNA Display J Am Chem Soc (2024) 146, 24053-24060. PubMed Europe PubMed DOI
Martinusen,S.G., Slaton,E.W., Nelson,S.E., Pulgar,M.A., Besu,J.T., Simas,C.F. and Denard,C.A. Modular and integrative activity reporters enhance biochemical studies in the yeast ER Protein Eng Des Sel (2024) 37 PubMed Europe PubMed DOI PMC EPMC
Messing,S., Barnhart,K., Drew,M., Granato-Guerrero,N., Grose,C., Higgins,B., Hong,M., Hull,J., Perkins,S., Poon,I., Ramakrishnan,N., Seabolt,A., Taylor,T., Wall,V.E., Wright,N., Gillette,W. and Esposito,D. Improvements in large-scale production of Tobacco Etch Virus protease Protein Expr Purif (2024) , 106648-106648. PubMed Europe PubMed DOI
Shahriari,M., Shafiee,F., Moazen,F. and Mohammad Sadeghi,H.M. Optimization of the Production of Soluble Recombinant TEV Protease in Two E. coli Strains Avicenna J Med Biotechnol (2024) 16, 279-283. PubMed Europe PubMed DOI PMC EPMC
Sumida,K.H., Nunez-Franco,R., Kalvet,I., Pellock,S.J., Wicky,B.I.M., Milles,L.F., Dauparas,J., Wang,J., Kipnis,Y., Jameson,N., Kang,A., de la Cruz,J., Sankaran,B., Bera,A.K., Jimenez-Oses,G. and Baker,D. Improving Protein Expression, Stability, and Function with ProteinMPNN J Am Chem Soc (2024) 146, 2054-2061. PubMed Europe PubMed DOI PMC EPMC
Wang,J., Xu,Y., Wang,X., Li,J. and Hua,Z. Mechanism of Mutation-Induced Effects on the Catalytic Function of TEV Protease: A Molecular Dynamics Study Molecules (2024) 29 PubMed Europe PubMed DOI PMC EPMC
Wang,Y., Zheng,J., Wen,F., Tu,B. and Cui,L. Novel gnd_v2 Fusion Tag and Engineered TEV Protease Enable Efficient Production of Brazzein J Microbiol Biotechnol (2024) 34, 1-11. PubMed Europe PubMed DOI

2023

Fourest-Lieuvin,A., Vinit,A., Blot,B., Perrot,A., Denarier,E., Saudou,F. and Arnal,I. Controlled Tau Cleavage in Cells Reveals Abnormal Localizations of Tau Fragments Neuroscience (2023) 518, 162-177. PubMed Europe PubMed DOI
Meister,S.W., Parks,L., Kolmar,L., Mestre Borras,A., Stahl,S. and Lofblom,J. Engineering of TEV protease variants with redesigned substrate specificity Biotechnol J (2023) , e2200625-e2200625. PubMed Europe PubMed DOI
Parida,P.P., Saraswathi,D., Mopidevi,S.M.V. and Raran-Kurussi,S. Advancing large-scale production of TEV protease through an innovative NT* tag-based fusion construct Curr Res Struct Biol (2023) 6, 100106-100106. PubMed Europe PubMed DOI PMC EPMC
Winckler,L.I. and Dissmeyer,N. TEV protease cleavage in generation of artificial substrate proteins bearing neo-N-termini Methods Enzymol (2023) 686, 125-141. PubMed Europe PubMed DOI
Zheng,M. and Gao,J. Phage Display of Two Distinct Warheads to Inhibit Challenging Proteins ACS Chem Biol (2023) 18, 2259-2266. PubMed Europe PubMed DOI

2022

Enriquez-Flores,S., De la Mora-De la Mora, Flores-Lopez,L.A., Cabrera,N., Fernandez-Lainez,C., Hernandez-Alcantara,G., Guerrero-Beltran,C.E., Lopez-Velazquez,G. and Garcia-Torres,I. Improved yield, stability, and cleavage reaction of a novel tobacco etch virus protease mutant Appl Microbiol Biotechnol (2022) 106, 1475-1492. PubMed Europe PubMed DOI V
Paththamperuma,C. and Page,R.C. Fluorescence dequenching assay for the activity of TEV protease Anal Biochem (2022) 659, 114954-114954. PubMed Europe PubMed DOI PMC EPMC
Renna,P., Ripoli,C., Dagliyan,O., Pastore,F., Rinaudo,M., Re,A., Paciello,F. and Grassi,C. Engineering a switchable single-chain TEV protease to control protein maturation in living neurons Bioeng Transl Med (2022) 7, e10292-e10292. PubMed Europe PubMed DOI PMC EPMC
Zlobin,A. and Golovin,A. Between Protein Fold and Nucleophile Identity: Multiscale Modeling of the TEV Protease Enzyme-Substrate Complex ACS Omega (2022) 7, 40279-40292. PubMed Europe PubMed DOI PMC EPMC

2021

Alonso-Gardon,M. and Estevez,R. Split-Tobacco Etch Virus (Split-TEV) Method in G Protein-Coupled Receptor Interacting Proteins Methods Mol Biol (2021) 2268, 223-232. PubMed Europe PubMed DOI
Bayar,E., Ren,Y., Chen,Y., Hu,Y., Zhang,S., Yu,X. and Fan,J. Construction, investigation and application of the TEV protease variants with the improved oxidative stability J Microbiol Biotechnol (2021) 31 PubMed Europe PubMed DOI
Denard,C.A., Paresi,C., Yaghi,R., McGinnis,N., Bennett,Z., Yi,L., Georgiou,G. and Iverson,B.L. YESS 2.0, a Tunable Platform for Enzyme Evolution, Yields Highly Active TEV Protease Variants ACS Synth Biol (2021) 10, 63-71. PubMed Europe PubMed DOI

2020

Brungardt,J., Govind,R. and Trick,H.N. A simplified method for producing laboratory grade recombinant TEV protease from E. coli Protein Expr Purif (2020) 174, 105662-105662. PubMed Europe PubMed DOI E
Chen,S., Lovell,S., Lee,S., Fellner,M., Mace,P.D. and Bogyo,M. Identification of highly selective covalent inhibitors by phage display Nat Biotechnol (2020) PubMed Europe PubMed DOI
Chung,H.K. and Lin,M.Z. On the cutting edge: protease-based methods for sensing and controlling cell biology Nat Methods (2020) PubMed Europe PubMed DOI V
Cupo,R.R. and Shorter,J. Expression and Purification of Recombinant Skd3 (Human ClpB) Protein and Tobacco Etch Virus (TEV) Protease from Escherichia coli Bio Protoc (2020) 10, e3858-e3858. PubMed Europe PubMed DOI PMC EPMC
Deng,Y., Shi,S., Zheng,B., Wu,T. and Zheng,P. Enzymatic Construction of Protein Polymer/Polyprotein Using OaAEP1 and TEV Protease Bio Protoc (2020) 10, e3596-e3596. PubMed Europe PubMed DOI PMC EPMC
Holt,B.A. and Kwong,G.A. Protease circuits for processing biological information Nat Commun (2020) 11, 5021-5021. PubMed Europe PubMed DOI PMC EPMC
Lakshmanan,A., Jin,Z., Nety,S.P., Sawyer,D.P., Lee-Gosselin,A., Malounda,D., Swift,M.B., Maresca,D. and Shapiro,M.G. Acoustic biosensors for ultrasound imaging of enzyme activity Nat Chem Biol (2020) PubMed Europe PubMed DOI
Mohammadian,N., Mohammadian,H., Moazen,F., Pakdel,M.H., Jahanian-Najafabadi,A. and Mir Mohammad Sadeghi,H. Optimization of solvent media to solubilize TEV protease using response surface method Res Pharm Sci (2020) 15, 331-339. PubMed Europe PubMed DOI PMC EPMC
Nam,H., Hwang,B.J., Choi,D.Y., Shin,S. and Choi,M. Tobacco etch virus (TEV) protease with multiple mutations to improve solubility and reduce self-cleavage exhibits enhanced enzymatic activity FEBS Open Bio (2020) 10, 619-626. PubMed Europe PubMed DOI E
Norris,J.L., Patel,T., Dasari,A.K.R., Cope,T.A., Lim,K.H. and Hughes,R.M. Covalent and non-covalent strategies for the immobilization of Tobacco Etch Virus protease (TEVp) on superparamagnetic nanoparticles J Biotechnol (2020) 322, 1-9. PubMed Europe PubMed DOI
Sanchez,M.I. and Ting,A.Y. Directed evolution improves the catalytic efficiency of TEV protease Nat Methods (2020) 17, 167-174. PubMed Europe PubMed DOI L
Sanchez,M.I., Nguyen,Q.A., Wang,W., Soltesz,I. and Ting,A.Y. Transcriptional readout of neuronal activity via an engineered Ca(2+)-activated protease Proc Natl Acad Sci U S A (2020) 117, 33186-33196. PubMed Europe PubMed DOI

2018

Nautiyal,K. and Kuroda,Y. A SEP tag enhances the expression, solubility and yield of recombinant TEV protease without altering its activity N Biotechnol (2018) 42, 77-84. PubMed Europe PubMed DOI E
O'Neill,B.K. and Laughlin,S.T. Neuronal calcium recording with an engineered TEV protease ACS Chem Biol (2018) 13, 1159-1164. PubMed Europe PubMed DOI

2017

Chen,Y., Li,Q., Yang,J. and Xie,H. Promoting tag removal of a MBP-fused integral membrane protein by TEV protease Appl Biochem Biotechnol (2017) 181, 939-947. PubMed Europe PubMed DOI
Packer,M.S., Rees,H.A. and Liu,D.R. Phage-assisted continuous evolution of proteases with altered substrate specificity Nat Commun (2017) 8, 956-956. PubMed Europe PubMed DOI PMC EPMC
Raran-Kurussi,S., Cherry,S., Zhang,D. and Waugh,D.S. Removal of affinity tags with TEV protease Methods Mol Biol (2017) 1586, 221-230. PubMed Europe PubMed DOI
Ren,S., Zhu,G. and Du,L. Study on the impacts of Co2+ on the tobacco etch virus protease Chin J App Eviron Biol (2017) 23, 113-116. DOI

2016

Cesaratto,F., Burrone,O.R. and Petris,G. Tobacco etch virus protease: a shortcut across biotechnologies J Biotechnol (2016) 231, 239-249. PubMed Europe PubMed DOI
He,Y.H., Li,Y.M. and Chen,Y.X. Phosphorylation regulates proteolytic efficiency of TEV protease detected by a 5(6)-carboxyfluorescein-pyrene based fluorescent sensor Talanta (2016) 150, 340-345. PubMed Europe PubMed DOI

2015

Cesaratto,F., Lopez-Requena,A., Burrone,O.R. and Petris,G. Engineered tobacco etch virus (TEV) protease active in the secretory pathway of mammalian cells J Biotechnol (2015) 212, 159-166. PubMed Europe PubMed DOI
Gerhardt,K. and Tabor,J. Optogenetic regulation of proteolysis using an engineered light-switchable split Tev protease Synth Biol : Eng , Evol , Des Conf , SEED (2015) 2, 804-805.
Shafee,T., Gatti-Lafranconi,P., Minter,R. and Hollfelder,F. Handicap-recover evolution leads to a chemically versatile, nucleophile-permissive protease Chembiochem (2015) 16, 1866-1869. PubMed Europe PubMed DOI
Zhu,G.-F., Ren,S.-Y., Xi,L., Du,L.-F. and Zhu,X.-F. Temperature induced structural transitions from native to unfolded aggregated states of tobacco etch virus protease J Mol Struct (2015) 1082, 80-90. DOI

2014

Fang,J., Zou,L., Zhou,X., Cheng,B. and Fan,J. Synonymous rare arginine codons and tRNA abundance affect protein production and quality of TEV protease variant PLoS ONE (2014) 9, e112254-e112254. PubMed Europe PubMed DOI E

2013

Fang,J., Chen,L., Cheng,B. and Fan,J. Engineering soluble tobacco etch virus protease accompanies the loss of stability Protein Expr Purif (2013) 92, 29-35. PubMed Europe PubMed DOI
Raran-Kurussi,S., Tozser,J., Cherry,S., Tropea,J.E. and Waugh,D.S. Differential temperature dependence of tobacco etch virus and rhinovirus 3C proteases Anal Biochem (2013) 436, 142-144. PubMed Europe PubMed DOI
Renicke,C., Spadaccini,R. and Taxis,C. A tobacco etch virus protease with increased substrate tolerance at the P1' position PLoS ONE (2013) 8, e67915-e67915. PubMed Europe PubMed DOI
Wang,J.-Z., Ren,S.-Y., Zhu,G.-F., Xi,L., Han,Y.-G., Luo,Y. and Du,L.-F. Hg2+ interference with the structure of tobacco etch virus protease (TEVp) and its implications for biological engineering J Mol Struct (2013) 1052, 85-92. DOI
Wang,Y., Zhu,G.F., Ren,S.Y., Han,Y.G., Luo,Y. and Du,L.F. Insight into the structural stability of wild type and mutants of the tobacco etch virus protease with molecular dynamics simulations J Mol Model (2013) 19, 4865-4875. PubMed Europe PubMed DOI
Yi,L., Gebhard,M.C., Li,Q., Taft,J.M., Georgiou,G. and Iverson,B.L. Engineering of TEV protease variants by yeast ER sequestration screening (YESS) of combinatorial libraries Proc Natl Acad Sci U S A (2013) 110, 7229-7234. PubMed Europe PubMed DOI

2012

Han,J.S., Kim,D.H. and Choi,K.Y. Potyvirus NIa protease [ISSN:978-0-12-407743-0] (2012) 3, 2427-2432. DOI
Kim,T.K., Han,H.E., Kim,H., Lee,J.E., Choi,D., Park,W.J. and Han,P.L. Expression of the plant viral protease NIa in the brain of a mouse model of Alzheimer's disease mitigates Abeta pathology and improves cognitive function Exp Mol Med (2012) 44, 740-748. PubMed Europe PubMed DOI U
Miladi,B., Marjou,A.E., Boeuf,G., Bouallagui,H., Dufour,F., Di Martino,P. and Elm'selmi,A. Oriented immobilization of the tobacco etch virus protease for the cleavage of fusion proteins J Biotechnol (2012) 158, 97-103. PubMed Europe PubMed DOI
Sun,C., Liang,J., Shi,R., Gao,X., Zhang,R., Hong,F., Yuan,Q. and Wang,S. Tobacco etch virus protease retains its activity in various buffers and in the presence of diverse additives Protein Expr Purif (2012) 82, 226-231. PubMed Europe PubMed DOI
Verhoeven,K.D., Altstadt,O.C. and Savinov,S.N. Intracellular detection and evolution of site-specific proteases using a genetic selection system Appl Biochem Biotechnol (2012) 166, 1340-1354. PubMed Europe PubMed DOI
Wei,L., Cai,X., Qi,Z., Rong,L., Cheng,B. and Fan,J. In vivo and in vitro characterization of TEV protease mutants Protein Expr Purif (2012) 83, 157-163. PubMed Europe PubMed DOI E

2011

Djannatian,M.S., Galinski,S., Fischer,T.M. and Rossner,M.J. Studying G protein-coupled receptor activation using split-tobacco etch virus assays Anal Biochem (2011) 412, 141-152. PubMed Europe PubMed DOI
Kostallas,G., Lofdahl,P.A. and Samuelson,P. Substrate profiling of tobacco etch virus protease using a novel fluorescence-assisted whole-cell assay PLoS ONE (2011) 6, e16136-e16136. PubMed Europe PubMed DOI PMC EPMC P
Miladi,B., Bouallagui,H., Dridi,C., El Marjou,A., Boeuf,G., Di Martino,P., Dufour,F. and Elm'selmi,A. A new tagged-TEV protease: construction, optimisation of production, purification and test activity Protein Expr Purif (2011) 75, 75-82. PubMed Europe PubMed DOI

2010

Boulware,K.T., Jabaiah,A. and Daugherty,P.S. Evolutionary optimization of peptide substrates for proteases that exhibit rapid hydrolysis kinetics Biotechnol Bioeng (2010) 106, 339-346. PubMed Europe PubMed DOI P

2009

Puhl,A.C., Giacomini,C., Irazoqui,G., Batista-Viera,F., Villarino,A. and Terenzi,H. Covalent immobilization of tobacco-etch-virus NIa protease: a useful tool for cleavage of the histidine tag of recombinant proteins Biotechnol Appl Biochem (2009) 53, 165-174. PubMed Europe PubMed DOI
Wu,X., Wu,D., Lu,Z., Chen,W., Hu,X. and Ding,Y. A novel method for high-level production of TEV protease by superfolder GFP tag J Biomed Biotechnol (2009) 2009, 591923-591923. PubMed Europe PubMed DOI PMC EPMC E

2008

Harder,B., Schomburg,A., Pflanz,R., Kustner,K., Gerlach,N. and Schuh,R. TEV protease-mediated cleavage in Drosophila as a tool to analyze protein functions in living organisms Biotechniques (2008) 44, 765-772. PubMed Europe PubMed DOI
Lundback,A.K., van den Berg,S., Hebert,H., Berglund,H. and Eshaghi,S. Exploring the activity of tobacco etch virus protease in detergent solutions Anal Biochem (2008) 382, 69-71. PubMed Europe PubMed DOI
Pauli,A., Althoff,F., Oliveira,R.A., Heidmann,S., Schuldiner,O., Lehner,C.F., Dickson,B.J. and Nasmyth,K. Cell-type-specific TEV protease cleavage reveals cohesin functions in Drosophila neurons Dev Cell (2008) 14, 239-251. PubMed Europe PubMed DOI PMC EPMC
Wehr,M.C., Reinecke,L., Botvinnik,A. and Rossner,M.J. Analysis of transient phosphorylation-dependent protein-protein interactions in living mammalian cells using split-TEV BMC Biotechnol (2008) 8, 55-55. PubMed Europe PubMed DOI PMC EPMC

2007

Blommel,P.G. and Fox,B.G. A combined approach to improving large-scale production of tobacco etch virus protease Protein Expr Purif (2007) 55, 53-68. PubMed Europe PubMed DOI PMC EPMC E
Cabrita,L.D., Gilis,D., Robertson,A.L., Dehouck,Y., Rooman,M. and Bottomley,S.P. Enhancing the stability and solubility of TEV protease using in silico design Protein Sci (2007) 16, 2360-2367. PubMed Europe PubMed DOI PMC EPMC
Eser,M., Henrichs,T., Boyd,D. and Ehrmann,M. Target-directed proteolysis in vivo Methods Enzymol (2007) 421, 68-83. PubMed Europe PubMed DOI
Fang,L., Jia,K.Z., Tang,Y.L., Ma,D.Y., Yu,M. and Hua,Z.C. An improved strategy for high-level production of TEV protease in Escherichia coli and its purification and characterization Protein Expr Purif (2007) 51, 102-109. PubMed Europe PubMed DOI E
Kraft,M., Radke,D., Wieland,G.D., Zipfel,P.F. and Horn,U. A fluorogenic substrate as quantitative in vivo reporter to determine protein expression and folding of tobacco etch virus protease in Escherichia coli Protein Expr Purif (2007) 52, 478-484. PubMed Europe PubMed DOI A E

2006

Kawe,M., Forrer,P., Amstutz,P. and Pluckthun,A. Isolation of intracellular proteinase inhibitors derived from designed ankyrin repeat proteins by genetic screening J Biol Chem (2006) 281, 40252-40263. PubMed Europe PubMed DOI
[YEAR:5-9-2006]van den Berg,S., Lofdahl,P.A., Hard,T. and Berglund,H. Improved solubility of TEV protease by directed evolution J Biotechnol (5-9-2006) 121, 291-298. PubMed Europe PubMed DOI
[YEAR:29-10-2006]Wehr,M.C., Laage,R., Bolz,U., Fischer,T.M., Grunewald,S., Scheek,S., Bach,A., Nave,K.A. and Rossner,M.J. Monitoring regulated protein-protein interactions using split TEV Nat Methods (29-10-2006) 3, 985-993. PubMed Europe PubMed DOI

2005

Adams,M.J., Antoniw,J.F. and Beaudoin,F. Overview and analysis of the polyprotein cleavage sites in the family Potyviridae Mol Plant Pathol (2005) 6, 471-487. PubMed Europe PubMed DOI P
[YEAR:1-7-2005]Nunn,C.M., Jeeves,M., Cliff,M.J., Urquhart,G.T., George,R.R., Chao,L.H., Tscuchia,Y. and Djordjevic,S. Crystal structure of tobacco etch virus protease shows the protein C terminus bound within the active site J Mol Biol (1-7-2005) 350, 145-155. PubMed Europe PubMed DOI S
Tozser,J., Tropea,J.E., Cherry,S., Bagossi,P., Copeland,T.D., Wlodawer,A. and Waugh,D.S. Comparison of the substrate specificity of two potyvirus proteases FEBS J (2005) 272, 514-523. PubMed Europe PubMed DOI P

2004

Han,J.S., Kim,D.H. and Choi,K.Y. Potyvirus NIa protease [ISSN:0-12-079610-4] (2004) 2, 1376-1379. V

2003

Mohanty,A.K., Simmons,C.R. and Wiener,M.C. Inhibition of tobacco etch virus protease activity by detergents Protein Expr Purif (2003) 27, 109-114. PubMed Europe PubMed DOI

2002

[YEAR:28-6-2002]Kapust,R.B., Tozser,J., Copeland,T.D. and Waugh,D.S. The P1' specificity of tobacco etch virus protease Biochem Biophys Res Commun (28-6-2002) 294, 949-955. PubMed Europe PubMed DOI P
[YEAR:27-12-2002]Phan,J., Zdanov,A., Evdokimov,A.G., Tropea,J.E., Peters,H.K., III, Kapust,R.B., Li,M., Wlodawer,A. and Waugh,D.S. Structural basis for the substrate specificity of tobacco etch virus protease J Biol Chem (27-12-2002) 277, 50564-50572. PubMed Europe PubMed DOI P S

2001

[YEAR:28-9-2001]Faber,K.N., Kram,A.M., Ehrmann,M. and Veenhuis,M. A novel method to determine the topology of peroxisomal membrane proteins in vivo using the tobacco etch virus protease J Biol Chem (28-9-2001) 276, 36501-36507. PubMed Europe PubMed DOI
Kapust,R.B., Tozser,J., Fox,J.D., Anderson,D.E., Cherry,S., Copeland,T.D. and Waugh,D.S. Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency Protein Eng (2001) 14, 993-1000. PubMed Europe PubMed
Lucast,L.J., Batey,R.T. and Doudna,J.A. Large-scale purification of a stable form of recombinant tobacco etch virus protease Biotechniques (2001) 30, 544-546. PubMed Europe PubMed

2000

Kapust,R.B. and Waugh,D.S. Controlled intracellular processing of fusion proteins by TEV protease Protein Expr Purif (2000) 19, 312-318. PubMed Europe PubMed DOI
[YEAR:1-1-2000]Melcher,K. A modular set of prokaryotic and eukaryotic expression vectors Anal Biochem (1-1-2000) 277, 109-120. PubMed Europe PubMed DOI
[YEAR:30-4-2000]Yoon,H.Y., Hwang,D.C., Choi,K.Y. and Song,B.D. Proteolytic processing of oligopeptides containing the target sequences by the recombinant tobacco vein mottling virus NIa proteinase Mol Cells (30-4-2000) 10, 213-219. PubMed Europe PubMed

1999

Daros,J.A., Schaad,M.C. and Carrington,J.C. Functional analysis of the interaction between VPg-proteinase (NIa) and RNA polymerase (NIb) of tobacco etch potyvirus, using conditional and suppressor mutants J Virol (1999) 73, 8732-8740. PubMed Europe PubMed PMC EPMC

1998

Fellers,J.P., Collins,G.B. and Hunt,A.G. The NIa-proteinase of different plant potyviruses provides specific resistance to viral infection Crop Sci (1998) 38, 1309-1319.

1997

[YEAR:25-11-1997]Ehrmann,M., Bolek,P., Mondigler,M., Boyd,D. and Lange,R. TnTIN and TnTAP: mini-transposons for site-specific proteolysis in vivo Proc Natl Acad Sci U S A (25-11-1997) 94, 13111-13115. PubMed Europe PubMed DOI PMC EPMC
Li,X.H., Valdez,P., Olvera,R.E. and Carrington,J.C. Functions of the tobacco etch virus RNA polymerase (NIb): Subcellular transport and protein-protein interaction with VPg/proteinase (NIa) J Virol (1997) 71, 1598-1607. PubMed Europe PubMed PMC EPMC

1996

Schaad,M.C., Haldeman-Cahill,R., Cronin,S. and Carrington,J.C. Analysis of the VPg-proteinase (NIa) encoded by tobacco etch potyvirus: Effects of mutations on subcellular transport, proteolytic processing, and genome amplification J Virol (1996) 70, 7039-7048. PubMed Europe PubMed PMC EPMC

1995

Parks,T.D., Howard,E.D., Wolpert,T.J., Arp,D.J. and Dougherty,W.G. Expression and purification of a recombinant tobacco etch virus NIa proteinase: biochemical analyses of the full-length and a naturally occurring truncated proteinase form Virology (1995) 210, 194-201. PubMed Europe PubMed DOI

1992

Parks,T.D., Smith,H.A. and Dougherty,W.G. Cleavage profiles of tobacco etch virus (TEV)-derived substrates mediated by precursor and processed forms of the TEV NIa proteinase J Gen Virol (1992) 73, 149-155. PubMed Europe PubMed

1991

Parks,T.D. and Dougherty,W.G. Substrate recognition by the NIa proteinase of two potyviruses involves multiple domains: characterization using genetically engineered hybrid proteinase molecules Virology (1991) 182, 17-27. PubMed Europe PubMed DOI P

1989

Dougherty,W.G., Parks,T.D., Cary,S.M., Bazan,J.F. and Fletterick,R.J. Characterization of the catalytic residues of the tobacco etch virus 49-kDa proteinase Virology (1989) 172, 302-310. PubMed Europe PubMed DOI
Dougherty,W.G. and Parks,T.D. Molecular genetic and biochemical evidence for the involvement of the heptapeptide cleavage sequence in determining the reaction profile at two tobacco etch virus cleavage sites in cell-free assays Virology (1989) 172, 145-155. PubMed Europe PubMed

1988

Carrington,J.C., Cary,S.M. and Dougherty,W.G. Mutational analysis of tobacco etch virus polyprotein processing: cis and trans proteolytic activities of polyproteins containing the 49-kilodalton proteinase J Virol (1988) 62, 2313-2320. PubMed Europe PubMed PMC EPMC P
Carrington,J.C. and Dougherty,W.G. A viral cleavage site cassette: identification of amino acid sequences required for tobacco etch virus polyprotein processing Proc Natl Acad Sci U S A (1988) 85, 3391-3395. PubMed Europe PubMed PMC EPMC P

1987

Carrington,J.C. and Dougherty,W.G. Small nuclear inclusion protein encoded by a plant potyvirus genome is a protease J Virol (1987) 61, 2540-2548. PubMed Europe PubMed PMC EPMC