Literature for peptidase C01.005: stem bromelain

Summary Alignment Sequences Sequence features Distribution Structure Literature Substrates

(Topics flags: A Assay, S Structure, P Specificity, I Inhibitor, E Expression, U Therapeutic, V Review. To select only the references relevant to a single topic, click the link above. See explanation.)

2025

Kumar,V., Mangla,B., Javed,S., Ahsan,W., Kumar,P., Garg,V. and Dureja,H. Correction: Bromelain: a review of its mechanisms, pharmacological effects and potential applications Food Funct (2025) PubMed Europe PubMed DOI
Lugo,M.C., Kammura,A., Kobayashi,T., Ito,M., Harada,T. and Umemura,K. Amyloidogenic Growth Observation of Stem Bromelain via Atomic Force Microscopy ACS Omega (2025) 10, 49002-49009. PubMed Europe PubMed DOI PMC EPMC

2024

Chiarelli,P.G., Martinez,B., Nakamura,T. and Mis Solval,K. Enhancing Bromelain Recovery from Pineapple By-Products: A Sustainable Approach for Value Addition and Waste Reduction Foods (2024) 13 PubMed Europe PubMed DOI PMC EPMC
Kansakar,U., Trimarco,V., Manzi,M.V., Cervi,E., Mone,P. and Santulli,G. Exploring the Therapeutic Potential of Bromelain: Applications, Benefits, and Mechanisms Nutrients (2024) 16 PubMed Europe PubMed DOI PMC EPMC V
Kaushal,R., Kaur,B., Panesar,P.S., Anal,A.K. and Chu-Ky,S. Valorization of pineapple rind for bromelain extraction using microwave assisted technique: optimization, purification, and structural characterization J Food Sci Technol (2024) 61, 551-562. PubMed Europe PubMed DOI PMC EPMC
Khan,T., Halder,B., Das,N. and Sen,P. Role of Associated Water Dynamics on Protein Stability and Activity in Crowded Milieu J Phys Chem B (2024) 128, 8672-8686. PubMed Europe PubMed DOI
Locci,C., Chicconi,E. and Antonucci,R. Current Uses of Bromelain in Children: A Narrative Review Children (Basel) (2024) 11 PubMed Europe PubMed DOI PMC EPMC V
Pillai,K., Akhter,J., Mekkawy,A.H., Valle,S.J. and Morris,D.L. Development and Validation of Micro-Azocasein Assay for Quantifying Bromelain Methods Protoc (2024) 7 PubMed Europe PubMed DOI PMC EPMC
Porfirio,M.C.P., Santos,J.B., Alves,A.N., Santos,L.S., Bonomo,R.C.F. and da Costa Ilheu Fontan,R. Purification of pineapple bromelain by IMAC chromatography using chlorophyll-activated macroporous matrices J Chromatogr B Analyt Technol Biomed Life Sci (2024) 1234, 124027-124027. PubMed Europe PubMed DOI
Wajih,N., Erali,R.A., Forsythe,S.D., Schaaf,C.R., Shen,P., Levine,E.A., Soker,S., Morris,D.L. and Votanopoulos,K.I. Enhancing the Efficacy of HIPEC Through Bromelain: A Preclinical Investigation in Appendiceal Cancer Ann Surg Oncol (2024) 31, 5377-5389. PubMed Europe PubMed DOI

2023

Kumar,V., Mangla,B., Javed,S., Ahsan,W., Kumar,P., Garg,V. and Dureja,H. Bromelain: a review of its mechanisms, pharmacological effects and potential applications Food Funct (2023) 14, 8101-8128. PubMed Europe PubMed DOI
Leelakanok,N., Petchsomrit,A., Janurai,T., Saechan,C. and Sunsandee,N. Efficacy and safety of bromelain: A systematic review and meta-analysis Nutr Health (2023) , 2601060231173732-2601060231173732. PubMed Europe PubMed DOI
Liu,X., Huang,Q., Pan,P., Fang,M., Zhang,Y., Yang,S., Li,M. and Liu,Y. Comparative Study of the Preparation of High-Molecular-Weight Fibroin by Degumming Silk with Several Neutral Proteases Polymers (Basel) (2023) 15 PubMed Europe PubMed DOI PMC EPMC
Sharma,G. and Vimal,A. Bromelain: An Enzyme Expanding its Horizon from Food to Pharmaceutical Industry Curr Pharm Biotechnol (2023) 24, 1715-1726. PubMed Europe PubMed DOI V
Wen,H.K., Valle,S.J. and Morris,D.L. Bromelain and acetylcysteine (BromAc((R))): a novel approach to the treatment of mucinous tumours Am J Cancer Res (2023) 13, 1522-1532. PubMed Europe PubMed PMC EPMC V

2022

Agrawal,P., Nikhade,P., Patel,A., Mankar,N. and Sedani,S. Bromelain: A Potent Phytomedicine Cureus (2022) 14, e27876-e27876. PubMed Europe PubMed DOI PMC EPMC V
Fathi,A.N., Babaei,S., Babaei,S., Baazm,M., Sakhaie,H. and Babaei,S. Effect of bromelain on mast cell numbers and degranulation in diabetic rat wound healing J Wound Care (2022) 31, S4-S4. PubMed Europe PubMed DOI
Gamarra,F.M.C., Santana,J.C.C., Llanos,S.A.V., Perez,J.A.H., Flausino,F.R., Quispe,A.P.B., Mendoza,P.C., Vanalle,R.M., Carreno-Farfan,C., Berssaneti,F.T., de Souza,R.R. and Tambourgi,E.B. High Retention and Purification of Bromelain Enzyme (Ananas comosus L. Merrill) from Pineapple Juice Using Plain and Hollow Polymeric Membranes Techniques Polymers (Basel) (2022) 14 PubMed Europe PubMed DOI PMC EPMC
Pezzani,R., Jimenez-Garcia,M., Capo,X., Sonmez Gurer,E., Sharopov,F., Rachel,T.Y.L., Ntieche Woutouoba,D., Rescigno,A., Peddio,S., Zucca,P., Tsouh Fokou,P.V., Martorell,M., Gulsunoglu-Konuskan,Z., Ydyrys,A., Bekzat,T., Gulmira,T., Hano,C., Sharifi-Rad,J. and Calina,D. Anticancer properties of bromelain: State-of-the-art and recent trends Front Oncol (2022) 12, 1068778-1068778. PubMed Europe PubMed DOI PMC EPMC V

2021

Arkoulis,N., Mabvuure,N.T., Smith,A. and Barnes,D.E. Early experiences using bromelain-based enzymatic debridement in a tertiary burns centre in the United Kingdom: A retrospective case series review J Plast Reconstr Aesthet Surg (2021) 74, 1402-1407. PubMed Europe PubMed DOI
Ataide,J.A., Cefali,L.C., Figueiredo,M.C., Braga,L.E.O., Ruiz,A.L.T.G., Foglio,M.A., Oliveira-Nascimento,L. and Mazzola,P.G. In vitro performance of free and encapsulated bromelain Sci Rep (2021) 11, 10195-10195. PubMed Europe PubMed DOI PMC EPMC
Hikisz,P. and Bernasinska-Slomczewska,J. Beneficial Properties of Bromelain Nutrients (2021) 13 PubMed Europe PubMed DOI PMC EPMC V
Varilla,C., Marcone,M., Paiva,L. and Baptista,J. Bromelain, a Group of Pineapple Proteolytic Complex Enzymes (Ananas comosus) and Their Possible Therapeutic and Clinical Effects. A Summary Foods (2021) 10 PubMed Europe PubMed DOI PMC EPMC
Zhou,W., Ye,C., Geng,L., Chen,G., Wang,X., Chen,W., Sa,R., Zhang,J. and Zhang,X. Purification and characterization of bromelain from pineapple (Ananas comosus L.) peel waste J Food Sci (2021) 86, 385-393. PubMed Europe PubMed DOI

2020

Azarkan,M., Maquoi,E., Delbrassine,F., Herman,R., M'rabet,N., Calvo Esposito,R., Charlier,P. and Kerff,F. Structures of the free and inhibitors-bound forms of bromelain and ananain from Ananas comosus stem and in vitro study of their cytotoxicity Sci Rep (2020) 10, 19570-19570. PubMed Europe PubMed DOI PMC EPMC I
Bakare,A.O. and Owoyele,B.V. Antinociceptive and neuroprotective effects of bromelain in chronic constriction injury-induced neuropathic pain in Wistar rats Korean J Pain (2020) 33, 13-22. PubMed Europe PubMed DOI
Bakare,A.O. and Owoyele,B.V. Bromelain reversed electrolyte imbalance in the chronically constricted sciatic nerve of Wistar rats Naunyn Schmiedebergs Arch Pharmacol (2020) 393, 457-467. PubMed Europe PubMed DOI U
Gross,P., Seelert,H., Meiser,P. and Muller,R. Characterization of bromelain indicates a molar excess of inhibitor vs. enzyme molecules, a Jacalin-like lectin and Maillard reaction products J Pharm Biomed Anal (2020) 181, 113075-113075. PubMed Europe PubMed DOI
Harats,M., Haik,J., Cleary,M., Vashurin,I., Aviv,U. and Kornhaber,R. A retrospective review of an off-label bromelain-based selective enzymatic debridement (Nexobrid(R)) in the treatment of deep, partial, and full thickness burns and hard to heal wounds Isr Med Assoc J (2020) 22, 83-88. PubMed Europe PubMed U
Jebur,A.B., El-Demerdash,F.M. and Kang,W. Bromelain from Ananas comosus stem attenuates oxidative toxicity and testicular dysfunction caused by aluminum in rats J Trace Elem Med Biol (2020) 62, 126631-126631. PubMed Europe PubMed DOI
Kritis,P., Karampela,I., Kokoris,S. and Dalamaga,M. The combination of bromelain and curcumin as an immune-boosting nutraceutical in the prevention of severe COVID-19 Metabol Open (2020) 8, 100066-100066. PubMed Europe PubMed DOI PMC EPMC I
Mekkawy,M.H., Fahmy,H.A., Nada,A.S. and Ali,O.S. Study of the Radiosensitizing and Radioprotective Efficacy of Bromelain (a Pineapple Extract): In Vitro and In Vivo Integr Cancer Ther (2020) 19, 1534735420950468-1534735420950468. PubMed Europe PubMed DOI PMC EPMC
Mogha,N.K., Sindhu,A. and Venkatesu,P. A biophysical strategy to examine the impact of newly synthesized polymerizable ammonium-based ionic liquids on the structural stability and proteolytic activity of stem bromelain Int J Biol Macromol (2020) 151, 957-966. PubMed Europe PubMed DOI

2019

De Souza,G.M., Fernandes,I.A., Dos Santos,C.R.R. and Falci,S.G.M. Is bromelain effective in controlling the inflammatory parameters of pain, edema, and trismus after lower third molar surgery? A systematic review and meta-analysis Phytother Res (2019) 33, 473-481. PubMed Europe PubMed DOI U
Ghensi,P., Cucchi,A., Bonaccorso,A., Ferroni,L., Gardin,C., Mortellaro,C. and Zavan,B. In vitro effect of bromelain on the regenerative properties of mesenchymal stem cells J Craniofac Surg (2019) 30, 1064-1067. PubMed Europe PubMed DOI U
Han,J., Wang,L., Wang,L., Li,C., Mao,Y. and Wang,Y. Fabrication of a core-shell-shell magnetic polymeric microsphere with excellent performance for separation and purification of bromelain Food Chem (2019) 283, 1-10. PubMed Europe PubMed DOI
Han,J., Cai,Y., Wang,L., Mao,Y., Ni,L. and Wang,Y. A high efficiency method combining metal chelate ionic liquid-based aqueous two-phase flotation with two-step precipitation process for bromelain purification Food Chem (2019) , 125749-125749. PubMed Europe PubMed DOI
Lee,J.H., Lee,J.T., Park,H.R. and Kim,J.B. The potential use of bromelain as a natural oral medicine having anticarcinogenic activities Food Sci Nutr (2019) 7, 1656-1667. PubMed Europe PubMed DOI PMC EPMC U
Luo,P., Han,J., Li,Y., Wang,Y., Wang,L. and Ni,L. Preparation of dendritic polymer-based magnetic carrier for application of bromelain separation and purification J Food Biochem (2019) 43, e12976-e12976. PubMed Europe PubMed DOI
Mendes,M.L., do Nascimento-Junior,E.M., Reinheimer,D.M. and Martins-Filho,P.R. Efficacy of proteolytic enzyme bromelain on health outcomes after third molar surgery. Systematic review and meta-analysis of randomized clinical trials Med Oral Patol Oral Cir Bucal (2019) 24, e61-e61. PubMed Europe PubMed DOI
Mohamad,N.E., Abu,N., Yeap,S.K. and Alitheen,N.B. Bromelain enhances the anti-tumor effects of cisplatin on 4T1 breast tumor model in vivo Integr Cancer Ther (2019) 18, 1534735419880258-1534735419880258. PubMed Europe PubMed DOI U
Orlandi-Mattos,P.E., Aguiar,R.B., da Silva Vaz I Junior, Moraes,J.Z., de Araujo Carlini,E.L., Juliano,M.A. and Juliano,L. Enkephalin related peptides are released from jejunum wall by orally ingested bromelain Peptides (2019) 115, 32-42. PubMed Europe PubMed DOI
Raeisi,F., Raeisi,E., Heidarian,E., Shahbazi-Gahroui,D. and Lemoigne,Y. Bromelain inhibitory effect on colony formation: an in vitro study on human AGS, PC3, and MCF7 cancer cells J Med Signals Sens (2019) 9, 267-273. PubMed Europe PubMed DOI PMC EPMC U
Taskin,A., Tarakcioglu,M., Ulusal,H., Orkmez,M. and Taysi,S. Idarubicin-bromelain combination sensitizes cancer cells to conventional chemotherapy Iran J Basic Med Sci (2019) 22, 1172-1178. PubMed Europe PubMed DOI PMC EPMC

2018

Ataide,J.A., Gerios,E.F., Mazzola,P.G. and Souto,E.B. Bromelain-loaded nanoparticles: a comprehensive review of the state of the art Adv Colloid Interface Sci (2018) 254, 48-55. PubMed Europe PubMed DOI
Berner,J.E., Keckes,D., Pywell,M. and Dheansa,B. Limitations to the use of bromelain-based enzymatic debridement (NexoBrid((R))) for treating diabetic foot burns: a case series of disappointing results Scars Burn Heal (2018) 4, 2059513118816534-2059513118816534. PubMed Europe PubMed DOI PMC EPMC U
Feng,X., Hang,S., Zhou,Y., Liu,Q. and Yang,H. Bromelain kinetics and mechanism on myofibril from golden pomfret (Trachinotus blochii) J Food Sci (2018) 83, 2148-2158. PubMed Europe PubMed DOI
Jha,I., Bisht,M., Mogha,N.K. and Venkatesu,P. Effect of imidazolium-based ionic liquids on the structure and stability of stem bromelain: concentration and alkyl chain length effect J Phys Chem B (2018) 122, 7522-7529. PubMed Europe PubMed DOI
Kumar,P.K., Bisht,M., Venkatesu,P., Bahadur,I. and Ebenso,E.E. Exploring the effect of choline-based ionic liquids on the stability and activity of stem bromelain J Phys Chem B (2018) 122, 10435-10444. PubMed Europe PubMed DOI
Loo,Y.L., Goh,B.K.L. and Jeffery,S. An overview of the use of bromelain-based enzymatic debridement (Nexobrid(R)) in deep partial and full thickness burns: appraising the evidence J Burn Care Res (2018) 39, 932-938. PubMed Europe PubMed DOI U
Park,S., Oh,J., Kim,M. and Jin,E.J. Bromelain effectively suppresses Kras-mutant colorectal cancer by stimulating ferroptosis Animal Cells Syst (Seoul) (2018) 22, 334-340. PubMed Europe PubMed DOI PMC EPMC U
Rajan,S.G., Sharma,A., Kumari,P. and Biswas,S. Comparative molecular characterization to reveal surface behaviour of non-proteolytic bromelain mutants Curr Pharm Biotechnol (2018) 19, 886-895. PubMed Europe PubMed DOI
Ramli,A.N.M., Manas,N.H.A., Hamid,A.A.A., Hamid,H.A. and Illias,R.M. Comparative structural analysis of fruit and stem bromelain from Ananas comosus Food Chem (2018) 266, 183-191. PubMed Europe PubMed DOI S
Sancesario,G.M., Nuccetelli,M., Cerri,A., Zegeer,J., Severini,C., Ciotti,M.T., Pieri,M., Martorana,A., Caltagirone,C., Nistico,R. and Bernardini,S. Bromelain degrades Abeta1-42 monomers and soluble aggregates: an in vitro study in cerebrospinal fluid of Alzheimer's disease patients Curr Alzheimer Res (2018) 15, 628-636. PubMed Europe PubMed DOI
Schulz,A., Fuchs,P.C., Hans,N., Oplander,C., Valdez,L.B. and Schiefer,J.L. Inhibition of bromelain activity during enzymatic debridement of burn wounds pretreated with frequently used products J Burn Care Res (2018) 39, 413-422. PubMed Europe PubMed DOI
Shoham,Y., Krieger,Y., Tamir,E., Silberstein,E., Bogdanov-Berezovsky,A., Haik,J. and Rosenberg,L. Bromelain-based enzymatic debridement of chronic wounds: a preliminary report Int Wound J (2018) 15, 769-775. PubMed Europe PubMed DOI U
Singer,A.J., Toussaint,J., Chung,W.T., McClain,S.A., Clark,R.A.F., Asculai,E., Geblinger,D. and Rosenberg,L. Development of a contaminated ischemic porcine wound model and the evaluation of bromelain based enzymatic debridement Burns (2018) 44, 896-904. PubMed Europe PubMed DOI U
Soheilifar,S., Bidgoli,M., Hooshyarfard,A., Shahbazi,A., Vahdatinia,F. and Khoshkhooie,F. Effect of oral bromelain on wound healing, pain, and bleeding at donor site following free gingival grafting: a clinical trial J Dent (Tehran) (2018) 15, 309-316. PubMed Europe PubMed PMC EPMC U
Xu,X., Liu,R., Guo,P., Luo,Z., Cai,X., Shu,H., Ge,Y., Chang,C. and Fu,Q. Fabrication of a novel magnetic mesoporous molecularly imprinted polymer based on pericarpium granati-derived carrier for selective absorption of bromelain Food Chem (2018) 256, 91-97. PubMed Europe PubMed DOI

2017

Bhakuni,K. and Venkatesu,P. Crowded milieu tuning the stability and activity of stem bromelain Int J Biol Macromol (2017) 109, 114-123. PubMed Europe PubMed DOI
Hirche,C., Citterio,A., Hoeksema,H., Koller,J., Lehner,M., Martinez,J.R., Monstrey,S., Murray,A., Plock,J.A., Sander,F., Schulz,A., Ziegler,B. and Kneser,U. Eschar removal by bromelain based enzymatic debridement (Nexobrid(R)) in burns: an European consensus Burns (2017) 43, 1640-1653. PubMed Europe PubMed DOI
Luniak,N., Meiser,P., Burkart,S. and Muller,R. Heterologous expression of the plant cysteine protease bromelain and its inhibitor in Pichia pastoris Biotechnol Prog (2017) 33, 54-65. PubMed Europe PubMed DOI E
Matagne,A., Bolle,L., El Mahyaoui,R., Baeyens-Volant,D. and Azarkan,M. The proteolytic system of pineapple stems revisited: purification and characterization of multiple catalytically active forms Phytochemistry (2017) 138, 29-51. PubMed Europe PubMed DOI
Muhammad,Z.A. and Ahmad,T. Therapeutic uses of pineapple-extracted bromelain in surgical care - a review J Pak Med Assoc (2017) 67, 121-125. PubMed Europe PubMed V U
Nor,M.Z.M., Ramchandran,L., Duke,M. and Vasiljevic,T. Integrated ultrafiltration process for the recovery of bromelain from pineapple waste mixture J Food Process Eng (2017) 40, e12492- DOI
Oliveira,C.P., Prado,W.A., Lavayen,V., Buttenbender,S.L., Beckenkamp,A., Martins,B.S., Ludtke,D.S., Campo,L.F., Rodembusch,F.S., Buffon,A., Pessoa,A., Jr., Guterres,S.S. and Pohlmann,A.R. Bromelain-functionalized multiple-wall lipid-core nanocapsules: formulation, chemical structure and antiproliferative effect against human breast cancer cells (MCF-7) Pharm Res (2017) 34, 438-452. PubMed Europe PubMed DOI U
Zaman,M., Zakariya,S.M., Nusrat,S., Khan,M.V., Qadeer,A., Ajmal,M.R. and Khan,R.H. Surfactant-mediated amyloidogenesis behavior of stem bromelain; a biophysical insight J Biomol Struct Dyn (2017) 35, 1407-1419. PubMed Europe PubMed DOI

2016

Belcher,M.D., Kaddour-Djebbar,I., Bollag,W.B. and Davis,L.S. The proteolytic effect of bromelain on bullous pemphigoid antigen-2 J Am Acad Dermatol (2016) 75, 838-840. PubMed Europe PubMed DOI U
Bernela,M., Ahuja,M. and Thakur,R. Enhancement of anti-inflammatory activity of bromelain by its encapsulation in katira gum nanoparticles Carbohydr Polym (2016) 143, 18-24. PubMed Europe PubMed DOI U
Bhatnagar,P., Pant,A.B., Shukla,Y., Panda,A. and Gupta,K.C. Hyaluronic acid grafted PLGA copolymer nanoparticles enhance the targeted delivery of Bromelain in Ehrlich's Ascites Carcinoma Eur J Pharm Biopharm (2016) 105, 176-192. PubMed Europe PubMed DOI U
Bormann,K.H., Weber,K., Kloppenburg,H., Staude,P., Koch,A., Meiser,P. and Gellrich,N.C. Perioperative bromelain therapy after wisdom teeth extraction - a randomized, placebo-controlled, double-blinded, three-armed, cross-over dose-finding study Phytother Res (2016) 30, 2012-2019. PubMed Europe PubMed DOI U
Coelho,D.F., Saturnino,T.P., Fernandes,F.F., Mazzola,P.G., Silveira,E. and Tambourgi,E.B. Azocasein substrate for determination of proteolytic activity: reexamining a traditional method using bromelain samples Biomed Res Int (2016) 2016, 8409183-8409183. PubMed Europe PubMed DOI PMC EPMC A
DaSilva,F.R.P., Vasconcelos,A.C.C.G., Alves,E.H.P., DeOliveira Junior,P.V., DeOliveira,J.S. and Vasconcelos,D.F.P. Bromelain: a potential strategy for the adjuvant treatment of periodontitis Dent Hypotheses (2016) 7, 88-93. DOI U
de Lencastre Novaes,L.C., Jozala,A.F., Lopes,A.M., de Carvalho Santos-Ebinuma,V., Mazzola,P.G. and Pessoa Junior,A. Stability, purification, and applications of bromelain: a review Biotechnol Prog (2016) 32, 5-13. PubMed Europe PubMed DOI
Errasti,M.E., Prospitti,A., Viana,C.A., Gonzalez,M.M., Ramos,M.V., Rotelli,A.E. and Caffini,N.O. Effects on fibrinogen, fibrin, and blood coagulation of proteolytic extracts from fruits of Pseudananas macrodontes, Bromelia balansae, and B. hieronymi (Bromeliaceae) in comparison with bromelain Blood Coagul Fibrinolysis (2016) 27, 441-449. PubMed Europe PubMed DOI
Kasemsuk,T., Saengpetch,N., Sibmooh,N. and Unchern,S. Improved WOMAC score following 16-week treatment with bromelain for knee osteoarthritis Clin Rheumatol (2016) 35, 2531-2540. PubMed Europe PubMed DOI U
Ley,C.M., Ni,Q., Liao,X., Gao,H.L. and Robinson,N. Bromelain and cardiovascular risk factors in diabetes: an exploratory randomized, placebo controlled, double blind clinical trial Chin J Integr Med (2016) 22, 728-737. PubMed Europe PubMed DOI
Li,R., Ding,L., Wu,Z., Wang,Y., Liu,W. and Wang,Y. beta-Cyclodextrin assisted two-stage foam fractionation of bromelain from the crude extract of pineapple peels Ind Crops Prod (2016) 94, 233-239. DOI
Li,Y., Zhou,J., Wang,W. and Wang,J. Bromelain treatment of wheat flour to reduce the allergenicity J Chin Cereals Oils Assoc (2016) 31, 56-60.
Mohan,R., Sivakumar,V., Rangasamy,T. and Muralidharan,C. Optimisation of bromelain enzyme extraction from pineapple (Ananas comosus) and application in process industry Am J Biochem Biotechnol (2016) 12, 188-195. DOI
Muller,A., Barat,S., Chen,X., Bui,K.C., Bozko,P., Malek,N.P. and Plentz,R.R. Comparative study of antitumor effects of bromelain and papain in human cholangiocarcinoma cell lines Int J Oncol (2016) 48, 2025-2034. PubMed Europe PubMed DOI U
Pauzi,A.Z., Yeap,S.K., Abu,N., Lim,K.L., Omar,A.R., Aziz,S.A., Chow,A.L., Subramani,T., Tan,S.G. and Alitheen,N.B. Combination of cisplatin and bromelain exerts synergistic cytotoxic effects against breast cancer cell line MDA-MB-231 in vitro Chin Med (2016) 11, 46-46. PubMed Europe PubMed DOI PMC EPMC U
Rathnavelu,V., Alitheen,N.B., Sohila,S., Kanagesan,S. and Ramesh,R. Potential role of bromelain in clinical and therapeutic applications Biomed Rep (2016) 5, 283-288. PubMed Europe PubMed DOI PMC EPMC U
Rawat,K.A., Singhal,R.K. and Kailasa,S.K. Colorimetric and fluorescence "turn-on" methods for the sensitive detection of bromelain using carbon dots functionalized gold nanoparticles as a dual probe RSC Adv (2016) 6, 32025-32036. DOI A
Rubin,G., Rinott,M., Wolovelsky,A., Rosenberg,L., Shoham,Y. and Rozen,N. A new bromelain-based enzyme for the release of Dupuytren's contracture: Dupuytren's enzymatic bromelain-based release Bone Joint Res (2016) 5, 175-177. PubMed Europe PubMed DOI U
Sharma,K. and Bhattacharyya,D. Application of transverse urea gradient zymography for structural and functional characterization of proteolytic enzymes Curr Sci (2016) 111, 1340-1348. DOI
Shing,C.M., Chong,S., Driller,M.W. and Fell,J.W. Acute protease supplementation effects on muscle damage and recovery across consecutive days of cycle racing Eur J Sport Sci (2016) 16, 206-212. PubMed Europe PubMed DOI
Singh,T., More,V., Fatima,U., Karpe,T., Aleem,M.A. and Prameela,J. Effect of proteolytic enzyme bromelain on pain and swelling after removal of third molars J Int Soc Prev Community Dent (2016) 6, S197-S197. PubMed Europe PubMed DOI PMC EPMC U
Vicente,F.A., Lario,L.D., Pessoa,A. and Ventura,S.P.M. Recovery of bromelain from pineapple stem residues using aqueous micellar two-phase systems with ionic liquids as co-surfactants Process Biochem (2016) 51, 528-534. DOI
Wan,J., Guo,J., Miao,Z. and Guo,X. Reverse micellar extraction of bromelain from pineapple peel - effect of surfactant structure Food Chem (2016) 197, 450-456. PubMed Europe PubMed DOI
Yu,J., Lin,F., Lin,S., Pei,X., Miao,J., Chen,X., Wu,S.G. and Wang,A. A comparative study of papain and bromelain in enzymatic oligomerization of l-Phe methyl ester in aqueous environment J Mol Catal B Enzym (2016) 133, S95-S99. DOI
Zaman,M., Ehtram,A., Chaturvedi,S.K., Nusrat,S. and Khan,R.H. Amyloidogenic behavior of different intermediate state of stem bromelain: a biophysical insight Int J Biol Macromol (2016) 91, 477-485. PubMed Europe PubMed DOI

2015

Arshad,Z.I.M., Amid,A. and Othman,M.E.F. Comparison of different cell disruption methods and cell extractant buffers for recombinant bromelain expressed in E.COLI BL21-A1 J Teknol (2015) 77, 83-87. DOI E
Bhatnagar,P., Pant,A.B., Shukla,Y., Chaudhari,B., Kumar,P. and Gupta,K.C. Bromelain nanoparticles protect against 7,12-dimethylbenz[a]anthracene induced skin carcinogenesis in mouse model Eur J Pharm Biopharm (2015) 91, 35-46. PubMed Europe PubMed DOI
Croisfelt,F., Martins,B.C., Rescolino,R., Coelho,D.F., Zanchetta,B., Mazzola,P.G., Goulart,L.R., Pessoa,A.J., Tambourgi,E.B. and Silveira,E. Poly(N-isopropylacrylamide)-co-acrylamide hydrogels for the controlled release of bromelain from agroindustrial residues of Ananas comosus Planta Med (2015) 81, 1719-1726. PubMed Europe PubMed DOI
Esti,M., Benucci,I., Liburdi,K. and Garzillo,A.M.V. Immobilized pineapple stem bromelain activity in a wine-like medium: effect of inhibitors Food Bioprod Process (2015) 93, 84-89. DOI
Nishikado,H., Fujimura,T., Taka,H., Mineki,R., Ogawa,H., Okumura,K. and Takai,T. Cysteine protease antigens cleave CD123, the alpha subunit of murine IL-3 receptor, on basophils and suppress IL-3-mediated basophil expansion Biochem Biophys Res Commun (2015) 460, 261-266. PubMed Europe PubMed DOI
Rani,A. and Venkatesu,P. Insights into the interactions between enzyme and co-solvents: stability and activity of stem bromelain Int J Biol Macromol (2015) 73C, 189-201. PubMed Europe PubMed DOI
Rosenberg,L., Shoham,Y., Krieger,Y., Rubin,G., Sander,F., Koller,J., David,K., Egosi,D., Ahuja,R. and Singer,A.J. Minimally invasive burn care: a review of seven clinical studies of rapid and selective debridement using a bromelain-based debriding enzyme (Nexobrid(R)) Ann Burns Fire Disasters (2015) 28, 264-274. PubMed Europe PubMed PMC EPMC U
Sahbaz,A., Aynioglu,O., Isik,H., Ozmen,U., Cengil,O., Gun,B.D. and Gungorduk,K. Bromelain: a natural proteolytic for intra-abdominal adhesion prevention Int J Surg (2015) 14C, 7-11. PubMed Europe PubMed DOI U
Zappino,M., Cacciotti,I., Benucci,I., Nanni,F., Liburdi,K., Valentini,F. and Esti,M. Bromelain immobilization on microbial and animal source chitosan films, plasticized with glycerol, for application in wine-like medium: microstructural, mechanical and catalytic characterisations Food Hydrocolloids (2015) 45, 41-47. DOI

2014

Amini,A., Masoumi-Moghaddam,S., Ehteda,A. and Morris,D.L. Bromelain and N-acetylcysteine inhibit proliferation and survival of gastrointestinal cancer cells in vitro: significance of combination therapy J Exp Clin Cancer Res (2014) 33, 92-92. PubMed Europe PubMed DOI PMC EPMC U
Arshad,Z.I., Amid,A., Yusof,F., Jaswir,I., Ahmad,K. and Loke,S.P. Bromelain: an overview of industrial application and purification strategies Appl Microbiol Biotechnol (2014) 98, 7283-7297. PubMed Europe PubMed DOI
Benucci,I., Esti,M. and Liburdi,K. Effect of free and immobilised stem bromelain on protein haze in white wine Austr J Grape Wine Res (2014) 20, 347-352. DOI
Costa,H.B., Fernandes,P.M.B., Romao,W. and Ventura,J.A. A new procedure based on column chromatography to purify bromelain by ion exchange plus gel filtration chromatographies Ind Crops Prod (2014) 59, 163-168. DOI
de Lencastre Novaes,L.C., Jozala,A.F., Mazzola,P.G. and Junior,A.P. The influence of pH, polyethylene glycol and polyacrylic acid on the stability of stem bromelain Bras J Pharm Sci (2014) 50, 371-380. DOI
Dhaneshwar,A.D., Chaurasiya,R.S. and Hebbar,H.U. Process optimization for reverse micellar extraction of stem bromelain with a focus on back extraction Biotechnol Prog (2014) 30, 845-855. PubMed Europe PubMed DOI
George,S., Bhasker,S., Madhav,H., Nair,A. and Chinnamma,M. Functional characterization of recombinant bromelain of Ananas comosus expressed in a prokaryotic system Mol Biotechnol (2014) 56, 166-174. PubMed Europe PubMed DOI E
Majid,O.W. and Al-Mashhadani,B.A. Perioperative bromelain reduces pain and swelling and improves quality of life measures after mandibular third molar surgery: a randomized, double-blind, placebo-controlled clinical trial J Oral Maxillofac Surg (2014) 72, 1043-1048. PubMed Europe PubMed DOI
Medeiros,V., Rainha,N., Paiva,L., Lima,E. and Baptista,J. Bovine milk formula based on partial hydrolysis of caseins by bromelain enzyme: Better digestibility and angiotensin-converting enzyme-inhibitory properties Int J Food Prop (2014) 17, 806-817. DOI
Pillai,K., Ehteda,A., Akhter,J., Chua,T.C. and Morris,D.L. Anticancer effect of bromelain with and without cisplatin or 5-FU on malignant peritoneal mesothelioma cells Anticancer Drugs (2014) 25, 150-160. PubMed Europe PubMed DOI U
Rathnasamy,S. and Kumaresan,R. Comparative studies of bromelain extraction- conventional vs ionic liquid based aqueous two phase extraction Biotechnol An Indian J (2014) 9, 114-119.
Romano,B., Fasolino,I., Pagano,E., Capasso,R., Pace,S., De Rosa,G., Milic,N., Orlando,P., Izzo,A.A. and Borrelli,F. The chemopreventive action of bromelain, from pineapple stem (Ananas comosus L.), on colon carcinogenesis is related to antiproliferative and proapoptotic effects Mol Nutr Food Res (2014) 58, 457-465. PubMed Europe PubMed DOI U
Sharma,H.K., Srivastava,R. and Shukla,S. Isolation, purification and quantitative analysis of cysteine protease, bromelain from Ananas comosus (pineapple) Intl J Pharma Bio Sci (2014) 5, B429-B437.

2013

Aichele,K., Bubel,M., Deubel,G., Pohlemann,T. and Oberringer,M. Bromelain down-regulates myofibroblast differentiation in an in vitro wound healing assay Naunyn Schmiedebergs Arch Pharmacol (2013) 386, 853-863. PubMed Europe PubMed DOI
Amini,A., Ehteda,A., Masoumi Moghaddam,S., Akhter,J., llai,K. and rris,D.L. Cytotoxic effects of bromelain in human gastrointestinal carcinoma cell lines (MKN45, KATO-III, HT29-5F12, and HT29-5M21) Onco Targets Ther (2013) 6, 403-409. PubMed Europe PubMed DOI PMC EPMC U
Banks,J.M., Herman,C.T. and Bailey,R.C. Bromelain decreases neutrophil interactions with p-selectin, but not e-selectin, in vitro by proteolytic cleavage of p-selectin glycoprotein ligand-1 PLoS ONE (2013) 8, e78988-e78988. PubMed Europe PubMed DOI PMC EPMC
Bresolin,I.R.A.P., Bresolin,I.T.L., Silveira,E., Tambourgi,E.B. and Mazzola,P.G. Isolation and purification of bromelain from waste peel of pineapple for therapeutic application Braz Arch Biol Technol (2013) 56, 971-979. DOI
Chaurasiya,R.S. and Umesh Hebbar,H. Extraction of bromelain from pineapple core and purification by RME and precipitation methods Sep Purif Technol (2013) 111, 90-97. DOI
de Lencastre Novaes,L.C., de Carvalho Santos Ebinuma,V., Mazzola,P.G. and Junior,A.P. Polymer-based alternative method to extract bromelain from pineapple peel waste Biotechnol Appl Biochem (2013) 60, 527-535. PubMed Europe PubMed DOI
Fouz,N., Amid,A. and Hashim,Y.Z.H.Y. Cytokinetic study of MCF-7 cells treated with commercial and recombinant bromelain Asian Pac J Cancer Prev (2013) 14, 6709-6714. DOI
Muller,S., Marz,R., Schmolz,M., Drewelow,B., Eschmann,K. and Meiser,P. Placebo-controlled randomized clinical trial on the immunomodulating activities of low- and high-dose bromelain after oral administration - New evidence on the antiinflammatory mode of action of bromelain Phytother Res (2013) 27, 199-204. PubMed Europe PubMed DOI U
Pillai,K., Akhter,J., Chua,T.C. and Morris,D.L. Anticancer property of bromelain with therapeutic potential in malignant peritoneal mesothelioma Cancer Invest (2013) 31, 241-250. PubMed Europe PubMed DOI U
Raimbault,A.K., Zuily-Fodil,Y., Soler,A., Mora,P. and Cruz de Carvalho,M.H. The expression patterns of bromelain and AcCYS1 correlate with blackheart resistance in pineapple fruits submitted to postharvest chilling stress J Plant Physiol (2013) 170, 1442-1446. PubMed Europe PubMed DOI
Rocha,M.V. and Nerli,B.B. Molecular features determining different partitioning patterns of papain and bromelain in aqueous two-phase systems Int J Biol Macromol (2013) 61, 204-211. PubMed Europe PubMed DOI
Secor,E.R., Jr., Szczepanek,S.M., Castater,C.A., Adami,A.J., Matson,A.P., Rafti,E.T., Guernsey,L., Natarajan,P., McNamara,J.T., Schramm,C.M., Thrall,R.S. and Silbart,L.K. Bromelain inhibits allergic sensitization and murine asthma via modulation of dendritic cells Evid Based Complement Alternat Med (2013) 2013, 702196-702196. PubMed Europe PubMed DOI PMC EPMC U

2012

Bromelain (2012) PubMed Europe PubMed
Bala,M., Ismail,N.A., Mel,M., Jami,M.S., MohdSalleh,H. and Amid,A. Bromelain production: current trends and perspective Arch Sci (2012) 65, 369-399. E
Dave,S., Kaur,N.J., Nanduri,R., Dkhar,H.K., Kumar,A. and Gupta,P. Inhibition of adipogenesis and induction of apoptosis and lipolysis by stem bromelain in 3T3-L1 adipocytes PLoS ONE (2012) 7, e30831-e30831. PubMed Europe PubMed DOI PMC EPMC U
Dhandayuthapani,S., Perez,H.D., Paroulek,A., Chinnakkannu,P., Kandalam,U., Jaffe,M. and Rathinavelu,A. Bromelain-induced apoptosis in GI-101A breast cancer cells J Med Food (2012) 15, 344-349. PubMed Europe PubMed DOI U
Foroncewicz,B., Mucha,K., Heidland,A., Ciszek,M., Imiela,J., Helle,F. and Paczek,L. Modulation of serum levels of sRAGE by bromelain in patients with chronic kidney disease: a pilot study Pol Arch Intern Med (2012) 122, 514-516. PubMed Europe PubMed U
Ilaria,B., Marco,E., Katia,L. and Maria Vittoria,G.A. Pineapple stem bromelain immobilized on different supports: catalytic properties in model wine Biotechnol Prog (2012) 28, 1472-1477. PubMed Europe PubMed DOI
Lopez-Garcia,B., Hernandez,M. and Segundo,B.S. Bromelain, a cysteine protease from pineapple (Ananas comosus) stem, is an inhibitor of fungal plant pathogens Lett Appl Microbiol (2012) 55, 62-67. PubMed Europe PubMed DOI U
Mahajan,S., Chandra,V., Dave,S., Nanduri,R. and Gupta,P. Stem bromelain-induced macrophage apoptosis and activation curtail Mycobacterium tuberculosis persistence J Infect Dis (2012) 206, 366-376. PubMed Europe PubMed DOI U
Meiser,P. Bromelain in paediatrics Z Phytother (2012) 33, 210-214. DOI U
Muntari,B., Amid,A., Mel,M., Jami,M.S. and Salleh,H.M. Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology AMB Express (2012) 2, 12-12. PubMed Europe PubMed DOI E
Nadzirah,K.Z., Zainal,S., Noriham,A. and Normah,I. Efficacy of selected purification techniques for bromelain Int Food Res J (2012) 20, 43-46.
Pavan,R., Jain,S., Shraddha and Kumar,A. Properties and therapeutic application of bromelain: a review Biotechnol Res Int (2012) 2012, 976203-976203. PubMed Europe PubMed DOI PMC EPMC
Rosenberg,L., Krieger,Y., Silberstein,E., Arnon,O., Sinelnikov,I.A., Bogdanov-Berezovsky,A. and Singer,A.J. Selectivity of a bromelain based enzymatic debridement agent: a porcine study Burns (2012) 38, 1035-1040. PubMed Europe PubMed DOI U
Rowan,A.D. Stem bromelain [ISSN:978-0-12-407743-0] (2012) 3, 1871-1873. DOI
Secor,E.R., Jr., Shah,S.J., Guernsey,L.A., Schramm,C.M. and Thrall,R.S. Bromelain limits airway inflammation in an ovalbumin-induced murine model of established asthma Altern Ther Health Med (2012) 18, 9-17. PubMed Europe PubMed U
Wang,W., Suguitan,A.L., Jr., Zengel,J., Chen,Z. and Jin,H. Generation of recombinant pandemic H1N1 influenza virus with the HA cleavable by bromelain and identification of the residues influencing HA bromelain cleavage Vaccine (2012) 30, 872-878. PubMed Europe PubMed DOI
Wu,S.Y., Hu,W., Zhang,B., Liu,S., Wang,J.M. and Wang,A.M. Bromelain ameliorates the wound microenvironment and improves the healing of firearm wounds J Surg Res (2012) 176, 503-509. PubMed Europe PubMed DOI U

2011

Dave,S., Mahajan,S., Chandra,V. and Gupta,P. Trifluoroethanol stabilizes the molten globule state and induces non-amyloidic turbidity in stem bromelain near its isoelectric point Int J Biol Macromol (2011) 49, 536-542. PubMed Europe PubMed DOI
Ferreira,J.F., Bresolin,L.R.P., Silveira,E. and Tambourgi,E.B. Purification of bromelain from Ananas comosus by PEG/phosphate ATPS Chem Eng Trans (2011) 24, 931-936. DOI
Johann,K., Eschmann,K. and Meiser,P. [No clinical evidence for an enhanced bleeding tendency due to perioperative treatment with bromelain] Sportverletz Sportschaden (2011) 25, 108-113. PubMed Europe PubMed DOI U
Karlsen,M., Hovden,A.O., Vogelsang,P., Tysnes,B.B. and Appel,S. Bromelain treatment leads to maturation of monocyte-derived dendritic cells but cannot replace PGE2 in a cocktail of IL-1beta, IL-6, TNF-alpha and PGE2 Scand J Immunol (2011) 74, 135-143. PubMed Europe PubMed DOI U
Ley,C.M., Tsiami,A., Ni,Q. and Robinson,N. A review of the use of bromelain in cardiovascular diseases Zhong Xi Yi Jie He Xue Bao (2011) 9, 702-710. PubMed Europe PubMed DOI U
Soares,P., Coelho,D., Mazzola,P., Silveira,E., Carneiro-da-Cunha,M.D.G., Pessoa,A. and Tambourgi,E. Studies on bromelain precipitation by ethanol, poly (ethylene glycol) and ammonium sulphate Chem Eng Trans (2011) 24, 979-984. DOI

2010

Chobotova,K., Vernallis,A.B. and Majid,F.A. Bromelain's activity and potential as an anti-cancer agent: current evidence and perspectives Cancer Lett (2010) 290, 148-156. PubMed Europe PubMed DOI U
Dave,S., Dkhar,H.K., Singh,M.P., Gupta,G., Chandra,V., Mahajan,S. and Gupta,P. Hexafluoroisopropanol-induced helix-sheet transition of stem bromelain: correlation to function Int J Biochem Cell Biol (2010) 42, 938-947. PubMed Europe PubMed DOI
Dave,S., Mahajan,S., Chandra,V., Dkhar,H.K., Sambhavi and Gupta,P. Specific molten globule conformation of stem bromelain at alkaline pH Arch Biochem Biophys (2010) 499, 26-31. PubMed Europe PubMed DOI
Gautam,S.S., Mishra,S.K., Dash,V., Goyal,A.K. and Rath,G. Comparative study of extraction, purification and estimation of bromelain from stem and fruit of pineapple plant Thai J Pharm Sci (2010) 34, 67-76.
Reddy,V.B. and Lerner,E.A. Plant cysteine proteases that evoke itch activate protease-activated receptors Br J Dermatol (2010) 163, 532-535. PubMed Europe PubMed DOI
Singer,A.J., McClain,S.A., Taira,B.R., Rooney,J., Steinhauff,N. and Rosenberg,L. Rapid and selective enzymatic debridement of porcine comb burns with bromelain-derived Debrase: acute-phase preservation of noninjured tissue and zone of stasis J Burn Care Res (2010) 31, 304-309. PubMed Europe PubMed DOI U
Viswanathan,K., Omorebokhae,R., Li,G. and Gross,R.A. Protease-catalyzed oligomerization of hydrophobic amino acid ethyl esters in homogeneous reaction media using l-phenylalanine as a model system Biomacromolecules (2010) 11, 2152-2160. PubMed Europe PubMed DOI
Xue,Y., Wu,C.Y., Branford-White,C.J., Ning,X., Nie,H.L. and Zhu,L.M. Chemical modification of stem bromelain with anhydride groups to enhance its stability and catalytic activity J Mol Catal B Enzym (2010) 63, 188-193. DOI

2009

Ahmad,B., Rathar,G.M., Varshney,A. and Khan,R.H. pH-Dependent urea-induced unfolding of stem bromelain: unusual stability against urea at neutral pH Biochemistry (Mosc) (2009) 74, 1337-1343. PubMed Europe PubMed DOI
Bhattacharya,R. and Bhattacharyya,D. Resistance of bromelain to SDS binding Biochim Biophys Acta (2009) 1794, 698-708. PubMed Europe PubMed DOI
Bhui,K., Prasad,S., George,J. and Shukla,Y. Bromelain inhibits COX-2 expression by blocking the activation of MAPK regulated NF-kappa B against skin tumor-initiation triggering mitochondrial death pathway Cancer Lett (2009) 282, 167-176. PubMed Europe PubMed DOI U
Secor,E.R., Jr., Singh,A., Guernsey,L.A., McNamara,J.T., Zhan,L., Maulik,N. and Thrall,R.S. Bromelain treatment reduces CD25 expression on activated CD4+ T cells in vitro Int Immunopharmacol (2009) 9, 340-346. PubMed Europe PubMed DOI PMC EPMC

2008

Bhattacharyya,B.K. Bromelain: an overview Nat Prod Radiance (2008) 7, 359-363. V
Fitzhugh,D.J., Shan,S., Dewhirst,M.W. and Hale,L.P. Bromelain treatment decreases neutrophil migration to sites of inflammation Clin Immunol (2008) 128, 66-74. PubMed Europe PubMed DOI PMC EPMC
Huang,J.R., Wu,C.C., Hou,R.C. and Jeng,K.C. Bromelain inhibits lipopolysaccharide-induced cytokine production in human THP-1 monocytes via the removal of CD14 Immunol Invest (2008) 37, 263-277. PubMed Europe PubMed DOI
Juhasz,B., Thirunavukkarasu,M., Pant,R., Zhan,L., Penumathsa,S.V., Secor,E.R., Jr., Srivastava,S., Raychaudhuri,U., Menon,V.P., Otani,H., Thrall,R.S. and Maulik,N. Bromelain induces cardioprotection against ischemia-reperfusion injury through Akt/Foxo pathway in rat myocardium Am J Physiol Heart Circ Physiol (2008) 294, H1365-H1370. PubMed Europe PubMed DOI PMC EPMC
Jung,Y.J., Choi,C.S., Park,J.H., Kang,H.W., Choi,J.E., Nou,I.S., Lee,S.Y. and Kang,K.K. Overexpression of the pineapple fruit bromelain gene (BAA) in transgenic Chinese cabbage (Brassica rapa) results in enhanced resistance to bacterial soft rot Electron J Biotechnol (2008) 11
Kalra,N., Bhui,K., Roy,P., Srivastava,S., George,J., Prasad,S. and Shukla,Y. Regulation of p53, nuclear factor kappaB and cyclooxygenase-2 expression by bromelain through targeting mitogen-activated protein kinase pathway in mouse skin Toxicol Appl Pharmacol (2008) 226, 30-37. PubMed Europe PubMed DOI
Onken,J.E., Greer,P.K., Calingaert,B. and Hale,L.P. Bromelain treatment decreases secretion of pro-inflammatory cytokines and chemokines by colon biopsies in vitro Clin Immunol (2008) 126, 345-352. PubMed Europe PubMed DOI PMC EPMC
Sawano,Y., Hatano,K., Miyakawa,T. and Tanokura,M. Absolute side-chain structure at position 13 is required for the inhibitory activity of bromein J Biol Chem (2008) 283, 36338-36343. PubMed Europe PubMed DOI
Secor,E.R., Carson,W.F., Singh,A., Pensa,M., Guernsey,L.A., Schramm,C.M. and Thrall,R.S. Oral bromelain attenuates inflammation in an ovalbumin-induced murine model of asthma Evid Based Complement Alternat Med (2008) 5, 61-69. PubMed Europe PubMed DOI PMC EPMC
Tochi,B.N., Wang,Z., Xu,S.Y. and Zhang,W. Therapeutic application of pineapple protease (bromelain): a review Pak J Nutr (2008) 7, 513-520. V
Umesh Hebbar,H., Sumana,B. and Raghavarao,K.S. Use of reverse micellar systems for the extraction and purification of bromelain from pineapple wastes Bioresour Technol (2008) 99, 4896-4902. PubMed Europe PubMed DOI

2007

Ahmad,B., Shamim,T.A., Haq,S.K. and Khan,R.H. Identification and characterization of functional intermediates of stem bromelain during urea and guanidine hydrochloride unfolding J Biochem (2007) 141, 251-259. PubMed Europe PubMed DOI
Baez,R., Lopes,M.T., Salas,C.E. and Hernandez,M. In vivo antitumoral activity of stem pineapple (Ananas comosus) bromelain Planta Med (2007) 73, 1377-1383. PubMed Europe PubMed DOI
Bahde,R., Palmes,D., Minin,E., Stratmann,U., Diller,R., Haier,J. and Spiegel,H.U. Bromelain ameliorates hepatic microcirculation after warm ischemia J Surg Res (2007) 139, 88-96. PubMed Europe PubMed DOI
Fatima,S. and Khan,R.H. Effect of polyethylene glycols on the function and structure of thiol proteases J Biochem (2007) 142, 65-72. PubMed Europe PubMed DOI
Gupta,P., Maqbool,T. and Saleemuddin,M. Oriented immobilization of stem bromelain via the lone histidine on a metal affinity support J Mol Catal B Enzym (2007) 45, 78-83. DOI
Habib,S., Khan,M.A. and Younus,H. Thermal destabilization of stem bromelain by trehalose Protein J (2007) 26, 117-124. PubMed Europe PubMed DOI
Khatoon,H., Younus,H. and Saleemuddin,M. Stem bromelain: an enzyme that naturally facilitates oriented immobilization Protein Pept Lett (2007) 14, 233-236. PubMed Europe PubMed DOI
Massimiliano,R., Pietro,R., Paolo,S., Sara,P. and Michele,F. Role of bromelain in the treatment of patients with pityriasis lichenoides chronica J Dermatolog Treat (2007) 18, 219-222. PubMed Europe PubMed DOI
Theodorou,L.G., Bieth,J.G. and Papamichael,E.M. The catalytic mode of cysteine proteinases of papain (C1) family Bioresour Technol (2007) 98, 1931-1939. PubMed Europe PubMed DOI
van Kampen,V., Merget,R. and Bruning,T. [Occupational allergies to bromelain] Pneumologie (2007) 61, 159-161. PubMed Europe PubMed DOI
Yamamoto,N., Nakayama,A., Oshima,M., Kawasaki,N. and Aiba,S. Enzymatic hydrolysis of lysine diisocyanate based polyurethanes and segmented polyurethane ureas by various proteases React Funct Polym (2007) 67, 1338-1345. DOI

2006

[YEAR:18-8-2006]Ahmad,B. and Khan,R.H. Studies on the acid unfolded and molten globule states of catalytically active stem bromelain: a comparison with catalytically inactive form J Biochem (18-8-2006) 140, 501-508. PubMed Europe PubMed DOI
[YEAR:12-12-2006]Ahmad,B., Ansari,M.A., Sen,P. and Khan,R.H. Low versus high molecular weight poly (ethylene glycol)-induced states of stem bromelain at low pH: stabilization of molten globule and unfolded states Biopolymers (12-12-2006) 81, 350-359. PubMed Europe PubMed DOI
[YEAR:6-3-2006]Choe,Y., Leonetti,F., Greenbaum,D.C., Lecaille,F., Bogyo,M., Bromme,D., Ellman,J.A. and Craik,C.S. Substrate profiling of cysteine proteases using a combinatorial peptide library identifies functionally unique specificities J Biol Chem (6-3-2006) 281, 12824-12832. PubMed Europe PubMed DOI P
[YEAR:4-7-2006]Grabovac,V. and Bernkop-Schnurch,A. Improvement of the intestinal membrane permeability of low molecular weight heparin by complexation with stem bromelain Int J Pharm (4-7-2006) 326, 153-159. PubMed Europe PubMed DOI
[YEAR:20-12-2006]Hale,L.P., Fitzhugh,D.J. and Staats,H.F. Oral immunogenicity of the plant proteinase bromelain Int Immunopharmacol (20-12-2006) 6, 2038-2046. PubMed Europe PubMed DOI
Orsini,R.A. Bromelain Plast Reconstr Surg (2006) 118, 1640-1644. PubMed Europe PubMed DOI U
[YEAR:29-8-2006]Wen,S., Huang,T.H., Li,G.Q., Yamahara,J., Roufogalis,B.D. and Li,Y. Bromelain improves decrease in defecation in postoperative rats: modulation of colonic gene expression of inducible nitric oxide synthase Life Sci (29-8-2006) 78, 995-1002. PubMed Europe PubMed DOI

2005

[YEAR:17-8-2005]Barth,H., Guseo,A. and Klein,R. In vitro study on the immunological effect of bromelain and trypsin on mononuclear cells from humans Eur J Med Res (17-8-2005) 10, 325-331. PubMed Europe PubMed
Beuth,J. and Braun,J.M. Modulation of murine tumor growth and colonization by bromelaine, an extract of the pineapple plant (Ananas comosum L.) In Vivo (2005) 19, 483-485. PubMed Europe PubMed
Braun,J.M., Schneider,B. and Beuth,H.J. Therapeutic use, efficiency and safety of the proteolytic pineapple enzyme Bromelain-POS in children with acute sinusitis in Germany In Vivo (2005) 19, 417-421. PubMed Europe PubMed
Hale,L.P., Greer,P.K., Trinh,C.T. and James,C.L. Proteinase activity and stability of natural bromelain preparations Int Immunopharmacol (2005) 5, 783-793. PubMed Europe PubMed DOI
Haq,S.K., Rasheedi,S., Sharma,P., Ahmad,B. and Khan,R.H. Influence of salts and alcohols on the conformation of partially folded intermediate of stem bromelain at low pH Int J Biochem Cell Biol (2005) 37, 361-374. PubMed Europe PubMed DOI
Secor,E.R., Jr., Carson,W.F., Cloutier,M.M., Guernsey,L.A., Schramm,C.M., Wu,C.A. and Thrall,R.S. Bromelain exerts anti-inflammatory effects in an ovalbumin-induced murine model of allergic airway disease Cell Immunol (2005) 237, 68-75. PubMed Europe PubMed DOI PMC EPMC

2004

Brien,S., Lewith,G., Walker,A., Hicks,S.M. and Middleton,D. Bromelain as a treatment for osteoarthritis: a review of clinical studies Evid Based Complement Alternat Med (2004) 1, 251-257. PubMed Europe PubMed DOI PMC EPMC
Hale,L.P. Proteolytic activity and immunogenicity of oral bromelain within the gastrointestinal tract of mice Int Immunopharmacol (2004) 4, 255-264. PubMed Europe PubMed DOI
Rowan,A.D. Stem bromelain [ISSN:0-12-079610-4] (2004) 2, 1136-1137. V

2003

Gupta,P., Khan,R.H. and Saleemuddin,M. Induction of 'molten globule' like state in acid-denatured state of unmodified preparation of stem bromelain: implications of disulfides in protein folding Int J Biol Macromol (2003) 33, 167-174. PubMed Europe PubMed DOI
[YEAR:21-3-2003]Gupta,P., Khan,R.H. and Saleemuddin,M. Binding of antibromelain monomeric Fab' improves the stability of stem bromelain against inactivation Biochim Biophys Acta (21-3-2003) 1646, 131-135. PubMed Europe PubMed DOI
[YEAR:15-5-2003]Gupta,P., Khan,R.H. and Saleemuddin,M. Trifluoroethanol-induced "molten globule" state in stem bromelain Arch Biochem Biophys (15-5-2003) 413, 199-206. PubMed Europe PubMed DOI
Rasheedi,S., Haq,S.K. and Khan,R.H. Guanidine hydrochloride denaturation of glycosylated and deglycosylated stem bromelain Biochemistry (Mosc) (2003) 68, 1097-1100. PubMed Europe PubMed DOI

2002

Hale,L.P., Greer,P.K. and Sempowski,G.D. Bromelain treatment alters leukocyte expression of cell surface molecules involved in cellular adhesion and activation Clin Immunol (2002) 104, 183-190. PubMed Europe PubMed DOI
Manhart,N., Akomeah,R., Bergmeister,H., Spittler,A., Ploner,M. and Roth,E. Administration of proteolytic enzymes bromelain and trypsin diminish the number of CD4+ cells and the interferon-gamma response in Peyer's patches and spleen in endotoxemic balb/c mice Cell Immunol (2002) 215, 113-119. PubMed Europe PubMed DOI

2001

[YEAR:25-5-2001]Engwerda,C.R., Andrew,D., Murphy,M. and Mynott,T.L. Bromelain activates murine macrophages and natural killer cells in vitro Cell Immunol (25-5-2001) 210, 5-10. PubMed Europe PubMed DOI
[YEAR:25-5-2001]Engwerda,C.R., Andrew,D., Ladhams,A. and Mynott,T.L. Bromelain modulates T cell and B cell immune responses in vitro and in vivo Cell Immunol (25-5-2001) 210, 66-75. PubMed Europe PubMed DOI
Maurer,H.R. Bromelain: biochemistry, pharmacology and medical use Cell Mol Life Sci (2001) 58, 1234-1245. PubMed Europe PubMed V

1999

[YEAR:1-9-1999]Mynott,T.L., Ladhams,A., Scarmato,P. and Engwerda,C.R. Bromelain, from pineapple stems, proteolytically blocks activation of extracellular regulated kinase-2 in T cells J Immunol (1-9-1999) 163, 2568-2575. PubMed Europe PubMed

1995

Arroyo-Reyna,A. and Hernandez-Arana,A. The thermal denaturation of stem bromelain is consistent with an irreversible two-state model Biochim Biophys Acta (1995) 1248, 123-128. PubMed Europe PubMed DOI

1994

Rowan,A.D. and Buttle,D.J. Pineapple cysteine endopeptidases Methods Enzymol (1994) 244, 555-568. PubMed Europe PubMed DOI V

1990

Rowan,A.D., Christopher,C.W., Kelley,S.F., Buttle,D.J. and Ehrlich,H.P. Debridement of experimental full-thickness skin burns of rats with enzyme fractions derived from pineapple stem Burns (1990) 16, 243-246. PubMed Europe PubMed DOI
Rowan,A.D., Buttle,D.J. and Barrett,A.J. The cysteine proteinases of the pineapple plant Biochem J (1990) 266, 869-875. PubMed Europe PubMed PMC EPMC

1989

Ritonja,A., Rowan,A.D., Buttle,D.J., Rawlings,N.D., Turk,V. and Barrett,A.J. Stem bromelain: amino acid sequence and implications for weak binding of cystatin FEBS Lett (1989) 247, 419-424. PubMed Europe PubMed

1985

Ota,S., Muta,E., Katahira,Y. and Okamoto,Y. Reinvestigation of fractionation and some properties of the proteolytically active components of stem and fruit bromelains J Biochem (1985) 98, 219-228. PubMed Europe PubMed

1983

Carey,P.R., Ozaki,Y. and Storer,A.C. Comparison of the substrate conformations in the active sites of papain, chymopapain, ficin and bromelain by resonance Raman spectroscopy Biochem Biophys Res Commun (1983) 117, 725-731. PubMed Europe PubMed
Lynn,K.R. Definition of the site of reactivity of the ancestral protease of the papain type Phytochemistry (1983) 22, 2485-2487. DOI P

1980

Goto,K., Takahashi,N. and Murachi,T. Structural studies on stem bromelain. Cyanogen bromide cleavage and amino acid sequence of carboxyl-terminal half of the molecule Int J Pept Protein Res (1980) 15, 335-341. PubMed Europe PubMed

1979

Caygill,J.C. Sulphydryl plant proteases Enzyme Microb Technol (1979) 1, 233-242. V

1977

Mineshita,S. and Nagai,Y. Hydrolysis of bradykinin by stem bromelain Jpn J Pharmacol (1977) 27, 170-172. PubMed Europe PubMed P

1976

Cooreman,W.M., Scharpe,S., Demeester,J. and Lauwers,A. Bromelain, biochemical and pharmacological properties Pharm Acta Helv (1976) 51, 73-97. PubMed Europe PubMed
Murachi,T. Bromelain enzymes Methods Enzymol (1976) 45, 475-485. PubMed Europe PubMed DOI V

1975

Shipton,M., Kierstan,M.P.J., Malthouse,J.P.G., Stuchbury,T. and Brocklehurst,K. The case for assigning a value of approximately 4 to pKa(I) of the essential histidine cysteine interactive systems of papain, bromelain and ficin FEBS Lett (1975) 50, 365-368. PubMed Europe PubMed DOI

1973

Perlstein,S.H. and Kezdy,F.J. Isolation and characterization of a protease inhibitor from commercial stem bromelain acetone powder J Supramol Struct (1973) 1, 249-254. PubMed Europe PubMed DOI

1972

Heinicke,R.M., van der Wal,L. and Yokoyama,M. Effect of bromelain (ananase) on human platelet aggregation Experientia (1972) 28, 844-845. PubMed Europe PubMed DOI

1971

Doi,E., Hata,T. and Minami,Y. Fractionation, purification and some properties of proteolytic enzymes from stem bromelain Agric Biol Chem (1971) 35, 1419-1430. DOI

1970

Husain,S.S. and Lowe,G. The amino acid sequence around the active-site cysteine and histidine residues of stem bromelain Biochem J (1970) 117, 341-346. PubMed Europe PubMed PMC EPMC
Murachi,T. [18] Bromelain enzymes Methods Enzymol (1970) 19, 273-284. DOI

1968

Husain,S.S. and Lowe,G. The amino-acid sequence around the active site cysteine and histidine residues of stem-bromelain Chem Commun (London) (1968) 1968, 1387-1389. DOI

1965

Murachi,T., Inagami,T. and Yasui,M. Evidence for alkylphosphorylation of tyrosyl residues of stem bromelain by diisopropylphosphorofluoridate Biochemistry (1965) 4, 2815-2825. PubMed Europe PubMed DOI
Murachi,T. and Yasui,M. Alkylphosphorylation of stem bromelain by diisopropylphosphorofluoridate without inhibition of proteinase activity Biochemistry (1965) 4, 2275-2282. DOI I

1964

Feinstein,G. and Whitaker,J.R. On the molecular weights of the proteolytic enzymes of stem bromelain Biochemistry (1964) 3, 1050-1054. PubMed Europe PubMed
Murachi,T., Yasui,M. and Yasuda,Y. Purification and physical characterization of stem bromelain Biochemistry (1964) 3, 48-55. PubMed Europe PubMed
Murachi,T. Amino acid composition of stem bromelain Biochemistry (1964) 3, 932-934. PubMed Europe PubMed
Ota,S., Moore,S. and Stein,W.H. Preparation and chemical properties of purified stem and fruit bromelains Biochemistry (1964) 3, 180-185. PubMed Europe PubMed

1963

El-Gharbawi,M. and Whitaker,J.R. Fractionation and partial characterization of the proteolytic enzymes of stem bromelain Biochemistry (1963) 2, 476-481. PubMed Europe PubMed
Inagami,T. and Murachi,T. Kinetic studies of bromelain catalysis Biochemistry (1963) 2, 1439-1444. PubMed Europe PubMed

1946

Petermann,M.L. The splitting of human gamma globulin antibodies by papain and bromelin J Am Chem Soc (1946) 68, 106-113. PubMed Europe PubMed

1940

Greenberg,D.M. and Winnick,T. Plant proteases. I. Activation-inhibition reactions J Biol Chem (1940) 135, 761-773.

1939

Berger,J. and Asenjo,C.F. Anthelmintic activity of fresh pineapple juice Science (1939) 90, 299-300. PubMed Europe PubMed DOI