Summary for peptidase M12.150: ruberlysin

Names
MEROPS Name	ruberlysin
Other names	Crotalus ruber (red rattlesnake) metalloendopeptidase II, hemorrhagic toxin II

Domain architecture

MEROPS Classification
Classification	Clan MA >> Subclan MA(M) >> Family M12 >> Subfamily B >> M12.150
Holotype	ruberlysin (Crotalus ruber), Uniprot accession P20897 (peptidase unit: 1-202), MERNUM MER0001124
History	Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.

Activity
Catalytic type	Metallo
NC-IUBMB	Subclass 3.4 (Peptidases) >> Sub-subclass 3.4.24 (Metalloendopeptidases) >> Peptidase 3.4.24.48
Enzymology	BRENDA database
Proteolytic events	CutDB database (1 cleavage)
Physiology	Likely role in snake venom toxicity. Hemorrhagic.
Other databases	PANTHER	http://www.pantherdb.org/panther/familyList.do?searchType=basic&fieldName=all&listType=6&fieldValue=PTHR11905:SF138
Cleavage site specificity	Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern	wy/ydi/h/gphwFls/hmry/lp/gl (based on 16 cleavages)

Specificity matrix

Amino acid	P4	P3	P2	P1	P1'	P2'	P3'	P4'
Gly	1	1	3	7	0	0	2	4
Pro	0	0	0	3	1	0	3	0
Ala	0	1	0	1	0	0	0	1
Val	1	1	0	0	0	2	0	1
Leu	1	0	1	0	6	0	4	3
Ile	0	3	1	0	0	0	0	0
Met	0	0	0	0	0	3	0	0
Phe	0	0	0	0	7	2	0	0
Tyr	3	4	3	1	0	3	1	0
Trp	3	0	0	2	0	0	0	0
Ser	0	0	1	0	2	0	0	0
Thr	0	0	0	0	0	0	0	1
Cys	0	0	0	0	0	0	0	0
Asn	0	0	0	0	0	0	0	0
Gln	0	0	0	0	0	0	0	0
Asp	0	3	0	0	0	0	0	0
Glu	1	1	1	0	0	0	1	0
Lys	0	0	0	0	0	0	0	0
Arg	0	0	1	0	0	3	0	0
His	0	0	5	2	0	3	1	0