Family M32
Summary for family M32
| Name | Peptidase family M32 (carboxypeptidase Taq family) |
|---|
| Family type peptidase | M32.001 - carboxypeptidase Taq (Thermus aquaticus), MEROPS Accession MER0001186 (peptidase unit: 1-511) |
| Content of family | Peptidase family M32 contains metallocarboxypeptidases. |
| History |
Identifier created: Methods Enzymol. 248:183-228 (1995)
|
| Catalytic type | Metallo |
| Active site residues | H276 E277 H280 E306 |
| Active site | Family M32 members contain two zinc-binding histidines and a catalytic glutamate in an HEXXH zinc-binding motif - a motif that is most commonly found in zinc endopeptidases and aminopeptidases. The third zinc ligand is a glutamate found in an HES motif further toward the C-terminus. |
| Activities and specificities | Carboxypeptidase Taq (M32.001) and carboxypeptidase Pfu (M32.002) are both stable up to high temperatures, and exhibit carboxypeptidase activity with broad specificities, except (in the case of carboxypeptidase Taq) when there is a proline at the C-terminus. Carboxypeptidase Taq (M32.001) hydrolyses amino acids with long side chains most readily (Motoshima & Kaminogawa, 2004). |
| Molecular structure | The structure of carboxypeptidase Pfu is homodimeric with each subunit being mostly helical (Arndt et al., 2002). The HEXXH motif is found at the bottom of a deep substrate binding groove formed by the two subunits. The structure was found to be to be similar to that of neurolysin (M03.002) (Arndt et al., 2002) and angiotensin-converting enzyme 2 (M02.004) (Natesh et al., 2003). |
| Clan | MA |
| Subclan | MA(E) |
| Basis of clan assignment | Predicted active site residues for members of this family and thermolysin, the type example for clan MA, occur in the motif HEXXH |
| Peptidases and Homologues |
MEROPS ID |
Structure |
| carboxypeptidase Taq | M32.001 | - |
| carboxypeptidase Pfu | M32.002 | Yes |
| TcMCP1 carboxypeptidase | M32.003 | Yes |
| TcMCP2 carboxypeptidase (Trypanosoma cruzi) | M32.004 | - |
| LmaCP1 carboxypeptidase (Leishmania major) and similar | M32.005 | - |
| ypwA peptidase | M32.006 | Yes |
| Family M32 non-peptidase homologues | non-peptidase homologue | - |
| Family M32 unassigned peptidases | unassigned | Yes |
