Electron Microscopy Data Bank

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LM18/Nb136 bispecific tetra-nanobody immunoglobulin in complex with SARS-CoV-2-6P-Mut7 S protein (focused refinement)

Release date: June 14, 2023
Resolution: 3.34 Å
EM Method: Single-particle
Fitted PDBs: 8dt8
Q-score: 0.443

Misson Mindrebo L, Liu H, Ozorowski G, Tran Q, Woehl J, Khalek I, Smith JM, Barman S, Zhao F, Keating C, Limbo O, Verma M, Liu J, Stanfield RL, Zhu X, Turner HL, Sok D, Huang PS, Burton DR, Ward AB, Wilson IA, Jardine JG

PNAS (2023) 120 pp. e2216612120-e2216612120 [ Pubmed: 37276407 DOI: doi:10.1073/pnas.2216612120 ]

Sample:

2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Lm18 nanobody (14 kDa, Protein from synthetic construct)
Lm18/nb136 bispecific tetra-nanobody immunoglobulin in complex with sars-cov-2-6p-mut7 s protein (Complex from Severe acute respiratory syndrome coronavirus 2)
Spike glycoprotein (141 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
Nb136 nanobody (13 kDa, Protein from synthetic construct)

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EMD-27693

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LM18/Nb136 bispecific tetra-nanobody immunoglobulin in complex with SARS-CoV-2-6P-Mut7 S protein (global refinement)

Release date: June 14, 2023
Resolution: 3.14 Å
EM Method: Single-particle

Misson Mindrebo L, Liu H, Ozorowski G, Tran Q, Woehl J, Khalek I, Smith JM, Barman S, Zhao F, Keating C, Limbo O, Verma M, Liu J, Stanfield RL, Zhu X, Turner HL, Sok D, Huang PS, Burton DR, Ward AB, Wilson IA, Jardine JG

PNAS (2023) 120 pp. e2216612120-e2216612120 [ Pubmed: 37276407 DOI: doi:10.1073/pnas.2216612120 ]

Sample:

Lm18/nb136 bispecific tetra-nanobody immunoglobulin in complex with sars-cov-2-6p-mut7 s protein (Complex from Severe acute respiratory syndrome coronavirus 2)

EMD-26928

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Negative stain map of cH4/3

Release date: June 22, 2022
Resolution: 20.0 Å
EM Method: Single-particle

Zhu X, Han J, Sun W, Puente-Massaguer E, Yu W, Palese P, Krammer F, Ward AB, Wilson IA

PNAS (2022) 119 pp. e2200821119-e2200821119 [ DOI: doi:10.1073/pnas.2200821119 Pubmed: 35594401 ]

Sample:

Negative stain map of ch4/3 (Cellular component from Influenza A virus)

EMD-26929

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Negative stain half map of cH4/3 state 1

Release date: June 22, 2022
Resolution: 20.0 Å
EM Method: Single-particle

Zhu X, Han J, Sun W, Puente-Massaguer E, Yu W, Palese P, Krammer F, Ward AB, Wilson IA

PNAS (2022) 119 pp. e2200821119-e2200821119 [ DOI: doi:10.1073/pnas.2200821119 Pubmed: 35594401 ]

Sample:

Negative stain map of ch4/3 state 1 (Cellular component from Influenza A virus)

EMD-26930

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Negative stain map of cH4/3 state 3

Release date: June 22, 2022
Resolution: 20.0 Å
EM Method: Single-particle

Zhu X, Han J, Sun W, Puente-Massaguer E, Yu W, Palese P, Krammer F, Ward AB, Wilson IA

PNAS (2022) 119 pp. e2200821119-e2200821119 [ DOI: doi:10.1073/pnas.2200821119 Pubmed: 35594401 ]

Sample:

Negative stain map of ch4/3 state 2 (Cellular component from Influenza A virus)

EMD-26931

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Negative stain map of cH4/3 state 3

Release date: June 22, 2022
Resolution: 20.0 Å
EM Method: Single-particle

Zhu X, Han J, Sun W, Puente-Massaguer E, Yu W, Palese P, Krammer F, Ward AB, Wilson IA

PNAS (2022) 119 pp. e2200821119-e2200821119 [ DOI: doi:10.1073/pnas.2200821119 Pubmed: 35594401 ]

Sample:

Negative stain map of ch4/3 state 3 (Cellular component from Influenza A virus)

EMD-26932

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Negative stain map of cH4/3 state 4

Release date: June 22, 2022
Resolution: 20.0 Å
EM Method: Single-particle

Zhu X, Han J, Sun W, Puente-Massaguer E, Yu W, Palese P, Krammer F, Ward AB, Wilson IA

PNAS (2022) 119 pp. e2200821119-e2200821119 [ DOI: doi:10.1073/pnas.2200821119 Pubmed: 35594401 ]

Sample:

Negative stain map of ch4/3 state 4 (Cellular component from Influenza A virus)

EMD-26933

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Negative stain map of cH15/3

Release date: June 22, 2022
Resolution: 20.0 Å
EM Method: Single-particle

Zhu X, Han J, Sun W, Puente-Massaguer E, Yu W, Palese P, Krammer F, Ward AB, Wilson IA

PNAS (2022) 119 pp. e2200821119-e2200821119 [ DOI: doi:10.1073/pnas.2200821119 Pubmed: 35594401 ]

Sample:

Negative stain map of ch15/3 (Cellular component from Influenza A virus)

EMD-26934

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Negative stain map of cH15/3 state 1

Release date: June 22, 2022
Resolution: 20.0 Å
EM Method: Single-particle

Zhu X, Han J, Sun W, Puente-Massaguer E, Yu W, Palese P, Krammer F, Ward AB, Wilson IA

PNAS (2022) 119 pp. e2200821119-e2200821119 [ DOI: doi:10.1073/pnas.2200821119 Pubmed: 35594401 ]

Sample:

Negative stain map of ch15/3 state 1 (Cellular component from Influenza A virus)

EMD-26935

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Negative stain map of cH15/3 state 2

Release date: June 22, 2022
Resolution: 20.0 Å
EM Method: Single-particle

Zhu X, Han J, Sun W, Puente-Massaguer E, Yu W, Palese P, Krammer F, Ward AB, Wilson IA

PNAS (2022) 119 pp. e2200821119-e2200821119 [ DOI: doi:10.1073/pnas.2200821119 Pubmed: 35594401 ]

Sample:

Negative stain map of ch15/3 state 2 (Cellular component from Influenza A virus)

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