Structure of alpha1B and betaI/IVb microtubule bound to GMPCPP
bioRxiv (2023) [ DOI: doi:10.1101/2023.12.01.569594 ]
- Tubulin alpha-1b chain (50 kDa, Protein from Homo sapiens)
- Alpha1b and betai/ivb microtubule (300 kDa, Complex from Homo sapiens)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
Mahalingan KK, Grotjahn DA, Li Y, Lander GC, Zehr EA, Roll-Mecak A
Nat Chem Biol (2024) [ DOI: doi:10.1038/s41589-024-01599-0 Pubmed: 38658656 ]
- Tubulin beta-i/ivb chain (Protein from Homo sapiens)
- Tubulin alpha-1b chain (Protein from Homo sapiens)
- Ttll6 bound to unmodified human microtubules (300 kDa, Complex)
- Tubulin alpha-1b, betai and betaivb (Complex from Homo sapiens)
- Tubulin polyglutamylase ttll6 (Complex from Mus musculus)
- Tubulin polyglutamylase ttll6 (Protein from Mus musculus)
Map of Tubulin Tyrosine Ligase Like 6
Mahalingan KK, Grotjahn DA, Li Y, Lander GC, Zehr EA, Roll-Mecak A
Nat Chem Biol (2024) [ DOI: doi:10.1038/s41589-024-01599-0 Pubmed: 38658656 ]
Composite map of TTLL6 bound to unmodified human microtubule
Mahalingan KK, Grotjahn DA, Li Y, Lander GC, Zehr EA, Roll-Mecak A
Nat Chem Biol (2024) [ DOI: doi:10.1038/s41589-024-01599-0 Pubmed: 38658656 ]
- Tubulin alpha-1b chain (Macromolecule from Homo sapiens)
- Tubulin beta chain (Macromolecule from Homo sapiens)
- Ttll6 bound to unmodified human microtubule (100 kDa, Complex)
- Tubulin polyglutamylase ttll6 in complex with unmodified human microtubule (Complex from Mus musculus)
- Alpha1b and betai/betaivb microtubule (Complex from Homo sapiens)
- Tubulin polyglutamylase ttll6 (Macromolecule from Mus musculus)
Cryo-EM micrographs of human α1B/βI+βIVb microtubules bound to GMPCPP decorated with TTLL6
Mahalingan KK, Grotjahn DA, Li Y, Lander GC, Zehr EA, Roll-Mecak A
Nat Cell Biol (2024) [ DOI: 10.1038/s41589-024-01599-0 Pubmed: 38658656 ]
Image sets:
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8t42
Q-score: 0.395
Mahalingan KK, Grotjahn DA, Li Y, Lander GC, Zehr EA, Roll-Mecak A
Nat Chem Biol (2024) [ DOI: doi:10.1038/s41589-024-01599-0 Pubmed: 38658656 ]
- Tubulin alpha-1b chain (50 kDa, Protein from Homo sapiens)
- Tubulin alpha-1b chain, tubulin betai chain (Complex from Homo sapiens)
- Tubulin polyglutamylase ttll6 (59 kDa, Protein from Mus musculus)
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Homo sapiens)
- Ttll6 bound to unmodified human microtubules (300 kDa, Complex)
- Tubulin polyglutamylase ttll6 (Complex from Mus musculus)
Katanin hexamer in the spiral conformation in complex with substrate
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6ugd
Q-score: 0.506
Zehr EA, Szyk A, Szczesna E, Roll-Mecak A
Dev Cell (2020) 52 pp. 118-131.e6 [ DOI: doi:10.1016/j.devcel.2019.10.010 Pubmed: 31735665 ]
- Meiotic spindle formation protein mei-1 (53 kDa, Protein from Caenorhabditis elegans)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Hexameric complex of c.elegans katanin bound to polyglutamate peptide (312 kDa, Complex from Caenorhabditis elegans)
- Polyglutamate peptide (1 kDa, Protein from synthetic construct)
- Magnesium ion (24 Da, Ligand)
Katanin hexamer in the ring conformation in complex with substrate
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6uge
Q-score: 0.452
Zehr EA, Szyk A, Szczesna E, Roll-Mecak A
Dev Cell (2020) 52 pp. 118-131.e6 [ DOI: doi:10.1016/j.devcel.2019.10.010 Pubmed: 31735665 ]
- Meiotic spindle formation protein mei-1 (53 kDa, Protein from Caenorhabditis elegans)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Polyglutamate peptide (1 kDa, Protein from synthetic construct)
- Hexameric complex of c.elegans katanin bound to polyglutamate peptide (312 kDa, Complex from Caenorhabditis elegans)
- Magnesium ion (24 Da, Ligand)
Katanin hexamer in the ring conformation with resolved protomer one in complex with substrate
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 6ugf
Q-score: 0.392
Zehr EA, Szyk A, Szczesna E, Roll-Mecak A
Dev Cell (2020) 52 pp. 118-131.e6 [ DOI: doi:10.1016/j.devcel.2019.10.010 Pubmed: 31735665 ]
- Meiotic spindle formation protein mei-1 (53 kDa, Protein from Caenorhabditis elegans)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Polyglutamate peptide (1 kDa, Protein from synthetic construct)
- Hexameric complex of c.elegans katanin bound to polyglutamate peptide (312 kDa, Complex from Caenorhabditis elegans)
- Magnesium ion (24 Da, Ligand)
Spastin hexamer in complex with substrate
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6p07
Q-score: 0.462
Sandate CR, Szyk A, Zehr EA, Lander GC, Roll-Mecak A
Nat Struct Mol Biol (2019) 26 pp. 671-678 [ DOI: doi:10.1038/s41594-019-0257-3 Pubmed: 31285604 ]
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Spastin (54 kDa, Protein from Drosophila melanogaster)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Polyglutamate peptide (1 kDa, Protein from Drosophila melanogaster)
- Complex of spastin homohexamer bound to polyglutmate peptide (294 MDa, Complex from Drosophila melanogaster)