6uge

Electron Microscopy
3.6Å resolution

Katanin hexamer in the ring conformation in complex with substrate

Released:
Entry authors: Zehr EA, Roll-Mecak A
Related structures: EMD-20762

Function and Biology Details

Reaction catalysed:
n ATP + n H(2)O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Meiotic spindle formation protein mei-1 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 490 amino acids
Theoretical weight: 53.58 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P34808 (Residues: 1-472; Coverage: 100%)
Gene names: T01G9.5, mei-1
Sequence domains:
Polyglutamate peptide Chain: G
Molecule details ›
Chain: G
Length: 12 amino acids
Theoretical weight: 1.57 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-20762
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided