Cryo electron micrographs of Pol delta-DNA-PCNA giving rise to various PCNA tilt angles
Lancey C, Tehseen M, Raducanu VS, Rashid F, Merino N, Ragan TJ, Savva CG, Zaher MS, Shirbini A, Blanco FJ, Hamdan SM, De Biasio A
Nat Commun (2020) 11 [ DOI: 10.1038/s41467-020-14898-6 Pubmed: 32111820 ]
Image sets:
- Cryo-electron micrographs of Pol delta holoenzyme giving rise to different tilted conformers, collection 1
- Cryo-electron micrographs of Pol delta holoenzyme giving rise to different tilted conformers, collection 2
- Cryo-electron micrographs of Pol delta holoenzyme giving rise to different tilted conformers, collection 3
Cryo electron micrographs of Pol delta-PCNA-DNA-FEN1 sample
Lancey C, Tehseen M, Raducanu VS, Rashid F, Merino N, Ragan TJ, Savva CG, Zaher MS, Shirbini A, Blanco FJ, Hamdan SM, De Biasio A
Nat Commun (2020) 11 [ DOI: 10.1038/s41467-020-14898-6 Pubmed: 32111820 ]
Image sets:
Human polymerase delta holoenzyme Conformer 1
Resolution: 4.27 Å
EM Method: Single-particle
Fitted PDBs: 6s1m
Q-score: 0.239
Lancey C, Tehseen M, Raducanu VS, Rashid F, Merino N, Ragan TJ, Savva CG, Zaher MS, Shirbini A, Blanco FJ, Hamdan SM, De Biasio A
Nat Commun (2020) 11 pp. 1109-1109 [ Pubmed: 32111820 DOI: doi:10.1038/s41467-020-14898-6 ]
- Proliferating cell nuclear antigen (29 kDa, Protein from Homo sapiens)
- Dna primer (7 kDa, DNA from synthetic construct)
- Pold holoenzyme (Complex)
- Iron/sulfur cluster (351 Da, Ligand)
- Dna polymerase delta subunit 3 (52 kDa, Protein from Homo sapiens)
- Dna polymerase delta subunit 2 (51 kDa, Protein from Homo sapiens)
- Dna (Complex from synthetic construct)
- Dna polymerase delta subunit 4 (16 kDa, Protein from Homo sapiens)
- Thymidine-5'-triphosphate (482 Da, Ligand)
- Human polymerase delta (Complex from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Dna template (11 kDa, DNA from synthetic construct)
- Proliferating cell nuclear antigen (Complex from Homo sapiens)
- Dna polymerase delta catalytic subunit (123 kDa, Protein from Homo sapiens)
Human polymerase delta holoenzyme Conformer 2
Resolution: 4.86 Å
EM Method: Single-particle
Fitted PDBs: 6s1n
Q-score: 0.162
Lancey C, Tehseen M, Raducanu VS, Rashid F, Merino N, Ragan TJ, Savva CG, Zaher MS, Shirbini A, Blanco FJ, Hamdan SM, De Biasio A
Nat Commun (2020) 11 pp. 1109-1109 [ Pubmed: 32111820 DOI: doi:10.1038/s41467-020-14898-6 ]
- Proliferating cell nuclear antigen (29 kDa, Protein from Homo sapiens)
- Dna primer (7 kDa, DNA from synthetic construct)
- Iron/sulfur cluster (351 Da, Ligand)
- Pold holoenzyme (Complex)
- Dna polymerase delta subunit 3 (52 kDa, Protein from Homo sapiens)
- Dna polymerase delta subunit 2 (51 kDa, Protein from Homo sapiens)
- Dna (Complex from synthetic construct)
- Dna polymerase delta subunit 4 (16 kDa, Protein from Homo sapiens)
- Thymidine-5'-triphosphate (482 Da, Ligand)
- Human polymerase delta (Complex from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Dna template (11 kDa, DNA from synthetic construct)
- Proliferating cell nuclear antigen (Complex from Homo sapiens)
- Dna polymerase delta catalytic subunit (123 kDa, Protein from Homo sapiens)
Human polymerase delta holoenzyme Conformer 3
Resolution: 8.1 Å
EM Method: Single-particle
Fitted PDBs: 6s1o
Q-score: 0.094
Lancey C, Tehseen M, Raducanu VS, Rashid F, Merino N, Ragan TJ, Savva CG, Zaher MS, Shirbini A, Blanco FJ, Hamdan SM, De Biasio A
Nat Commun (2020) 11 pp. 1109-1109 [ Pubmed: 32111820 DOI: doi:10.1038/s41467-020-14898-6 ]
- Proliferating cell nuclear antigen (29 kDa, Protein from Homo sapiens)
- Dna primer (7 kDa, DNA from synthetic construct)
- Iron/sulfur cluster (351 Da, Ligand)
- Pold holoenzyme (Complex)
- Dna polymerase delta subunit 3 (52 kDa, Protein from Homo sapiens)
- Dna polymerase delta subunit 2 (51 kDa, Protein from Homo sapiens)
- Dna (Complex from synthetic construct)
- Dna polymerase delta subunit 4 (16 kDa, Protein from Homo sapiens)
- Thymidine-5'-triphosphate (482 Da, Ligand)
- Human polymerase delta (Complex from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Dna template (11 kDa, DNA from synthetic construct)
- Proliferating cell nuclear antigen (Complex from Homo sapiens)
- Dna polymerase delta catalytic subunit (123 kDa, Protein from Homo sapiens)
Processive human polymerase delta holoenzyme
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 6tny
Q-score: 0.442
Lancey C, Tehseen M, Raducanu VS, Rashid F, Merino N, Ragan TJ, Savva CG, Zaher MS, Shirbini A, Blanco FJ, Hamdan SM, De Biasio A
Nat Commun (2020) 11 pp. 1109-1109 [ Pubmed: 32111820 DOI: doi:10.1038/s41467-020-14898-6 ]
- Proliferating cell nuclear antigen (29 kDa, Protein from Homo sapiens)
- Dna primer (7 kDa, DNA from synthetic construct)
- Iron/sulfur cluster (351 Da, Ligand)
- Dna primer, template (Complex from synthetic construct)
- Dna polymerase delta subunit 3 (52 kDa, Protein from Homo sapiens)
- Dna polymerase delta subunit 2 (51 kDa, Protein from Homo sapiens)
- Dna polymerase delta subunit 4 (16 kDa, Protein from Homo sapiens)
- Toolbelt (Complex)
- Thymidine-5'-triphosphate (482 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Dna template (11 kDa, DNA from synthetic construct)
- Dna polymerase (Complex from Homo sapiens)
- Proliferating cell nuclear antigen (Complex from Homo sapiens)
- Dna polymerase delta catalytic subunit (123 kDa, Protein from Homo sapiens)
Human polymerase delta-FEN1-PCNA toolbelt
Resolution: 4.05 Å
EM Method: Single-particle
Fitted PDBs: 6tnz
Q-score: 0.283
Lancey C, Tehseen M, Raducanu VS, Rashid F, Merino N, Ragan TJ, Savva CG, Zaher MS, Shirbini A, Blanco FJ, Hamdan SM, De Biasio A
Nat Commun (2020) 11 pp. 1109-1109 [ Pubmed: 32111820 DOI: doi:10.1038/s41467-020-14898-6 ]
- Proliferating cell nuclear antigen (29 kDa, Protein from Homo sapiens)
- Dna primer (7 kDa, DNA from synthetic construct)
- Iron/sulfur cluster (351 Da, Ligand)
- Dna primer, template (Complex from synthetic construct)
- Dna polymerase delta subunit 3 (52 kDa, Protein from Homo sapiens)
- Dna polymerase delta subunit 2 (51 kDa, Protein from Homo sapiens)
- Dna polymerase delta subunit 4 (16 kDa, Protein from Homo sapiens)
- Toolbelt (Complex)
- Thymidine-5'-triphosphate (482 Da, Ligand)
- Flap endonuclease 1 (42 kDa, Protein from Homo sapiens)
- Dna template (11 kDa, DNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Proliferating cell nuclear antigen, flap endonuclease (Complex from Homo sapiens)
- Dna polymerase (Complex from Homo sapiens)
- Dna polymerase delta catalytic subunit (123 kDa, Protein from Homo sapiens)