6tnz

Electron Microscopy
4.05Å resolution

Human polymerase delta-FEN1-PCNA toolbelt

Released:
Source organism: Homo sapiens
Related structures: EMD-10540

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Entry contents:
6 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (8 distinct):
DNA polymerase delta catalytic subunit Chain: A
Molecule details ›
Chain: A
Length: 1107 amino acids
Theoretical weight: 123.79 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P28340 (Residues: 1-1107; Coverage: 100%)
Gene names: POLD, POLD1
Sequence domains:
DNA polymerase delta subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 469 amino acids
Theoretical weight: 51.34 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P49005 (Residues: 1-469; Coverage: 100%)
Gene name: POLD2
Sequence domains:
DNA polymerase delta subunit 3 Chain: C
Molecule details ›
Chain: C
Length: 474 amino acids
Theoretical weight: 52.53 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q15054 (Residues: 2-466; Coverage: 100%)
Gene names: KIAA0039, POLD3
Sequence domains: DNA polymerase subunit Cdc27
DNA polymerase delta subunit 4 Chain: D
Molecule details ›
Chain: D
Length: 137 amino acids
Theoretical weight: 16.27 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9HCU8 (Residues: 2-107; Coverage: 99%)
Gene names: POLD4, POLDS
Sequence domains: DNA polymerase delta, subunit 4
Proliferating cell nuclear antigen Chains: E, F, G
Molecule details ›
Chains: E, F, G
Length: 264 amino acids
Theoretical weight: 29.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12004 (Residues: 1-261; Coverage: 100%)
Gene name: PCNA
Sequence domains:
Flap endonuclease 1 Chain: H
Molecule details ›
Chain: H
Length: 380 amino acids
Theoretical weight: 42.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P39748 (Residues: 1-380; Coverage: 100%)
Gene names: FEN1, RAD2
Sequence domains:
DNA primer Chain: P
Molecule details ›
Chain: P
Length: 25 nucleotides
Theoretical weight: 7.67 KDa
DNA template Chain: T
Molecule details ›
Chain: T
Length: 38 nucleotides
Theoretical weight: 11.68 KDa

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution: 4.05Å
Relevant EMDB volumes: EMD-10540
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli BL21(DE3)