Konduru2020 - Genome-scale metabolic reconstruction and in silico analysis of rice leaf blight pathogen, Xanthomonas oryzae

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Model Identifier
MODEL1912100001
Short description
Genome-scale metabolic reconstruction and in silico analysis of rice leaf blight pathogen, Xanthomonas oryzae
Format
SBML (L3V1)
Related Publication
  • Koduru2020 - Genome-scale metabolic reconstruction and in silico analysis of rice leaf blight pathogen, Xanthomonas oryzae
  • Lokanand Koduru, Hyang Yeon Kim, Meiyappan Lakshmanan, Bijayalaxmi Mohanty, Yi Qing Lee, Choong Hwan Lee, Dong-Yup Lee
  • Molecular plant pathology , 1/ 2020
  • Sungkyunkwan University
  • Xanthomonas oryzae pathovar oryzae (Xoo) is a vascular pathogen that causes leaf blight in rice leading to severe yield losses. Since the usage of chemical control methods has not been very promising for the future disease management, it is of high importance to systematically gain new insights about Xoo virulence and pathogenesis, and devise effective strategies to combat the rice disease. To do so, we newly reconstructed a genome-scale metabolic model of Xoo (iXOO673) and validated the model predictions using culture experiments. Comparison of the metabolic architecture of Xoo and other plant pathogens found that Entner-Doudoroff pathway is a more common feature in these bacteria than previously thought, while suggesting some of the unique virulence mechanisms related to Xoo metabolism. Subsequent constraint-based flux analysis allowed us to show that Xoo modulates fluxes through gluconeogenesis, glycogen biosynthesis and degradation pathways, thereby exacerbating the leaf blight in rice exposed to nitrogenous fertilizers, which is remarkably consistent with published experimental literature. Moreover, model-based interrogation of transcriptomic data revealed the metabolic components under the diffusible signal factor (DSF) regulon that are crucial for virulence and survival in Xoo. Finally, we identified promising antibacterial targets for the control of leaf blight in rice by resorting to gene essentiality analysis.
Contributors
Lokanand, Rahuman Sheriff

Metadata information

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Mathematical Modelling Ontology Constraint-based model

Curation status
Non-curated

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Model files

Xanthomonas_oryzae_KACC_10331.xml Xanthomonas oryzae SBML Level 3 Version 1 2.32 MB Preview | Download

  • Model originally submitted by : Lokanand
  • Submitted: 21-Jan-2020 22:40:34
  • Last Modified: 21-Jan-2020 22:40:34
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  • Version: 7 public model Download this version
    • Submitted on: 21-Jan-2020 22:40:34
    • Submitted by: Rahuman Sheriff
    • With comment: Edited model metadata online.
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