Dong2018 - E2F dynamics in G1 phase
The length of the G1 phase in the cell cycle shows significant variability in different cell types and tissue types. To gain insights into the control of G1 length, we generated an E2F activity reporter that captures free E2F activity after dissociation from Rb sequestration and followed its kinetics of activation at the single-cell level, in real time. Our results demonstrate that its activity is precisely coordinated with S phase progression. Quantitative analysis indicates that there is a pre-S phase delay between E2F transcriptional dynamic and activity dynamics. This delay is variable among different cell types and is strongly modulated by the cyclin D/CDK4/6 complex activity through Rb phosphorylation. Our findings suggest that the main function of this complex is to regulate the appropriate timing of G1 length.
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Cyclin D/CDK4/6 activity controls G1 length in mammalian cells.
- Dong P, Zhang C, Parker BT, You L, Mathey-Prevot B
- PloS one , 1/ 2018 , Volume 13 , Issue 1 , pages: e0185637 , PubMed ID: 29309421
- Department of Pharmacology and Cancer Biology, Duke University School of Medicine, Durham, NC, United States of America.
- The length of the G1 phase in the cell cycle shows significant variability in different cell types and tissue types. To gain insights into the control of G1 length, we generated an E2F activity reporter that captures free E2F activity after dissociation from Rb sequestration and followed its kinetics of activation at the single-cell level, in real time. Our results demonstrate that its activity is precisely coordinated with S phase progression. Quantitative analysis indicates that there is a pre-S phase delay between E2F transcriptional dynamic and activity dynamics. This delay is variable among different cell types and is strongly modulated by the cyclin D/CDK4/6 complex activity through Rb phosphorylation. Our findings suggest that the main function of this complex is to regulate the appropriate timing of G1 length.
Submitter of this revision: Ashley Xavier
Modellers: Ashley Xavier
Metadata information
hasTaxon (2 statements)
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| Name | Description | Size | Actions |
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Model files |
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| Dong2018_final.xml | SBML lvl2 file containing the model | 87.61 KB | Preview | Download |
Additional files |
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| Dong2018.cps | copasi file to generate figure 3 g | 137.82 KB | Preview | Download |
- Model originally submitted by : Ashley Xavier
- Submitted: Nov 7, 2018 1:26:16 PM
- Last Modified: Nov 7, 2018 1:26:16 PM
Revisions
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Version: 6
- Submitted on: Nov 7, 2018 1:26:16 PM
- Submitted by: Ashley Xavier
- With comment: Edited model metadata online.
(added: 05 Nov 2018, 16:56:18, updated: 22 Jan 2019, 17:20:06)