Giantsos-Adams2013 - Growth of glycocalyx under static conditions

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Model Identifier
MODEL1609100001
Short description
Giantsos-Adams2013 - Growth of glycocalyx under static conditions

This model is described in the article:

Giantsos-Adams KM, Koo AJ, Song S, Sakai J, Sankaran J, Shin JH, Garcia-Cardena G, Dewey CF.
Cell Mol Bioeng 2013 Jun; 6(2): 160-174

Abstract:

The local hemodynamic shear stress waveforms present in an artery dictate the endothelial cell phenotype. The observed decrease of the apical glycocalyx layer on the endothelium in atheroprone regions of the circulation suggests that the glycocalyx may have a central role in determining atherosclerotic plaque formation. However, the kinetics for the cells' ability to adapt its glycocalyx to the environment have not been quantitatively resolved. Here we report that the heparan sulfate component of the glycocalyx of HUVECs increases by 1.4-fold following the onset of high shear stress, compared to static cultured cells, with a time constant of 19 h. Cell morphology experiments show that 12 h are required for the cells to elongate, but only after 36 h have the cells reached maximal alignment to the flow vector. Our findings demonstrate that following enzymatic degradation, heparan sulfate is restored to the cell surface within 12 h under flow whereas the time required is 20 h under static conditions. We also propose a model describing the contribution of endocytosis and exocytosis to apical heparan sulfate expression. The change in HS regrowth kinetics from static to high-shear EC phenotype implies a differential in the rate of endocytic and exocytic membrane turnover.

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SBML (L2V4)
Related Publication
  • Heparan Sulfate Regrowth Profiles Under Laminar Shear Flow Following Enzymatic Degradation.
  • Giantsos-Adams KM, Koo AJ, Song S, Sakai J, Sankaran J, Shin JH, Garcia-Cardena G, Dewey CF Jr
  • Cellular and molecular bioengineering , 6/ 2013 , Volume 6 , pages: 160-174 , PubMed ID: 23805169
  • Department of Mechanical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Rm. 3-254, Cambridge, MA 02139 USA ; Department of Mechanical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Rm. 3-254, Cambridge, MA 02139 USA.
  • The local hemodynamic shear stress waveforms present in an artery dictate the endothelial cell phenotype. The observed decrease of the apical glycocalyx layer on the endothelium in atheroprone regions of the circulation suggests that the glycocalyx may have a central role in determining atherosclerotic plaque formation. However, the kinetics for the cells' ability to adapt its glycocalyx to the environment have not been quantitatively resolved. Here we report that the heparan sulfate component of the glycocalyx of HUVECs increases by 1.4-fold following the onset of high shear stress, compared to static cultured cells, with a time constant of 19 h. Cell morphology experiments show that 12 h are required for the cells to elongate, but only after 36 h have the cells reached maximal alignment to the flow vector. Our findings demonstrate that following enzymatic degradation, heparan sulfate is restored to the cell surface within 12 h under flow whereas the time required is 20 h under static conditions. We also propose a model describing the contribution of endocytosis and exocytosis to apical heparan sulfate expression. The change in HS regrowth kinetics from static to high-shear EC phenotype implies a differential in the rate of endocytic and exocytic membrane turnover.
Contributors
Kristina Giantsos-Adams

Metadata information

hasProperty
Mathematical Modelling Ontology Ordinary differential equation model

Curation status
Non-curated


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  • Model originally submitted by : Kristina Giantsos-Adams
  • Submitted: Sep 10, 2016 1:58:15 AM
  • Last Modified: Sep 28, 2016 1:47:42 PM
Revisions
  • Version: 2 public model Download this version
    • Submitted on: Sep 28, 2016 1:47:42 PM
    • Submitted by: Kristina Giantsos-Adams
    • With comment: Current version of Giantsos-Adams2013 - Growth of glycocalyx under static conditions
  • Version: 1 public model Download this version
    • Submitted on: Sep 10, 2016 1:58:15 AM
    • Submitted by: Kristina Giantsos-Adams
    • With comment: Original import of MODEL1609100001.xml.origin
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