This a model from the article:
Critical study of and improvements in chromatographic methods for the analysis of type B trichothecenes.
Mateo JJ, Llorens A, Mateo R, Jimenez M. J Chromatogr A 2001 May 18;918(1):99-112 11403460 ,
Various analytical methods used in the analysis of type B trichothecenes (deoxynivalenol, nivalenol, 3- and 15-acetyldeoxynivalenol) in cereals were compared and optimised in this work. These methods use either GC-electron-capture detection (ECD) of trimethylsilyl, trifluoroacetyl and heptafluorobutyryl derivatives or HPLC with UV or photodiode array detection of analytes. A new HPLC procedure using fluorescence detection prior derivatisation with coumarin-3-carbonyl chloride has been also tested. Five extraction solvents and two solid-phase extraction cartridges (silica, Florisil) plus a especial clean-up column (MycoSep 225) were compared in order to obtain the best recovery of the mycotoxins with minimal presence of coextractives in the chromatograms. The chosen extraction solvent was a mixture of acetonitrile-water (84:16, v/v). The MycoSep 225 column was chosen as the best alternative for clean-up of grain samples. For GC-ECD analysis, derivatisation of analytes with heptafluorobutyric anhydride prior the final determination was chosen as the most suitable procedure. HPLC-photodiode array (at 221 nm) analysis was more suitable for determination of type B trichothecenes than HPLC of the fluorescent coumarin-3-carbonyl derivatives. Recoveries obtained in spiked corn, rice and wheat are reported. The utility of the proposed methodology was assayed in cereal cultures of various Fusarium strains.
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The original model was: Mateo JJ, Llorens A, Mateo R, Jimenez M. (2001) - version=1.0
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- Robust oscillations within the interlocked feedback model of Drosophila circadian rhythm.
- Ueda HR, Hagiwara M, Kitano H
- Journal of theoretical biology , 6/ 2001 , Volume 210 , pages: 401-406 , PubMed ID: 11403560
- ERATO Kitano Symbiotic Systems Group, Suite 6A, M31, 6-31-15 Jinguumae, Shibuya, Tokyo, 150-0001, Japan. firstname.lastname@example.org
- A mechanism for generating circadian rhythms has been of major interest in recent years. After the discovery of per and tim, a model with a simple feedback loop involving per and tim has been proposed. However, it is recognized that the simple feedback model cannot account for phenotypes generated by various mutants. A recent report by Glossop, Lyons & Hardin [Science286, 766 (1999)] on Drosophila suggests involvement of another feedback loop by dClk that is interlocked with per-tim feedback loop. In order to examine whether interlocked feedback loops can be a basic mechanism for circadian rhythms, a mathematical model was created and examined. Through extensive simulation and mathematical analysis, it was revealed that the interlocked feedback model accounts for the observations that are not explained by the simple feedback model. Moreover, the interlocked feedback model has robust properties in oscillations.
Submitter of this revision: Camille Laibe
Modellers: Camille Laibe
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- Model originally submitted by : Camille Laibe
- Submitted: Jun 23, 2010 10:12:10 AM
- Last Modified: Jun 25, 2010 2:01:14 PM
- Submitted on: Jun 25, 2010 2:01:14 PM
- Submitted by: Camille Laibe
- With comment: Current version of Ueda2001_typeBtrichothecenes
- Submitted on: Jun 23, 2010 10:12:10 AM
- Submitted by: Camille Laibe
- With comment: Original import of Ueda2001_typeBtrichothecenes
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