Aubry1995 - Nine-compartment model of fluid-phase endocytosis kinetics in Dictyostelium discoideum

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Model Identifier
BIOMD0000000987
Short description
Fluid-phase endoeytosis (pinocytosis) kinetics were studied in Dictyostelium discoideum amoebae from the axenic strain Ax-2 that exhibits high rates of fluid-phase endoeytosis when cultured in liquid nutrient media. Fluorescein-labelled dextran (FITC-dextran) was used as a marker in continuous uptake- and in pulse-chase exocytosis experiments. In the latter case, efflux of the marker was monitored on cells loaded for short periods of time and resuspended in marker-free medium. A multicompartmental model was developed which describes satisfactorily fluid-phase endocytosis kinetics. In particular, it accounts correctly for the extended latency period before exocytosis in pulse-chase experiments and it suggests the existence of some sorts of maturation stages in the pathway.
Format
SBML (L2V4)
Related Publication
  • Modelling of fluid-phase endocytosis kinetics in the amoebae of the cellular slime mouldDictyostelium discoideum. A multicompartmental approach
  • Laurence Aubry, Gérard Klein, Jean-Louis Martiel, Michel Satre
  • Acta Biotheoretica , 12/ 1995 , Volume 43 , Issue 4 , pages: 319--333 , DOI: 10.1007/BF00713556
  • Laboratoire de Biologie Cellulaire, URA 1130 CNRS, CEA, Département de Biologie Moléculaire et Structurale, Centre d'Etudes Nucléaires, 17, rue des Martyrs, 38054, Grenoble Cedex 9, France
  • Fluid-phase endocytosis (pinocytosis) kinetics were studied inDictyostelium discoideum amoebae from the axenic strain Ax-2 that exhibits high rates of fluid-phase endocytosis when cultured in liquid nutrient media. Fluorescein-labelled dextran (FITC-dextran) was used as a marker in continuous uptake- and in pulse-chase exocytosis experiments. In the latter case, efflux of the marker was monitored on cells loaded for short periods of time and resuspended in marker-free medium. A multicompartmental model was developed which describes satisfactorily fluid-phase endocytosis kinetics. In particular, it accounts correctly for the extended latency period before exocytosis in pulse-chase experiments and it suggests the existence of some sorts of maturation stages in the pathway.
Contributors
Kausthubh Ramachandran

Metadata information

hasProperty
Mathematical Modelling Ontology Ordinary differential equation model
isDescribedBy
hasTaxon

Curation status
Curated


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Model files

Aubry1995.xml SBML L2V4 file of nine-compartmental model of fluid-phase endocytosis kinetics in Dictystelium discoideum 53.11 KB Preview | Download

Additional files

Aubry1995.cps COPASI 4.29 (Build 228) file of nine-compartmental model of fluid-phase endocytosis kinetics in Dictystelium discoideum 81.95 KB Preview | Download
Aubry1995.omex COMBINE archive of nine-compartmental model of fluid-phase endocytosis kinetics in Dictystelium discoideum 11.92 KB Preview | Download
Aubry1995.sedml SED-ML file of nine-compartmental model of fluid-phase endocytosis kinetics in Dictystelium discoideum 1.82 KB Preview | Download

  • Model originally submitted by : Kausthubh Ramachandran
  • Submitted: Feb 10, 2021 9:54:23 AM
  • Last Modified: Feb 10, 2021 9:54:23 AM
Revisions
  • Version: 2 public model Download this version
    • Submitted on: Feb 10, 2021 9:54:23 AM
    • Submitted by: Kausthubh Ramachandran
    • With comment: Automatically added model identifier BIOMD0000000987
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Curator's comment:
(added: 10 Feb 2021, 09:52:17, updated: 10 Feb 2021, 09:52:17)
Fig 5 A from the manuscript is reproduced here with parameters as given in the corresponding figure legend. The y-axis units are units of marker fluid per cell.