Queralt2006 - Initiation of mitotic exit by downregulation of PP2A in budding yeast

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Model Identifier
BIOMD0000000953
Short description

Mathematical model of mitotic exit in budding yeast.

Format
SBML (L2V4)
Related Publication
  • Downregulation of PP2A(Cdc55) phosphatase by separase initiates mitotic exit in budding yeast.
  • Queralt E, Lehane C, Novak B, Uhlmann F
  • Cell , 5/ 2006 , Volume 125 , pages: 719-732 , PubMed ID: 16713564
  • Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, Lincoln's Inn Fields Laboratories, 44 Lincoln's Inn Fields, London WC2A 3PX, United Kingdom.
  • After anaphase, the high mitotic cyclin-dependent kinase (Cdk) activity is downregulated to promote exit from mitosis. To this end, in the budding yeast S. cerevisiae, the Cdk counteracting phosphatase Cdc14 is activated. In metaphase, Cdc14 is kept inactive in the nucleolus by its inhibitor Net1. During anaphase, Cdk- and Polo-dependent phosphorylation of Net1 is thought to release active Cdc14. How Net1 is phosphorylated specifically in anaphase, when mitotic kinase activity starts to decline, has remained unexplained. Here, we show that PP2A(Cdc55) phosphatase keeps Net1 underphosphorylated in metaphase. The sister chromatid-separating protease separase, activated at anaphase onset, interacts with and downregulates PP2A(Cdc55), thereby facilitating Cdk-dependent Net1 phosphorylation. PP2A(Cdc55) downregulation also promotes phosphorylation of Bfa1, contributing to activation of the "mitotic exit network" that sustains Cdc14 as Cdk activity declines. These findings allow us to present a new quantitative model for mitotic exit in budding yeast.
Contributors
Matthieu MAIRE, Krishna Kumar Tiwari

Metadata information

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Mathematical Modelling Ontology Ordinary differential equation model
unknownQualifier
Mathematical Modelling Ontology Ordinary differential equation model

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Curated


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Model files

queralt2006_final.xml SBML L2V4 representation of Queralt2006 - Initiation of mitotic exit by downregulation of PP2A in budding yeast 157.41 KB Preview | Download

Additional files

queralt2006_final.sedml SEDML file for reproducing figure 7B in the reference publication. 5.97 KB Preview | Download
queralt2006_final.cps Copasi file (version 4.23 Build 184) for reproducing figure 7B in the reference publication. 215.33 KB Preview | Download

  • Model originally submitted by : Matthieu MAIRE
  • Submitted: 06-Sep-2018 13:39:56
  • Last Modified: 18-May-2020 12:22:02
Revisions
  • Version: 4 public model Download this version
    • Submitted on: 18-May-2020 12:22:02
    • Submitted by: Krishna Kumar Tiwari
    • With comment: Automatically added model identifier BIOMD0000000953
  • Version: 2 public model Download this version
    • Submitted on: 06-Sep-2018 13:39:56
    • Submitted by: Matthieu MAIRE
    • With comment: Edited model metadata online.
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Curator's comment:
(added: 06 Sep 2018, 14:00:16, updated: 06 Sep 2018, 14:00:16)
Figure 7B (bottom) of the reference publication has been reproduced using Copasi 4.23 (Build 184). Use attached SEDML file to reproduce figure 7B.