Koo2013 - Shear stress induced eNOS expression - Model 3

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  • In silico modeling of shear-stress-induced nitric oxide production in endothelial cells through systems biology.
  • Koo A, Nordsletten D, Umeton R, Yankama B, Ayyadurai S, García-Cardeña G, Dewey CF Jr
  • Biophysical journal , 5/ 2013 , Volume 104 , pages: 2295-2306 , PubMed ID: 23708369
  • Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Nitric oxide (NO) produced by vascular endothelial cells is a potent vasodilator and an antiinflammatory mediator. Regulating production of endothelial-derived NO is a complex undertaking, involving multiple signaling and genetic pathways that are activated by diverse humoral and biomechanical stimuli. To gain a thorough understanding of the rich diversity of responses observed experimentally, it is necessary to account for an ensemble of these pathways acting simultaneously. In this article, we have assembled four quantitative molecular pathways previously proposed for shear-stress-induced NO production. In these pathways, endothelial NO synthase is activated 1), via calcium release, 2), via phosphorylation reactions, and 3), via enhanced protein expression. To these activation pathways, we have added a fourth, a pathway describing actual NO production from endothelial NO synthase and its various protein partners. These pathways were combined and simulated using CytoSolve, a computational environment for combining independent pathway calculations. The integrated model is able to describe the experimentally observed change in NO production with time after the application of fluid shear stress. This model can also be used to predict the specific effects on the system after interventional pharmacological or genetic changes. Importantly, this model reflects the up-to-date understanding of the NO system, providing a platform upon which information can be aggregated in an additive way.
Andrew Koo

Metadata information

BioModels Database MODEL1302180005
BioModels Database BIOMD0000000466
PubMed 23708369
Taxonomy Homo sapiens

Curation status

Original model(s)

Name Description Size Actions

Model files

BIOMD0000000466_url.xml SBML L2V4 representation of Koo2013 - Shear stress induced eNOS expression - Model 3 191.85 KB Preview | Download

Additional files

BIOMD0000000466.m Auto-generated Octave file 13.99 KB Preview | Download
BIOMD0000000466_urn.xml Auto-generated SBML file with URNs 190.76 KB Preview | Download
BIOMD0000000466-biopax2.owl Auto-generated BioPAX (Level 2) 43.63 KB Preview | Download
BIOMD0000000466-biopax3.owl Auto-generated BioPAX (Level 3) 69.15 KB Preview | Download
BIOMD0000000466.svg Auto-generated Reaction graph (SVG) 95.75 KB Preview | Download
BIOMD0000000466.pdf Auto-generated PDF file 303.27 KB Preview | Download
BIOMD0000000466.png Auto-generated Reaction graph (PNG) 421.44 KB Preview | Download
BIOMD0000000466.xpp Auto-generated XPP file 9.68 KB Preview | Download
BIOMD0000000466.vcml Auto-generated VCML file 86.74 KB Preview | Download
BIOMD0000000466.sci Auto-generated Scilab file 205.00 Bytes Preview | Download

  • Model originally submitted by : Andrew Koo
  • Submitted: 18-Feb-2013 20:27:51
  • Last Modified: 07-Apr-2014 04:00:36
  • Version: 2 public model Download this version
    • Submitted on: 07-Apr-2014 04:00:36
    • Submitted by: Andrew Koo
    • With comment: Current version of Koo2013 - Shear stress induced eNOS expression - Model 3
  • Version: 1 public model Download this version
    • Submitted on: 18-Feb-2013 20:27:51
    • Submitted by: Andrew Koo
    • With comment: Original import of BIOMD0000000466.xml.origin

(*) You might be seeing discontinuous revisions as only public revisions are displayed here. Any private revisions unpublished model revision of this model will only be shown to the submitter and their collaborators.

: Variable used inside SBML models

Reactions Rate Parameters
s42 => s107; s44, s44, s42 s44*s42*k21/(Km21+s42) k21=4.0E-5 inv_sec; Km21=25.0 substance
s100 => s43; s101, s100, s101 k15*s100*s101/(Km15+s100) Km15=15.0 substance; k15=0.005 inv_sec
s104 => s44; s43, s104, s43 k19*s104*s43/(Km19+s104) Km19=30.0 substance; k19=0.002 inv_sec
s37 => s51; s106, s106 V27*s106/(s106+Km27) Km27=16.0 substance; V27=0.02824 nM_inv_s
s98 => s91; s98 V8*s98/(Km8+s98) V8=154.0 nM_inv_s; Km8=340.0 substance
s95 => s93; s39, s119, s39 exp(unity-(s39/tf)^0.35)*normal*((s39+tiny_num)/unimol)^-0.65*(unity-(s39/tf)^0.35) unimol=1.0 substance; normal=4.0 nM_inv_s; tiny_num=1.0E-6 substance; tf=60.0 substance; unity=1.0 dimensionless
s113 => s114 + s93; s113, s93, s114 k6*s113-kr6*s93*s114 k6=2040.0 inv_sec; kr6=15700.0 inv_nM_s
s100 => s96; s100 V14*s100/(s100+Km14) Km14=15.0 substance; V14=0.375 nM_inv_s
s101 => s97; s101 V12*s101/(Km12+s101) V12=0.125 nM_inv_s; Km12=8.0 substance
s99 => s104; s43, s99, s43 k17*s99*s43/(s99+Km17) k17=0.002 inv_sec; Km17=30.0 substance
s96 => s100; s101, s101, s96 k13*s101*s96/(Km13+s96) Km13=15.0 substance; k13=0.005 inv_sec
s102 => s103; s102 V10*s102/(Km10+s102) Km10=0.0571 substance; V10=0.289 nM_inv_s
Curator's comment:
(added: 19 Aug 2013, 13:57:38, updated: 19 Aug 2013, 13:57:38)
The paper describes four sub-models and an integrated version of the four sub-models. This model corresponds to Model 3 - Shear stress induced eNOS expression. Figure 3C of the paper is reproduced here using Copasi 4.10 (Build 55).