EMD-9999 Experiments and Validation

Cryo-EM structure of human MLL1-ubNCP complex (4.0 angstrom)

Single particle reconstruction
Overview of EMD-9999
Sample name: Human MLL1 complex associated with an H2B-monoubiquitinated nucleosome (4.0 angstrom)
Organisms: Homo sapiens, Xenopus laevis, synthetic construct
Fitted atomic model: 6kiv
Related EM entries by publication: EMD-0693, EMD-0694, EMD-0695, EMD-9998

Map parameters

Recommended contour level: 0.011 (author)
Number of grid points: 270 × 270 × 270
Voxel size: 1.09 × 1.09 × 1.09 Å
Minimum density: -0.208
Maximum density: 0.348
Average density: -0.00
Standard deviation: 0.012

Sample information

Sample name: Human MLL1 complex associated with an H2B-monoubiquitinated nucleosome (4.0 angstrom)
Ligands: S-ADENOSYL-L-HOMOCYSTEINE, ZINC ION
Nucleic acids: DNA (145-MER), DNA (145-MER)
Proteins: Human MLL1 complex associated with an H2B-monoubiquitinated nucleosome (4.0 angstrom), Human MLL1 complex, H2B-monoubiquitinated nucleosome, Histone H3, Histone H4, Histone H2A, Histone H2B 1.1, Histone-lysine N-methyltransferase 2A, Retinoblastoma-binding protein 5, Ubiquitin, Set1/Ash2 histone methyltransferase complex subunit ASH2, WD repeat-containing protein 5

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Number of particles: 127898

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 40.0
Holder model: OTHER

Specimen

Preparation
Specimen state: particle
Vitrification
Cryogen: ETHANE