EMDB EMD-9998

Single particle reconstruction
3.2Å resolution

Cryo-EM structure of human MLL1-ubNCP complex (3.2 angstrom)

Map released:
Last modified:
Overview of EMD-9998
Source organisms:
Fitted atomic model: 6kiu
Related EM entries by publication: EMD-0693, EMD-0694, EMD-0695, EMD-9999
Primary publication:
Structural basis of nucleosome recognition and modification by MLL methyltransferases.
Xue H, Yao T, Cao M, Zhu G, Li Y, Yuan G, Chen Y, Lei M, Huang J
Nature 573 445-449 (2019)
PMID: 31485071

Function and Biology Details

Sample name: Human MLL1 complex associated with an H2B-monoubiquitinated nucleosome (3.2 angstrom)
Ligands: S-ADENOSYL-L-HOMOCYSTEINE, ZINC ION, LYSINE, GLUTAMINE
Nucleic acids: DNA (145-MER), DNA (145-MER)
Proteins: Human MLL1 complex associated with an H2B-monoubiquitinated nucleosome (3.2 angstrom), Human MLL1, H2B-monoubiquitinated nucleosome, Histone H3, Histone H4, Histone H2A, Histone H2B 1.1, Histone-lysine N-methyltransferase 2A, Retinoblastoma-binding protein 5, WD repeat-containing protein 5, Set1/Ash2 histone methyltransferase complex subunit ASH2, Ubiquitin

Experimental Information Details

Resolution: 3.2Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Microscope: FEI TITAN KRIOS
Detector: OTHER