EMDB EMD-9916

Single particle reconstruction
3.79Å resolution

CryoEM structure of sigma appropriation complex

Map released:
Overview of EMD-9916
Source organisms:
Fitted atomic model: 6k4y
Primary publication:
Structural basis of sigma appropriation
Shi J, Wen A, ZHAO M, YOU L, ZHANG Y, Feng Y
NUCLEIC ACIDS RES. - (2019)

Function and Biology Details

Sample name: sigma appropriation complex
Ligands: MAGNESIUM ION, ZINC ION
Nucleic acids: DNA (60-MER), DNA (60-MER)
Proteins: sigma appropriation complex, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', DNA-directed RNA polymerase subunit omega, RNA polymerase sigma factor RpoD, 10 kDa anti-sigma factor, Middle transcription regulatory protein motA

Experimental Information Details

Resolution: 3.79Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)