EMDB EMD-9876

Single particle reconstruction
3.1Å resolution

Structure of PaaZ, a bifunctional enzyme in complex with NADP+ and CCoA

Map released:
Overview of EMD-9876
Source organism: Escherichia coli K-12 [83333]
Fitted atomic model: 6jqo
Related EM entries by publication: EMD-9873, EMD-9874, EMD-9875
Primary publication:
Molecular basis for metabolite channeling in a ring opening enzyme of the phenylacetate degradation pathway
Gakher L, Vinothkumar KR, Katagihallimath N, Sowdhamini R, Sathyanarayanan N, Cannone G
Nat Commun - (2019)

Function and Biology Details

Sample name: PaaZ
Ligands: NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, CROTONYL COENZYME A
Proteins: PaaZ, Bifunctional protein PaaZ

Experimental Information Details

Resolution: 3.1Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C3
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON III (4k x 4k)