EMDB EMD-9852

Single particle reconstruction
4.08Å resolution

Cryo-EM structure of a Q-engaged arrested complex

Map released:
Overview of EMD-9852
Source organisms:
Fitted atomic model: 6jnx
Primary publication:
Structural basis of Q-dependent transcription antitermination
Shi J, Gao X, Tian T, Yu Z, Gao B, Wen A, Chang S, You L, Zhang Y, Zhang X, Feng Y
Nat Commun - (2019)

Function and Biology Details

Sample name: 21Q-engaged arrested complex
Ligands: MAGNESIUM ION, ZINC ION
Nucleic acids: DNA (63-MER), RNA (5'-R(P*AP*UP*AP*AP*GP*GP*UP*GP*GP*GP*GP*UP*UP*AP*GP*UP*GP*A)-3'), DNA (63-MER)
Proteins: 21Q-engaged arrested complex, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', DNA-directed RNA polymerase subunit omega, RNA polymerase sigma factor RpoD, Antiterminator Q protein

Experimental Information Details

Resolution: 4.08Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)