Single particle reconstruction
6.8Å resolution

cryo-EM structure of DOT1L bound to H2B ubiquitinated nucleosome

Map released:
Last modified:
Overview of EMD-9844
Source organisms:
Fitted atomic model: 6jma
Related EM entry by publication: EMD-9843
Primary publication:
Structural basis of recognition and destabilization of the histone H2B ubiquitinated nucleosome by the DOT1L histone H3 Lys79 methyltransferase.
Jang S, Kang C, Yang HS, Jung T, Hebert H, Chung KY, Kim SJ, Hohng S, Song JJ
Genes Dev. - (2019)
PMID: 30923167

Function and Biology Details

Sample name: DOT1L bound to H2B ubiquitinated nucleosome
Nucleic acid: DNA I&J
Proteins: DOT1L bound to H2B ubiquitinated nucleosome, Histone H3.2, Histone H4, Histone H2A, Histone H2B 1.1, Histone-lysine N-methyltransferase, H3 lysine-79 specific, Ubiquitin

Experimental Information Details

Resolution: 6.8Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN
Detector: GATAN K2 SUMMIT (4k x 4k)