EMDB EMD-9843

Single particle reconstruction
7.3Å resolution

cryo-EM structure of DOT1L bound to unmodified nucleosome

Map released:
Last modified:
Overview of EMD-9843
Source organisms:
Fitted atomic model: 6jm9
Related EM entry by publication: EMD-9844
Primary publication:
Structural basis of recognition and destabilization of the histone H2B ubiquitinated nucleosome by the DOT1L histone H3 Lys79 methyltransferase.
Jang S, Kang C, Yang HS, Jung T, Hebert H, Chung KY, Kim SJ, Hohng S, Song JJ
Genes Dev. - (2019)
PMID: 30923167

Function and Biology Details

Sample name: DOT1L bound to unmodified nucleosome
Ligand: S-ADENOSYLMETHIONINE
Nucleic acids: DNA strand I, DNA strand J
Proteins: DOT1L bound to unmodified nucleosome, Histone H3.2, Histone H4, Histone H2A, Histone H2B 1.1, Histone-lysine N-methyltransferase, H3 lysine-79 specific

Experimental Information Details

Resolution: 7.3Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN
Detector: GATAN K2 SUMMIT (4k x 4k)