EMDB EMD-9777

Single particle reconstruction
3.8Å resolution

Structure of C2S2M2-type PSII-FCPII supercomplex from diatom

Map released:
Last modified:
Overview of EMD-9777
Source organism: Chaetoceros gracilis [184592]
Fitted atomic model: 6j40
Related EM entry by publication: EMD-9775, EMD-9776
Primary publication:
Structural basis for energy harvesting and dissipation in a diatom PSII-FCPII supercomplex.
Nagao R, Kato K, Suzuki T, Ifuku K, Uchiyama I, Kashino Y, Dohmae N, Akimoto S, Shen JR, Miyazaki N, Akita F
Nat.Plants - (2019)
PMID: 31358960

Function and Biology Details

Sample name: C2S2M2-type PSII-FCPII supercomplex
Ligands: FE (II) ION, CHLOROPHYLL A, PHEOPHYTIN A, BETA-CAROTENE, 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL, BICARBONATE ION, 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE, 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE, CA-MN4-O5 CLUSTER, DIGALACTOSYL DIACYL GLYCEROL (DGDG), 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE, PROTOPORPHYRIN IX CONTAINING FE, (3S,3'S,5R,5'R,6S,6'R,8'R)-3,5'-dihydroxy-8-oxo-6',7'-didehydro-5,5',6,6',7,8-hexahydro-5,6-epoxy-beta,beta-caroten-3'-yl acetate, DODECYL-ALPHA-D-MALTOSIDE
Proteins: C2S2M2-type PSII-FCPII supercomplex, Photosystem II reaction center protein D1, Photosystem II chlorophyll protein CP47, Photosystem II chlorophyll protein CP43, Photosystem II reaction center protein D2, Cytochrome b559 subunit alpha, Cytochrome b559 subunit beta, Photosystem II reaction center protein H, Photosystem II reaction center protein I, Photosystem II reaction center protein J, Photosystem II reaction center protein K, Photosystem II reaction center protein L, Photosystem II reaction center protein M, Extrinsic protein in photosystem II, Photosystem II reaction center protein T, Extrinsic protein in photosystem II, Cytochrome c-550, Photosystem II reaction center protein Ycf12, Photosystem II reaction center X protein, Photosystem II reaction center protein Z, Extrinsic protein in photosystem II, Photosystem II reaction center protein W, Unknown protein 0, Unknown protein 1, Unknown protein 2, Fucoxanthin chlorophyll a/c-binding protein Lhcf1, FCP1, Fucoxanthin chlorophyll a/c-binding protein monomer 1, Fucoxanthin chlorophyll a/c-binding protein monomer 2, Fucoxanthin chlorophyll a/c-binding protein monomer 3

Experimental Information Details

Resolution: 3.8Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C2
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON III (4k x 4k)