EMDB EMD-9731

Single particle reconstruction
3.45Å resolution

Structure of a substrate engaged SecA-SecY protein translocation machine

Map released:
Overview of EMD-9731
Fitted atomic model: 6itc
To be published

Function and Biology Details

Sample name: SecA-SecY complex
Ligands: MAGNESIUM ION, BERYLLIUM TRIFLUORIDE ION, ADENOSINE-5'-DIPHOSPHATE, (1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE
Proteins: SecA-SecY complex, Protein translocase subunit SecA, Protein translocase subunit SecY, Protein translocase subunit SecE, Nanobody, Translocating peptide, Green fluorescent protein, Nanobody

Experimental Information Details

Resolution: 3.45Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 QUANTUM (4k x 4k)