EMDB EMD-9718

Single particle reconstruction
3.37Å resolution

The crosslinked complex of ISWI-nucleosome in the ADP.BeF-bound state

Map released:
Last modified:
Overview of EMD-9718
Fitted atomic model: 6irm
Related EM entry by publication: EMD-9719, EMD-9720
Primary publication:
Structures of the ISWI-nucleosome complex reveal a conserved mechanism of chromatin remodeling.
Yan L, Wu H, Li X, Gao N, Chen Z
Nat. Struct. Mol. Biol. 26 258-266 (2019)
PMID: 30872815

Function and Biology Details

Sample name: ISWI-nucleosome-crosslinked
Ligands: ADENOSINE-5'-DIPHOSPHATE, BERYLLIUM TRIFLUORIDE ION
Nucleic acid: DNA (167-MER)
Proteins: ISWI-nucleosome-crosslinked, ISWI, nucleosome, DNA, Histone H3, Histone H4, Histone H2A, Histone H2B 1.1, DNA (167-MER), ISWI chromatin-remodeling complex ATPase ISW1

Experimental Information Details

Resolution: 3.37Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN
Detector: GATAN K2 QUANTUM (4k x 4k)