EMDB EMD-9646

Single particle reconstruction
2.9Å resolution

Cryo-EM structure of a human intron lariat spliceosome prior to Prp43 loaded (ILS1 complex) at 2.9 angstrom resolution

Map released:
Overview of EMD-9646
Source organism: Human [9606]
Fitted atomic model: 6id0
Related EM entry by publication: EMD-9645, EMD-9647
Primary publication:
Structures of the human spliceosomes before and after release of the ligated exon.
Zhang X, Zhan X, Yan C, Zhang W, Liu D, Lei J, Shi Y
Cell Res. - (2019)
PMID: 30728453

Function and Biology Details

Sample name: Human Intron Lariat Spliceosome
Ligands: INOSITOL 1,2,3,4,5,6-HEXAKISPHOSPHATE, GUANOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION, ZINC ION
Nucleic acids: U5snRNA, U6snRNA, pre-mRNA, U2snRNA
Proteins: Human Intron Lariat Spliceosome, Pre-mRNA-processing-splicing factor 8, 116 kDa U5 small nuclear ribonucleoprotein component, U5 small nuclear ribonucleoprotein 40 kDa protein, Crooked neck-like protein 1, Cell division cycle 5-like protein, Pre-mRNA-splicing factor SYF2, Protein BUD31 homolog, Pre-mRNA-splicing factor RBM22, Spliceosome-associated protein CWC15 homolog, SNW domain-containing protein 1, Peptidyl-prolyl cis-trans isomerase-like 1, Pleiotropic regulator 1, Pre-mRNA-processing factor 17, CWF19-like protein 2, Pre-mRNA-splicing factor SYF1, Small nuclear ribonucleoprotein Sm D3, Small nuclear ribonucleoprotein-associated protein, Small nuclear ribonucleoprotein Sm D1, Small nuclear ribonucleoprotein Sm D2, Small nuclear ribonucleoprotein F, Small nuclear ribonucleoprotein E, Small nuclear ribonucleoprotein G, Pre-mRNA-processing factor 19, Pre-mRNA-splicing factor SPF27, U2 small nuclear ribonucleoprotein A', U2 small nuclear ribonucleoprotein B'', RNA helicase aquarius, Peptidyl-prolyl cis-trans isomerase E

Experimental Information Details

Resolution: 2.9Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)