EMDB EMD-9645

Single particle reconstruction
3.0Å resolution

Cryo-EM structure of a human post-catalytic spliceosome (P complex) at 3.0 angstrom

Map released:
Overview of EMD-9645
Source organism: Human [9606]
Fitted atomic model: 6icz
Related EM entry by publication: EMD-9646, EMD-9647
Primary publication:
Structures of the human spliceosomes before and after release of the ligated exon.
Zhang X, Zhan X, Yan C, Zhang W, Liu D, Lei J, Shi Y
Cell Res. - (2019)
PMID: 30728453

Function and Biology Details

Sample name: Human Post_catalytic Spliceosome
Ligands: INOSITOL 1,2,3,4,5,6-HEXAKISPHOSPHATE, GUANOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION, ZINC ION, ADENOSINE-5'-TRIPHOSPHATE
Nucleic acids: U5snRNA, U6snRNA, pre-mRNA, U2snRNA
Proteins: Human Post_catalytic Spliceosome, Protein mago nashi homolog 2, RNA-binding protein 8A, Eukaryotic initiation factor 4A-III, Protein CASC3, Pre-mRNA-processing-splicing factor 8, 116 kDa U5 small nuclear ribonucleoprotein component, U5 small nuclear ribonucleoprotein 40 kDa protein, Crooked neck-like protein 1, Cell division cycle 5-like protein, Pre-mRNA-splicing factor SYF2, Protein BUD31 homolog, Pre-mRNA-splicing factor RBM22, Spliceosome-associated protein CWC15 homolog, SNW domain-containing protein 1, Peptidyl-prolyl cis-trans isomerase-like 1, Pleiotropic regulator 1, Serine/arginine repetitive matrix protein 2, Pre-mRNA-splicing factor CWC22 homolog, Pre-mRNA-processing factor 17, PRKR-interacting protein 1, Pre-mRNA-splicing factor SLU7, Pre-mRNA-splicing factor SYF1, Peptidyl-prolyl cis-trans isomerase E, RNA helicase aquarius, Small nuclear ribonucleoprotein Sm D3, Small nuclear ribonucleoprotein-associated protein, Small nuclear ribonucleoprotein Sm D1, Small nuclear ribonucleoprotein Sm D2, Small nuclear ribonucleoprotein F, Small nuclear ribonucleoprotein E, Small nuclear ribonucleoprotein G, U2 small nuclear ribonucleoprotein A', U2 small nuclear ribonucleoprotein B'', ATP-dependent RNA helicase DHX8, Pre-mRNA-splicing factor SPF27, Pre-mRNA-processing factor 19, U5 small nuclear ribonucleoprotein 200 kDa helicase

Experimental Information Details

Resolution: 3.0Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)