EMDB EMD-9400

Single particle reconstruction
3.9Å resolution

Cryo-EM structure of Mcm2-7 double hexamer on dsDNA

Map released:
Last modified:
Overview of EMD-9400
Source organism: Saccharomyces cerevisiae [4932]
Fitted atomic model: 5bk4
Primary publication:
Cryo-EM structure of Mcm2-7 double hexamer on DNA suggests a lagging-strand DNA extrusion model.
Noguchi Y, Yuan Z, Bai L, Schneider S, Zhao G, Stillman B, Speck C, Li H
Proc. Natl. Acad. Sci. U.S.A. 114 E9529-E9538 (2017)
PMID: 29078375

Function and Biology Details

Sample name: Mcm double hexamer bound to double-stranded DNA
Ligand: ADENOSINE-5'-DIPHOSPHATE
Nucleic acids: DNA (60-mer), strand 1, DNA (60-mer), strand 2
Proteins: Mcm double hexamer bound to double-stranded DNA, Mcm double hexamer, synthetic double-stranded DNA, DNA replication licensing factor MCM2, DNA replication licensing factor MCM3, DNA replication licensing factor MCM4, DNA replication licensing factor MCM5, DNA replication licensing factor MCM6, DNA replication licensing factor MCM7

Experimental Information Details

Resolution: 3.9Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)