EMDB EMD-9221

Single particle reconstruction
3.3Å resolution

Cryo-EM structures and dynamics of substrate-engaged human 26S proteasome

Map released:
Overview of EMD-9221
Source organisms:
Fitted atomic model: 6msj
Primary publication:
Cryo-EM structures and dynamics of substrate-engaged human 26S proteasome
Dong YC, Zhang SW, Wu ZL, Li XM, Wang WL, Zhu YN, Stoilova-McPhie S, Lu Y, Finley D, Mao YD
Nature - (2018)

Function and Biology Details

Sample name: Proteasome
Ligands: ZINC ION, ADENOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION, ADENOSINE-5'-DIPHOSPHATE
Proteins: Proteasome, 26S proteasome non-ATPase regulatory subunit 1, 26S proteasome non-ATPase regulatory subunit 3, 26S proteasome non-ATPase regulatory subunit 12, 26S proteasome non-ATPase regulatory subunit 11, 26S proteasome non-ATPase regulatory subunit 6, 26S proteasome non-ATPase regulatory subunit 7, 26S proteasome non-ATPase regulatory subunit 13, 26S proteasome non-ATPase regulatory subunit 4, 26S proteasome non-ATPase regulatory subunit 14, 26S proteasome non-ATPase regulatory subunit 8, 26S proteasome complex subunit SEM1, 26S proteasome non-ATPase regulatory subunit 2, 26S proteasome regulatory subunit 7, Rpt2, NP_002793.2 (26SHA chain B), 26S proteasome regulatory subunit 8, 26S proteasome regulatory subunit 6B, 26S proteasome regulatory subunit 10B, 26S proteasome regulatory subunit 6A, substrate, Proteasome subunit alpha type-6, Proteasome subunit alpha type-2, Proteasome subunit alpha type-4, Proteasome subunit alpha type-7, Proteasome subunit alpha type-5, Proteasome subunit alpha type-1, Proteasome subunit alpha type-3, Proteasome subunit beta type-6, Proteasome subunit beta type-7, Proteasome subunit beta type-3, Proteasome subunit beta type-2, Proteasome subunit beta type-5, Proteasome subunit beta type-1, Proteasome subunit beta type-4, Proteasome subunit alpha type-6

Experimental Information Details

Resolution: 3.3Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)