EMDB EMD-9219

Single particle reconstruction
3.5Å resolution

Cryo-EM structures and dynamics of substrate-engaged human 26S proteasome

Map released:
Overview of EMD-9219
Source organisms:
Fitted atomic model: 6msg
Primary publication:
Cryo-EM structures and dynamics of substrate-engaged human 26S proteasome
Dong YC, Zhang SW, Wu ZL, Li XM, Wang WL, Zhu YN, Stoilova-McPhie S, Lu Y, Finley D, Mao YD
Nature - (2018)

Function and Biology Details

Sample name: Proteasome
Ligands: ZINC ION, ADENOSINE-5'-DIPHOSPHATE, ADENOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION
Proteins: Proteasome, 26S proteasome non-ATPase regulatory subunit 1, 26S proteasome non-ATPase regulatory subunit 3, 26S proteasome non-ATPase regulatory subunit 12, 26S proteasome non-ATPase regulatory subunit 11, 26S proteasome non-ATPase regulatory subunit 6, 26S proteasome non-ATPase regulatory subunit 7, 26S proteasome non-ATPase regulatory subunit 13, 26S proteasome non-ATPase regulatory subunit 4, 26S proteasome non-ATPase regulatory subunit 14, 26S proteasome non-ATPase regulatory subunit 8, 26S proteasome complex subunit SEM1, 26S proteasome non-ATPase regulatory subunit 2, 26S proteasome regulatory subunit 7, 26S proteasome regulatory subunit 4, 26S proteasome regulatory subunit 8, 26S proteasome regulatory subunit 6B, 26S proteasome regulatory subunit 10B, 26S proteasome regulatory subunit 6A, Ubiquitin, substrate, Proteasome subunit alpha type-6, Proteasome subunit alpha type-2, Proteasome subunit alpha type-4, Proteasome subunit alpha type-7, Proteasome subunit alpha type-5, Proteasome subunit alpha type-1, Proteasome subunit alpha type-3, Proteasome subunit beta type-6, Proteasome subunit beta type-7, Proteasome subunit beta type-3, Proteasome subunit beta type-2, Proteasome subunit beta type-5, Proteasome subunit beta type-1, Proteasome subunit beta type-4

Experimental Information Details

Resolution: 3.5Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)