EMDB EMD-9218

Single particle reconstruction
3.3Å resolution

Cryo-EM structures and dynamics of substrate-engaged human 26S proteasome

Map released:
Last modified:
Overview of EMD-9218
Source organisms:
Fitted atomic model: 6mse
Primary publication:
Cryo-EM structures and dynamics of substrate-engaged human 26S proteasome.
Dong Y, Zhang S, Wu Z, Li X, Wang WL, Zhu Y, Stoilova-McPhie S, Lu Y, Finley D, Mao Y
Nature 565 49-55 (2019)
PMID: 30479383

Function and Biology Details

Sample name: Proteasome
Ligands: ZINC ION, ADENOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION, ADENOSINE-5'-DIPHOSPHATE
Proteins: Proteasome, 26S proteasome non-ATPase regulatory subunit 1, 26S proteasome non-ATPase regulatory subunit 3, 26S proteasome non-ATPase regulatory subunit 12, 26S proteasome non-ATPase regulatory subunit 11, 26S proteasome non-ATPase regulatory subunit 6, 26S proteasome non-ATPase regulatory subunit 7, 26S proteasome non-ATPase regulatory subunit 13, 26S proteasome non-ATPase regulatory subunit 4, 26S proteasome non-ATPase regulatory subunit 14, 26S proteasome non-ATPase regulatory subunit 8, 26S proteasome complex subunit SEM1, 26S proteasome non-ATPase regulatory subunit 2, 26S proteasome regulatory subunit 7, 26S proteasome regulatory subunit 4, 26S proteasome regulatory subunit 8, 26S proteasome regulatory subunit 6B, 26S proteasome regulatory subunit 10B, 26S proteasome regulatory subunit 6A, Ubiquitin, substrate, Proteasome subunit alpha type-6, Proteasome subunit alpha type-2, Proteasome subunit alpha type-4, Proteasome subunit alpha type-7, Proteasome subunit alpha type-5, Proteasome subunit alpha type-1, Proteasome subunit alpha type-3, Proteasome subunit beta type-6, Proteasome subunit beta type-7, Proteasome subunit beta type-3, Proteasome subunit beta type-2, Proteasome subunit beta type-5, Proteasome subunit beta type-1, Proteasome subunit beta type-4

Experimental Information Details

Resolution: 3.3Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)