EMDB EMD-9191

Single particle reconstruction
3.4Å resolution

Structure of double-stranded target DNA engaged Csy complex from Pseudomonas aeruginosa (PA-14)

Map released:
Last modified:
Overview of EMD-9191
Source organism: Pseudomonas aeruginosa UCBPP-PA14 [208963]
Fitted atomic model: 6mpu
Primary publication:
Structure Reveals a Mechanism of CRISPR-RNA-Guided Nuclease Recruitment and Anti-CRISPR Viral Mimicry
Rollins MF, Chowdhury S, Carter J, Golden SM, Miettinen HM, Santiago-Frangos A, Faith D, Lawrence MC, Wiedenheft B, Lander GC
Mol.Cell - (2019)

Function and Biology Details

Sample name: Double-stranded target DNA engaged Csy Complex from Pseudomonas aeruginosa (PA-14)
Nucleic acids: CRISPR RNA (60-MER), CRISPR target DNA (44-MER), Non-complementary R-loop DNA strand
Proteins: Double-stranded target DNA engaged Csy Complex from Pseudomonas aeruginosa (PA-14), CRISPR-associated protein Csy2, CRISPR-associated protein Csy3, CRISPR-associated endonuclease Cas6/Csy4, CRISPR-associated protein Csy1

Experimental Information Details

Resolution: 3.4Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Microscope: FEI TALOS ARCTICA
Detector: GATAN K2 SUMMIT (4k x 4k)