EMDB EMD-8996

Single particle reconstruction
3.2Å resolution

CasX-gRNA-DNA(45bp) state I

Map released:
Overview of EMD-8996
Fitted atomic model: 6e79
Related EM entry by publication: EMD-8980
Primary publication:
CasX enzymes comprise a distinct family of RNA-guided genome editors
Liu JJ, Orlova N, Oakes BL, Ma E, Spinner HB, Baney KLM, Jonathan C, Tan D, Knott G, Wanger A, Harrington L, Nogales E, Doudna JA
Nature - (2019)

Function and Biology Details

Sample name: CasX-gRNA-DNA(45bp) state I
Nucleic acids: DNA (35-MER), DNA (35-MER), RNA (112-MER)
Proteins: CasX-gRNA-DNA(45bp) state I, CasX

Experimental Information Details

Resolution: 3.2Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)