EMDB EMD-8980

Single particle reconstruction
3.7Å resolution

CasX ternary complex with 30bp target DNA

Map released:
Overview of EMD-8980
Source organisms:
Fitted atomic model: 6e5o
Related EM entry by publication: EMD-8996
Primary publication:
CasX enzymes comprise a distinct family of RNA-guided genome editors
Liu JJ, Orlova N, Oakes BL, Ma E, Spinner HB, Baney KLM, Jonathan C, Tan D, Knott G, Wanger A, Harrington L, Nogales E, Doudna JA
Nature - (2019)

Function and Biology Details

Sample name: CasX ternary complex with 30bp target DNA
Nucleic acids: DNA (30-MER), DNA (24-mer), RNA (122-MER)
Proteins: CasX ternary complex with 30bp target DNA, CasX

Experimental Information Details

Resolution: 3.7Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)