EMDB EMD-8977

Single particle reconstruction
3.18Å resolution

Cryo-EM structure at 3.2 A resolution of HIV-1 fusion peptide-directed antibody, A12V163-b.01, elicited by vaccination of Rhesus macaques, in complex with stabilized HIV-1 Env BG505 DS-SOSIP, which was also bound to antibodies VRC03 and PGT122

Map released:
Overview of EMD-8977
Source organisms:
Related EM entries by publication: EMD-20189, EMD-20191, EMD-9359
Primary publication:
Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization.
Kong R, Duan H, Sheng Z, Xu K, Acharya P, Chen X, Cheng C, Dingens AS, Gorman J, Sastry M, Shen CH, Zhang B, Zhou T, Chuang GY, Chao CW, Gu Y, Jafari AJ, Louder MK, O'Dell S, Rowshan AP, Viox EG, Wang Y, Choi CW, Corcoran MM, Corrigan AR, Dandey VP, Eng ET, Geng H, Foulds KE, Guo Y, Kwon YD, Lin B, Liu K, Mason RD, Nason MC, Ohr TY, Ou L, Rawi R, Sarfo EK, Schon A, Todd JP, Wang S, Wei H, Wu W, Mullikin JC, Bailer RT, Doria-Rose NA, Karlsson Hedestam GB, Scorpio DG, Overbaugh J, Bloom JD, Carragher B, Potter CS, Shapiro L, Kwong PD, Mascola JR
Cell 178 567-584.e19 (2019)
PMID: 31348886

Function and Biology Details

Sample name: Quaternary complex of HIV-1 Env BG505 DS-SOSIP with antibodies A12V163-b.01, VRC03 and PGT122.
Proteins: Quaternary complex of HIV-1 Env BG505 DS-SOSIP with antibodies A12V163-b.01, VRC03 and PGT122., HIV-1 Env BG505 DS-SOSIP gp120, HIV-1 Env BG505 DS-SOSIP gp41, A12V163-b.01, VRC03, PGT122, HIV-1 Env BG505 DS-SOSIP gp120, HIV-1 Env BG505 DS-SOSIP gp41, A12V163-b.01 Heavy chain, A12V163-b.01 Light chain, VRC03 heavy chain, VRC03 light chain, PGT122 heavy chain, PGT122 light chain

Experimental Information Details

Resolution: 3.18Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C3
Reconstruction software: cryoSPARC
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)