EMDB EMD-8959

Single particle reconstruction
3.59Å resolution

afTMEM16 reconstituted in nanodiscs in the presence of Ca2+ and ceramide 24:0

Map released:
Overview of EMD-8959
Fitted atomic model: 6e1o
Related EM entry by publication: EMD-8931, EMD-8948
Primary publication:
Structural basis of Ca2+-dependent activation and lipid transport by a TMEM16 scramblase.
Falzone ME, Rheinberger J, Lee BC, Peyear T, Sasset L, Raczkowski AM, Eng ET, Di Lorenzo A, Andersen OS, Nimigean CM, Accardi A
Elife 8 - (2019)
PMID: 30648972

Function and Biology Details

Sample name: afTMEM16 reconstituted in nanodiscs in the presence of Ca2+ and ceramide 24:0
Ligands: CALCIUM ION, DODECANE, DECANE, Octadecane, HEXANE
Proteins: afTMEM16 reconstituted in nanodiscs in the presence of Ca2+ and ceramide 24:0, Plasma membrane channel protein (Aqy1), putative

Experimental Information Details

Resolution: 3.59Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)