EMDB EMD-8887

Single particle reconstruction
3.2Å resolution

Vps4p-Vta1p complex with peptide binding to the central pore of Vps4p

Map released:
Overview of EMD-8887
Source organism: Saccharomyces cerevisiae [4932]
Fitted atomic model: 6ap1
Primary publication:
The AAA ATPase Vps4 binds ESCRT-III substrates through a repeating array of dipeptide-binding pockets.
Han H, Monroe N, Sundquist WI, Shen PS, Hill CP
Elife 6 - (2017)
PMID: 29165244

Function and Biology Details

Sample name: Vps4-Vta1 complex
Ligands: ADENOSINE-5'-DIPHOSPHATE, BERYLLIUM TRIFLUORIDE ION, MAGNESIUM ION
Proteins: Vps4-Vta1 complex, Vacuolar protein sorting-associated protein 4,Protein hcp1, ACE-ASP-GLU-ILE-VAL-ASN-LYS-VAL-LEU-NH2, Vacuolar protein sorting-associated protein VTA1

Experimental Information Details

Resolution: 3.2Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 (4k x 4k)