EMDB EMD-8777

Single particle reconstruction
6.9Å resolution

Pol I Initial Transcribing Complex with a truncated scaffold at 6.9 angstrom

Map released:
Last modified:
Overview of EMD-8777
Source organism: Saccharomyces cerevisiae S288c [559292]
Related EM entries by publication: EMD-8771, EMD-8772, EMD-8773, EMD-8774, EMD-8775, EMD-8776
Primary publication:
Structural mechanism of ATP-independent transcription initiation by RNA polymerase I.
Han Y, Yan C, Nguyen THD, Jackobel AJ, Ivanov I, Knutson BA, He Y
Elife 6 - (2017)
PMID: 28623663

Function and Biology Details

Sample name: RNA Polymerase I Initial Transcribing Complex with a truncated scaffold
Nucleic acids: RNA, template strand DNA, upstream non-template strand DNA, downstream non-template strand DNA
Proteins: RNA Polymerase I Initial Transcribing Complex with a truncated scaffold, DNA-directed RNA polymerase I subunit RPA190, DNA-directed RNA polymerase I subunit RPA135, DNA-directed RNA polymerases I and III subunit RPAC1, DNA-directed RNA polymerase I subunit RPA14, DNA-directed RNA polymerases I, II, and III subunit RPABC1, DNA-directed RNA polymerases I, II, and III subunit RPABC2, DNA-directed RNA polymerase I subunit RPA43, DNA-directed RNA polymerases I, II, and III subunit RPABC3, DNA-directed RNA polymerase I subunit RPA12, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerases I and III subunit RPAC2, DNA-directed RNA polymerases I, II, and III subunit RPABC4, DNA-directed RNA polymerase I subunit RPA49, DNA-directed RNA polymerase I subunit RPA34, RNA polymerase I-specific transcription initiation factor RRN6, RNA polymerase I-specific transcription initiation factor RRN7, RNA polymerase I-specific transcription initiation factor RRN11

Experimental Information Details

Resolution: 6.9Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Microscope: JEOL 3200FS
Detector: GATAN K2 SUMMIT (4k x 4k)