EMDB EMD-8774

Single particle reconstruction
4.2Å resolution

RNA Polymerase I Initial Transcribing Complex State 1

Map released:
Last modified:
Overview of EMD-8774
Fitted atomic model: 5w64
Related EM entries by publication: EMD-8771, EMD-8772, EMD-8773, EMD-8775, EMD-8776, EMD-8777
Primary publication:
Structural mechanism of ATP-independent transcription initiation by RNA polymerase I.
Han Y, Yan C, Nguyen THD, Jackobel AJ, Ivanov I, Knutson BA, He Y
Elife 6 - (2017)
PMID: 28623663

Function and Biology Details

Sample name: RNA Polymerase I Initial Transcribing Complex State 1
Ligand: ZINC ION
Nucleic acids: RNA, non-template strand DNA, template strand DNA
Proteins: RNA Polymerase I Initial Transcribing Complex State 1, DNA-directed RNA polymerase I subunit RPA190, DNA-directed RNA polymerase I subunit RPA135, DNA-directed RNA polymerases I and III subunit RPAC1, DNA-directed RNA polymerase I subunit RPA14, DNA-directed RNA polymerases I, II, and III subunit RPABC1, DNA-directed RNA polymerases I, II, and III subunit RPABC2, DNA-directed RNA polymerase I subunit RPA43, DNA-directed RNA polymerases I, II, and III subunit RPABC3, DNA-directed RNA polymerase I subunit RPA12, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerases I and III subunit RPAC2, DNA-directed RNA polymerases I, II, and III subunit RPABC4, DNA-directed RNA polymerase I subunit RPA49, DNA-directed RNA polymerase I subunit RPA34, RNA polymerase I-specific transcription initiation factor RRN6, RNA polymerase I-specific transcription initiation factor RRN7, RNA polymerase I-specific transcription initiation factor RRN11

Experimental Information Details

Resolution: 4.2Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)