EMDB EMD-8642

Single particle reconstruction
3.9Å resolution

Cryo-EM structure of ERAD-associated E3 ubiquitin-protein ligase component HRD3

Map released:
Last modified:
Overview of EMD-8642
Fitted atomic model: 5v7v
Related EM entries by publication: EMD-8637, EMD-8638, EMD-8639
Primary publication:
Cryo-EM structure of the protein-conducting ERAD channel Hrd1 in complex with Hrd3.
Schoebel S, Mi W, Stein A, Ovchinnikov S, Pavlovicz R, DiMaio F, Baker D, Chambers MG, Su H, Li D, Rapoport TA, Liao M
Nature 548 352-355 (2017)
PMID: 28682307

Function and Biology Details

Sample name: Hrd1/Hrd3 complex
Ligand: 2-acetamido-2-deoxy-beta-D-glucopyranose
Proteins: Hrd1/Hrd3 complex, ERAD-associated E3 ubiquitin-protein ligase component HRD3

Experimental Information Details

Resolution: 3.9Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)