EMDB EMD-8624

Single particle reconstruction
3.4Å resolution

Cryo EM structure of anti-CRISPRs, AcrF1 and AcrF2, bound to type I-F crRNA-guided CRISPR surveillance complex

Map released:
Last modified:
Overview of EMD-8624
Fitted atomic model: 5uz9
Primary publication:
Structure Reveals Mechanisms of Viral Suppressors that Intercept a CRISPR RNA-Guided Surveillance Complex.
Chowdhury S, Carter J, Rollins MF, Golden SM, Jackson RN, Hoffmann C, Nosaka L, Bondy-Denomy J, Maxwell KL, Davidson AR, Fischer ER, Lander GC, Wiedenheft B
Cell 169 47-57.e11 (2017)
PMID: 28340349

Function and Biology Details

Sample name: Anti-CRISPRs AcrF1 and AcrF2 bound P. aeruginosa crRNA-guided CRISPR surveillance(Csy)complex.
Nucleic acid: CRISPR RNA (60-MER)
Proteins: Anti-CRISPRs AcrF1 and AcrF2 bound P. aeruginosa crRNA-guided CRISPR surveillance(Csy)complex., CRISPR-associated protein Csy1, CRISPR-associated protein Csy2, CRISPR-associated protein Csy3, Anti-CRISPR protein Acr30-35, Anti-CRISPR protein 30, CRISPR-associated endonuclease Cas6/Csy4

Experimental Information Details

Resolution: 3.4Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)