EMDB EMD-8596

Single particle reconstruction
3.5Å resolution

70S ribosome complex with dnaX mRNA stemloop and E-site tRNA ("in" conformation)

Map released:
Last modified:
Overview of EMD-8596
Fitted atomic model: 5uq7
Related EM entry by publication: EMD-8597
Primary publication:
Alternative Mode of E-Site tRNA Binding in the Presence of a Downstream mRNA Stem Loop at the Entrance Channel.
Zhang Y, Hong S, Ruangprasert A, Skiniotis G, Dunham CM
Structure 26 437-445.e3 (2018)
PMID: 29456023

Function and Biology Details

Sample name: 70S-dnaX complex
Ligands: ZINC ION, IRON/SULFUR CLUSTER
Nucleic acids: 23S rRNA, 5S rRNA, 16S rRNA, P-site tRNA fMet, E-site tRNA Phe, messenger RNA
Proteins: 70S-dnaX complex, 50S ribosomal protein L2, 50S ribosomal protein L3, 50S ribosomal protein L4, 50S ribosomal protein L5, 50S ribosomal protein L6, 50S ribosomal protein L9, 50S ribosomal protein L13, 50S ribosomal protein L14, 50S ribosomal protein L15, 50S ribosomal protein L16, 50S ribosomal protein L17, 50S ribosomal protein L18, 50S ribosomal protein L19, 50S ribosomal protein L20, 50S ribosomal protein L21, 50S ribosomal protein L22, 50S ribosomal protein L23, 50S ribosomal protein L24, 50S ribosomal protein L25, 50S ribosomal protein L27, 50S ribosomal protein L28, 50S ribosomal protein L29, 50S ribosomal protein L30, 50S ribosomal protein L31, 50S ribosomal protein L32, 50S ribosomal protein L33, 50S ribosomal protein L34, 50S ribosomal protein L35, 50S ribosomal protein L36, 30S ribosomal protein S2, 30S ribosomal protein S3, 30S ribosomal protein S4, 30S ribosomal protein S5, 30S ribosomal protein S6, 30S ribosomal protein S7, 30S ribosomal protein S8, 30S ribosomal protein S9, 30S ribosomal protein S10, 30S ribosomal protein S11, 30S ribosomal protein S12, 30S ribosomal protein S13, 30S ribosomal protein S14 type Z, 30S ribosomal protein S15, 30S ribosomal protein S16, 30S ribosomal protein S17, 30S ribosomal protein S18, 30S ribosomal protein S19, 30S ribosomal protein S20, 30S ribosomal protein Thx, 50S ribosomal protein L1

Experimental Information Details

Resolution: 3.5Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 (4k x 4k)