EMDB EMD-8586

Single particle reconstruction
4.38Å resolution

CryoEM structure of E.coli RNA polymerase elongation complex

Map released:
Last modified:
Overview of EMD-8586
Source organisms:
Fitted atomic model: 5upc
Related EM entry by publication: EMD-8584, EMD-8585
Primary publication:
Structural basis of transcription arrest by coliphage HK022 nun in an Escherichia coli RNA polymerase elongation complex.
Kang JY, Olinares PD, Chen J, Campbell EA, Mustaev A, Chait BT, Gottesman ME, Darst SA
Elife 6 - (2017)
PMID: 28318486

Function and Biology Details

Sample name: E.coli RNA polymerase ternary elongation complex
Ligands: MAGNESIUM ION, ZINC ION
Nucleic acids: DNA (29-MER), DNA (29-MER), RNA (5'-R(*GP*CP*A*UP*UP*CP*AP*AP*AP*GP*CP*GP*GP*AP*GP*AP*GP*GP*UP*A)-3')
Proteins: E.coli RNA polymerase ternary elongation complex, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', DNA-directed RNA polymerase subunit omega

Experimental Information Details

Resolution: 4.38Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)