EMDB EMD-8585

Single particle reconstruction
4.05Å resolution

CryoEM structure of crosslinked E.coli RNA polymerase elongation complex

Map released:
Last modified:
Overview of EMD-8585
Fitted atomic model: 5upa
Related EM entry by publication: EMD-8584, EMD-8586
Primary publication:
Structural basis of transcription arrest by coliphage HK022 nun in an Escherichia coli RNA polymerase elongation complex.
Kang JY, Olinares PD, Chen J, Campbell EA, Mustaev A, Chait BT, Gottesman ME, Darst SA
Elife 6 - (2017)
PMID: 28318486

Function and Biology Details

Sample name: Crosslinked E.coli RNA polymerase ternary elongation complex
Ligands: MAGNESIUM ION, ZINC ION
Nucleic acids: DNA (29-MER), DNA (29-MER), RNA (5'-R(*GP*CP*A*UP*UP*CP*AP*AP*AP*GP*CP*GP*GP*AP*GP*AP*GP*GP*UP*A)-3')
Proteins: Crosslinked E.coli RNA polymerase ternary elongation complex, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', DNA-directed RNA polymerase subunit omega

Experimental Information Details

Resolution: 4.05Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)