EMDB EMD-8558

Single particle reconstruction
15.6Å resolution

Negative stain reconstruction of Cas1-Cas2/3 complex of type I-F CRISPR system from Pseudomonas aeruginosa (PA14)

Map released:
Last modified:
Overview of EMD-8558
Source organism: Pseudomonas aeruginosa PA14 [652611]
Primary publication:
Cas1 and the Csy complex are opposing regulators of Cas2/3 nuclease activity.
Rollins MF, Chowdhury S, Carter J, Golden SM, Wilkinson RA, Bondy-Denomy J, Lander GC, Wiedenheft B
Proc. Natl. Acad. Sci. U.S.A. 114 E5113-E5121 (2017)
PMID: 28438998

Function and Biology Details

Sample name: Complex containing dimer of Cas2/3 protein and tetramer of Cas1 protein.
Protein: Complex containing dimer of Cas2/3 protein and tetramer of Cas1 protein.

Experimental Information Details

Resolution: 15.6Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Microscope: FEI TECNAI SPIRIT
Detector: TVIPS TEMCAM-F416 (4k x 4k)