EMDB EMD-8540

Single particle reconstruction
3.9Å resolution

Structural basis of MCM2-7 replicative helicase loading by ORC-Cdc6 and Cdt1

Map released:
Last modified:
Overview of EMD-8540
Fitted atomic model: 5udb
Primary publication:
Structural basis of Mcm2-7 replicative helicase loading by ORC-Cdc6 and Cdt1.
Yuan Z, Riera A, Bai L, Sun J, Nandi S, Spanos C, Chen ZA, Barbon M, Rappsilber J, Stillman B, Speck C, Li H
Nat. Struct. Mol. Biol. 24 316-324 (2017)
PMID: 28191893

Function and Biology Details

Sample name: ORC-Cdc6-Cdt1-MCM2-7
Ligand: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER
Nucleic acids: DNA (39-MER), DNA (39-MER)
Proteins: ORC-Cdc6-Cdt1-MCM2-7, DNA replication licensing factor MCM2, DNA replication licensing factor MCM3, DNA replication licensing factor MCM4, Minichromosome maintenance protein 5, DNA replication licensing factor MCM6, DNA replication licensing factor MCM7, Cell division cycle protein CDT1, Cell division control protein 6, Origin recognition complex subunit 1, Origin recognition complex subunit 2, Origin recognition complex subunit 3, Origin recognition complex subunit 4, Origin recognition complex subunit 5, Origin recognition complex subunit 6

Experimental Information Details

Resolution: 3.9Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)